Chemosensitivity testing

Carmichael J, DeGraff WG, Gazdar AF et al. (1987) Evaluation of a tetrazolium-based semiauto-mated colorimetric assay assessment of chemosensitivity testing. Cancer Res. 47 936-942. 

The MTT assay was initially developed as a quantitative assay for cell survival and proliferation, not as an in vitro assay for chemosensitivity testing. Further study was required to ascertain if the method accurately predicted the in vivo antitumor activities of anticancer agents. Shimoyama et al. [189] studied the predictability of the MTT assay with respect to a clonogenic assay (Sect. 4.1.1.3.) and showed excellent reproducibility and a close correlation to the in vivo predictability rate of the clonogenic assay. Another study [190] also showed that the MTT assay closely approximated (90%) the clinical activity of anticancer agents. Many authors have since utilized the MTT assay to determine the efficacy of polymeric anticancer drug conjugates. 

Kobayashi H, Higashiyama M, Minamigawa K, et al. Examination of in vitro chemosensitivity test using collagen gel droplet culture method with colorimetric endpoint quantification. Jpn. J. Cancer Res. 2001 92 203-210. 

There is a need for a rapid chemosensitive test to select the most appropriate drug and optimal dose to cause cytotoxic activity for screening of potential anticancer agents in patients. In this context a number of tests are currently available including the measurement of DNA synthesis by [ HJthymidine incorporation, the measurement of cellular permeability, measurement of cellular metabolism using Alamar Blue, MTT (3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) and the measurement of total cytosolic... 

Link KH, Aigner KR, Kuehn W, et al (1986) Prospective correlative chemosensitivity testing in high-dose intraarterial chemotherapy for liver metastases. Cancer Res 46 4837-4848... 

Link KH, Kornmann M, Safi F, et al (1994) Response prediction in hepatic artery infusion with Fluoropyrimidines using cell culture and polymerase chain reaction techniques. Eur J Surg Oncol 20 317-318 Link KH, Kornmann M, Leder GH, et al (1996) Regional chemotherapy directed by individual chemosensitivity testing in vitro a prospective decision aiding trial. Clin Cancer Res 2 1469-1474... 

Functional high-throughput multifunctional screening of chemosensitive properties of conductive polymers was described recently.44 The authors developed a minimal test procedure consisting of two concentration pulses of an analyte at the same concentration and a sequence of concentration pulses at increased concentrations. Automated analysis of the materials responses has given the following information ... 

The compounds were first tested against two panels of human tumour cell lines. The primary cell panel consisted of cells from tumours representative of different tissue types and with different chemosensitivities to cisplatin . Differential cytotoxicity, as opposed to non-selective toxicity, was used as an indicator of potential antitumour activity of test compounds . The second panel was disease-oriented and consisted of cell lines established from human ovarian tumours. This panel included two pairs of cisplatin-sensitive and cisplatin-resistant hnes, with defined mechanisms of resistance (CHl/CHl-R, A2780/A2780-R), and also two inherently resistant cell hnes (HX62 and SK-OV-3). This panel has been used to identify agents with the abihty to circumvent cisplatin resistance . ... 

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