Plating medium

The filter floor and cloth, woven mesh screen or sintered metal plate medium... 

Data on the toxicity of TCPM and TCPMe are very scarce. Michaels and Lewis [2] have reported a toxicity study on five azo and triphenylmethane dyes. In that study Basic Violet 3, with a structure in which the basic structure of TCPM can be recognized (Fig. 2), shows the highest toxicity a survival rate of 20.7 6.6% at a dye concentration of 5.0 mg/1 of microbiota in a plating medium incorporated with dye. 

Different dilutions of the chemoattractant in plating medium are placed into the lower compartment, and the chamber is incubated at 37° C for a period of time that usually ranges between 4 and 24 h. The assay is also suitable for the evaluation of haptotactic stimulation. In this case the attractant (usually extracellular membrane molecules) is insolubilized on the bottom surface of the filter by letting the filter float on the surface of a solution (either PBS or carbonate buffer, 50-100 mM, pH 9.6) containing the desired molecule at concentrations ranging between 10 and 120 jU-g/ml for 2-18 h at... 

Figure 1. Time course of medium glucose and lactate concentrations ofLLC-PKI ceiis under conventionai static conditions (A) and with continuous medium renewai (B). (A) LLC-PKi cells were grown to confluence and on 24 well plates, medium was removed from wells every two hours. Two medium replenishment cycles were conducted [r], (B) LLC-PK1 cells were cultured to confluence on micropourus filters and transferred to the EpiFlow perfusion device. Medium perfusion was 2 mi/h. Sampies in the outflowing medium werecoiiectedatreguiarintervais. Giucose and lactate were measured using colorimetric assays. Results represent the mean SEM from 3 experiments. For more information see Felder et. al. 2002 [72].

Pulsed sieve plate Medium Large" Medium Good Excellent [G2]... 

During incubation, prepare P-BSK solid-plating medium and pour the 15-mL bottom layer. 

For each dilution, add 100 pL of cells to 15 mL of the P-BSK-plating medium and pour onto the previously prepared bottom layers. Allow to solidily at room... 

Chem. Descrip. Propylene carbonate CAS m32-7 EINECS/ELINCS 20S 572-1 Uses Solvent, reactant, plastidzer for fibers, textiles, dyeing, plastics and resins, gas treating, aromatic hydrocarbon extraction, metal extraction, surf, coatings (paints, varnishes, adhesives, plastics, epoxies, mastics), foundry sand binder, lubricants, electrolytes, cosmetics (polar additive for montmorillonite or bentonite clay gellants) fire extinguishing compds. antifoam for antifreeze hydraulic brake fluids as plating medium... 

Spectrum Analysis. No. 3 Spectrum Analysis. No. Projector Slide Plate. Contrast Projector Slide Plate, Medium 1 High Speed Infrared Film Metallographic M Plate 33 Posili /e Plate IRIX Panchromatic. Type B... 

Neuronal plating medium OptiMEM-1 reduced-serum medium (GIBCO, 31985), 20 mM glucose. 

Wash the specimens three times with 5 ml of neuronal plating medium. 

Triturate the hippocampi completely in 10 ml of neuronal plating medium by pipetting up and down approximately 30 times with a 10-ml disposable pipette. To remove chunks of tissues, pass the triturated solution through a 100-//m filter (Cell Strainer, 352360, BD Biosciences, Bedford, MA, USA) and determine the cell density with a hemocytomer. 

Plate the cells onto a laminin- and poly-D-lysine-coated culture dish at a density of 2 x 10 cells in 2 ml of OptiMEM I/glucose solution. Allow the cells to adhere to the dish for 1-2 h in CO2 incubator (37°, 5% CO2). After 1-2 h, exchange the neuronal plating medium with 3 ml of the neuronal maintenance medium and incubate the plates in CO2 incubator. In case the culture is maintained for more than one week, half of the medium is exchanged with the fresh culture medium every five days after plating. 

Add 36% w/w hydrochloric acid dropwise to stock solution C until the solution is clear (approximately 0.5 ml. is required). From these stock solutions, which can be stored in a refrigerator, the agar plate medium is prepared in the following manner. Mbc in the order given ... 

Preparations such as lozenges containing sugars as diluents yield erroneous avssays by the standard method. To overcome this, add either an equivalent amount of glucose to the standard penicillin solution or 0 5 per cent of glucose to the assay plate medium. 

In 1948, Fries and Kihlman in Sweden reported the production of auxotrophic mutations in the ascomycete Ophiostoma multiannulatum by prolonged immersion of the conidia in solutions of caffeine (0.2%) or theophylline (0.6%). Either treatment yielded about 1% auxotrophs at survival levels of about 0.2% control mutation frequencies were very low. Qualitatively, the results were especially interesting because they showed a difference between the mutational spectra induced by these two purines on the one hand and X-irradiation on the other. This was most striking for inositol requirers, which formed less than 5% of radiation-induced auxotrophs but more than 40% of purine-induced ones. This is one of the rare cases of mutagen specificity for a whole locus or biochemical pathway. Like similar cases that were subsequently reported from the same laboratory, it may rest on selective effects of the plating medium, amplified by the previous treatment of the plated conidia. 

The problem of mutagen specificity as far as plating-medium effects are concerned has to be borne in mind for a proper evaluation of the data obtained. Actually, it is the complications introduced by these phenomena which make a simple cookbook type of approach to routine screening for mutagenicity impossible. 

Plating medium MEM (Sigma Chemicals) supplemented with horse serum (10 % v/v, Invitrogen ), L-glutamine (2 mM), and sodium pyruvate (1 mM) (Invitrogen ). 

The preparation of primary cultures of hippocampal neurons is briefly described here. Further details can be found in [ 17], Animals are euthanized using CO2 for 10 min (reeNote 2). Tissue is then trypsinized (0.25 % v/v trypsin in lx HBSS +10 mM HEPES) and mechanically dissociated in lx HBSS containing 10 mM HEPES. Neurons are plated at a density of 2.3x10 cells/cm onto glass coverslips precoated with 80 pg/mLpoly-o, L-ornithine in plating medium. After attachment for 2-3 h, cells are incubated in maintenance medium for up to 3 weeks at 37 °C in a 5 % CO2 humidified incubator. Each week, one-fifili of the culture medium volume is renewed with fresh medium. 

This pattern of plate/medium-frame-medium/plate/medium-frame would be repeated along the filter until sufficient filtration area had been built into the systan, and the filter would then be closed by bringing up the queen (movable end) plate, carrying a sheet of medium, to be clamped against the last frame of the series. This whole array would then be pressed hard up against the fixed king plate at the other end of the filter (also carrying a sheet of filter medium), to create the whole filler press, formally termed a plate and frame (filter) press. 

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