Staining of sections

Heyn, A. N. J. (1966). The microcrystalline structure of cellulose in cell walls of cotton, ramie, and jute fibers as revealed by negative staining of sections./. Cell. Biol. 29 181-197. 

Substrates are only soluble to a limited extent in aqueous buffers. The use of mixed aqueous/organic buffers is possible. These solvent systems can allow significantly greater amounts of substrates to be incorporated into solution and also allow their use in microplate ELISAs. Partially or totally insoluble products can be used in variants of ELISA, e.g., in the staining of sections in immunohistochemistry in which insoluble products localize the area of antigen or antibody reaction. 

Fig. 7. TEM (positive staining) of sections of collagenous tissue, sheep cruciate ligament, showing interstitial collagen fibrils in (A) longitudinal and (B) cross sectional orientations. Photographs courtesy of Jacinta F. White.

Paine et al. [99] tried different stabilizers [i.e., hydroxy propylcellulose, poly(N-vinylpyrollidone), and poly(acrylic acid)] in the dispersion polymerization of styrene initiated with AIBN in the ethanol medium. The direct observation of the stained thin sections of the particles by transmission electron microscopy showed the existence of stabilizer layer in 10-20 nm thickness on the surface of the polystyrene particles. When the polystyrene latexes were dissolved in dioxane and precipitated with methanol, new latex particles with a similar surface stabilizer morphology were obtained. These results supported the grafting mechanism of stabilization during dispersion polymerization of styrene in polar solvents. 

Neuroanatomists have taken advantage of the phenomenon of fast retrograde transport to locate remote nerve cell bodies in the CNS of an experimental animal that are connected to an identified axonal fiber tract whose origin is uncertain. The tracer material [purified horseradish peroxidase (HRP) enzyme] is injected in the region of the axon terminals, where it is taken up by endocytosis and then is carried by retrograde axonal transport over a period of several hours to days back to the nerve cell body. The animal is sacrificed, and the enzyme tracer is localized by staining thin sections of the brain for peroxidase activity. 

Two distinct patterns of repeats were observed by electron microscopy of sectioned, negatively stained, frozen-hyd rated, or freeze-fractured specimens of Ca -ATPase crystals that represent different projections of the same structure... 

Fig. 10.2. Immunogold staining of an ultra-thin section of an immature Acanthocheilonema viteae uterine mf with mAb 24-4. Note that A. viteae chitinase is present in the cuticle (arrowheads), but not on the surface. (Photograph W. Rudin.)...

Shi SR, Cote RJ, Yang C, et al. Development of an optimal protocol for antigen retrieval a test battery approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. J. Pathol. 1996 179 347-352. 

Evers P, Uylings HB. Effects of microwave pretreatment on immunocytochemical staining of vibratome sections and tissue blocks of human cerebral cortex stored in formaldehyde fixative for long periods. /. Neurosci. Methods 1994 55 163-172. 

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