Glass coverslip

Both dynein and MAP2 interact with microtubules at the same binding sites, namely, the C termini of a- and p-tubulin. Also, MAP2 inhibits the microtubule-activated ATPase of dynein and prevents microtubule gliding on dynein-coated glass coverslips. Thus, MAP2 and other fibrous MAPs may be regulators of microtubule-based motility in vivo (Paschal et al., 1989). 

Figure 13, A schematic diagram of the motility assay. Myosin molecules (HMM or S-1 are also used) stick to glass coverslips coated with nitrocellulose. Actin, in solution, is then added to the glass coverslip and it binds to the myosin molecules. When ATP is added, actin can move over the surface, propelled by the myosin molecules.

Grasp one end of a dust-free 22 mm X 40 mm microscope glass coverslip with forceps. Lower one end near the cDNA probe until it touches the surface outside the printed area and slowly lower the opposite end of the coverslip onto the slide. The solution will spread across the entire print area beneath the coverslip. Use a yellow tip to carefully adjust the position of the coverslip over the printed area. Large air bubbles can be moved away from the hybridization area by a gentle tapping on the coverslip with a yellow tip. Small air bubbles will be released during hybridization. 

Figure 22. Human embryonic kidney cells (A), rat vascular smooth muscle cells (B, C) and human osteoblast-like MG 63 cells (D) in cultures on micropattemed surfaces. A, B PTFE irradiated with UV light produced by a Xe2 -excimer lamp for 30 min in an ammonia atmosphere through a mask with holes 100 pm in diameter and center-to-center distance 300 pm C PE irradiated with Ar ions (energy 150 keV, ion dose lO ions/cm ) through a mask with holes 100 pm in diameter and center-to-center distance 200 pm fullerenes Qo deposited through a mask with rectangular holes with an average size of 128 3 pm per 98 8 pm on glass coverslips. Day 7 after seeding. A native cells in an inverted phase-contrast microscope B, C cells stained with hematoxylin and eosin, Olympus microscope IX 50 D cells stained with fluorescence-based LIVE/DEAD viability/cytotoxicity kit (Invitrogen), Olympus microscope IX 50. Bars 300 pm (A), 200 pm (B, D), Imm (C) [10,11].

Figure 29. Fiuman osteoblast-like MG 63 cells in cultures on material surfaces modified with carbon nanoparticles. A fullerene Cgo layers deposited on carbon fibre-reinforced carbon composites (CFRC), B fullerene C o layers deposited on microscopic glass coverslips, C terpolymer of polytetrafluoroethylene, polyvinyldifluoride and polypropylene, mixed with 4% of single-wall carbon nanohorns, D the same terpolymer with high crystalline electric arc multi-wall nanotubes, E diamond layer with hierarchically organized micro- and nanostmcture deposited on a Si substrate, F nanocrystalline diamond layer on a Si substrate. Standard control cell culture substrates were represented by a PS culture dish (G) and microscopic glass coverslip (FI). Immunofluorescence staining on day 2 (A) or 3 (B-Fl) after seeding, Olympus epifluorescence microscope IX 50, digital camera DP 70, obj. 20x, bar 100 pm (A, C, D, G,H)or 200 pm (B, E, F) [16].

Quickly overlay the slide with a number-one glass coverslip, and seal the edge with rubber cement. 

Add 30 0 pL of probe containing hybridization solution to each tissue section treated with the in situ RT-PCR reaction protocol. Cover with siliconized glass coverslips, seal with rubber cement. 

The sealed glass coverslip prevents evaporation of reaction mixture during PCR cycling. For sealing ... 

Figure 3.3. TMAFM images of nanovolcanoes formed at the surface of an EDT-TTF-(CONHMe)2 microcrystal after eruption of the CH3CN solvent to the atmosphere. The microcrystal was formed after the deposition of a drop of a saturated solution of EDT-TTF-(CONHMe)2 in CH3CN on a glass coverslip and exposed to ambient conditions, (a) 10 pm x 10 pm and (b) 2 pm x 2 pm. The illumination is set in such a way that the feature appears like a volcano seen by a satellite.

To observe the formation of these structures from above, the experiments were performed in a petri dish with a glass coverslip. In this case, a regular mosaic pattern was observed within 10-15 min of the boundary formation (Fig. 14). 

TERS experiments were performed to study the bacterial cell surface. Biju et al. combined silver island films on glass coverslips with an AFM to investigate the effect of electron-acceptor limitation on the outer cell membrane of Shewanella one.ide.nsis [103]. 

The preferred cells are usually primary hepatocytes in cultures derived from adult male rats whose cells are dispersed and allowed to attach themselves to glass coverslips. 

Fig. 3.6. Various types of flow chambers. A and B are designs used in sorting cytometers (in A the analysis point is in air after the stream has left the flow cell in B analysis occurs within an optically clear region of the chamber itself). C and D are two designs for nonsorting cytometers (in C the stream flows upward through an optically clear region of the chamber in D the stream is directed at an angle across a glass coverslip). Adapted from Pinkel and Stovel (1985).

C6 glioma cells were grown in F-10 medium supplemented with 10% horse serum and 2.5% fetal calf serum. Cells were plated on glass coverslips (22 mm2) and used when they reached 50% confluence. (LCM, lipid-coated microbubbles S.D., standard deviation.)... 

Figure 4.3 Schematic drawings illustrate two mated window chamber plates, one containing a planar sensor and its connector (a), while the companion plate has an open ring for the placement of a glass coverslip (b).

Adherent cells have also been patterned using cell-adhesive and non-cell-adhesive microdomains created on a glass coverslip. This coverslip was then bonded with a PDMS channel layer for cell patterning [857]. For instance, endothelia or astrocyte-neuron cocultures were plated only on the cell-adhesive poly-L-lysine microdomains, but not on the non-adhesive agarose (1%) microdomains. Subsequently, calcium wave measurements were made to study calcium signaling of cells in two modes (1) within confluent cell microdomains, and (2) across neighboring, but spatially disconnected, microdomains [857]. 

Consider an initially uniform suspension of cells covering to a depth L the top surface of a plane, horizontal, glass coverslip. The distribution of cells c(x, t) must satisfy the diffusion equation, modified by inclusion of the... 

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