Phenol water-saturated

Add 4 ml acid phenol (water saturated, low pH [e.g., Sigma P4682]). Vortex well. 

Stop reaction by adding 10 ftl phenol (water saturated)... 

Phenol, water-saturated (lower layer used) Ue)... 

Discard the supernatant and resuspend the pellet in 650 1 RNase-free water. Add an equal volume of water-saturated phenol chloroform (5 1), pH 5.2. Vortex vigorously and spin at top speed for 5 min at room temperature. Take 500 pi of the aqueous phase into a new microtube tube. 

El Khadem18 reported that D-glucosone gives a single spot when developed with collidine-water or phenol-water, Rp values of 0.21 and 0.13 respectively, being recorded. It was stated18 that no movement of the osone occurs on development with butanol saturated with water. Homo-... 

Materials Required Thyroid gland dried 1.0 g anhydrous potassium carbonate 17.0 g bromine solution (9.6 ml of Br2 and 30 g of KBr in 100 ml DW) 7.0 ml dilute phosphoric acid (10% w/v) 42.0 ml starch iodide paper phenol solution (saturated solution of phenol in water) 5.0 ml potassium iodide solution (10% w/v in water) 0.01 N sodium thiosulphate solution starch solution. 

The use of distribution coefficients for the QSAR treatment of ionizable compounds has been extended to consideration of ion-pair partitioning into biolipid phases. Two experimental methods for determining ion-pair partition coefficients are described. One is a single-phase titration in water-saturated octanol, in which case (for acids) log Pj = log P + pKa - pKa. The other is a two-phase titration (octanol/water) from which the ratio (P + 1)/(Pj + 1) can be calculated. An example outcome is that the uncoupling activity of phenols can be represented by an equation in log instead of log D and pKa. 

Table I. HaIf-Neutralization pH for Benzoic Acids and Phenols in Water-Saturated Octanol.

Water-saturated phenol/chloroform is prepared in the following way. Phenol is fused by heating the botde in hot water (55-60°C). After addition of an excess amount ofwater, they are mixed well and left to allow separation of water from the mixture at room temperature. After water-phenol separation, the same amount of chloroform as the water-saturated phenol is added. These are then mixed well and left again to allow separation. Use the lower layer for phenol/ chloroform extraction. The upper water can be discarded if the volume is too excessive. 

The lipid material precipitated upon mild acid treatment of the Boivin-extracted lipopolysaccharide is here termed a lipoidal precipitate. The fatty-acid profile (Table IV) of hydrolyzates of this material shows little variation between the seven lmmunotype strains. If the original lipopolysaccharides are first treated by phenol—water extraction, and the resultant materials then subjected to hydrolysis to release the lipid, the composition of the latter is significantly different it corresponds closely to the classic composition expected for lipid A. It is noteworthy that material extracted by the phenol—water (Westphal) method is rich in the Csaturated acid and in the hydroxy fatty acids having ten and twelve carbon atoms, whereas the and C g saturated acids present in the lipoidal precipitate, as prepared by the Boivin procedure, are absent or present at much lower levels in the lipid prepared by the Westphal procedure -9). It... 

Water-saturated phenol—1% ammonia—hydrocyanic acid.87 2. Water-saturated s-collidine,27 3. 1-Butanol-acetic acid—water 4 1 5 v/v.87 4. Ethyl acetate-pyridine-water 2 1 2,28 5. Ethyl acetate—acetic acid—water 3 1 3.6. feri-Pentyl alcohol—1-propanol—water 4 1 1.5.108 b Lactone. Free base. 

The phenol used for RNA extraction should be of the highest purity (double distilled), as oxidation products of phenol can cause degradation of RNA. Before use, the phenol is saturated with DEPC-treated water, and the phases are allowed to separate. The pH of the top aqueous layer is tested with pH paper. If phenol is acidic, it is equilibrated with Tris buffer by mixing it with a volume of 1 M Tris-Cl, pH 7.0. After phase separation, the top buffer layer is removed and the phenol is equilibrated twice with water. Additives such as 8-hydroxyquinoline (0.1%) are used in phenol to inhibit the activity of nucleases. Caution should be exercised in the use of phenol, as it is corrosive and a suspected carcinogen. Use in a chemical hood is recommended. Phenol chloroform extraction should be carried out in con-... 

If we consider two liquids A and B and shake together, then some of A dissolves in B, while some of B dissolves in A. We then have two saturated solutions—one of A in B and the other of B in A. On increasing the temperature, the solubility of A increases in B and also that of B in A, in this particular case. As an example, we take the familiar phenol-water system. 

Add 5 mL of water-saturated phenol. Vortex the mixtures thoroughly, then centrifuge at 3,000xg for 30 min at room temperature. 

To the extension mixture from Step 2 add 25 p phenol (redistilled, water-saturated). 

Method 1 takes advantage of the properties of a low melting temperature agarose (Bethesda Research Laboratories, Rockville, Md., USA). The gel slice is melted at 70°C and an equal volume of 10 mM Tris-HCl, (pH 7.6), 0.1 mM-EDTA added and the solution extracted with the same volume of water-saturated phenol in one or more 1.5 ml Eppendorf centrifuge tubes. After brief centrifugation the aqueous layer is removed and the phenol extraction repeated a further 2-3 times. Finally the aqueous phase is extrac-... 

Other solutions TE (10 mM Tris-HCl, pH 8.0,1 mMEDTA) Phenol/CIAA saturated with TE 2 mM dCTP 7.5 M NH4OAc EtOH 70% EtOH, and sterile water. 

For extracting total DNA from large amounts of plant tissue, use the above procedure, omitting the sucrose gradients (i.e., do Steps 1-6 and 10-15). After the 2-propanol extraction and dialysis for 2 hr or longer, use an equal volume of Tris-buffered phenol [prepared just before use by adding 1/10 volume of unbuffered Tris to 1 volume of water-saturated phenol (IBI, New Haven, CT)] to extract DNA twice, followed by an ether extraction. The DNA is then dialyzed as above. 

The first phenolic derivative of ferrocene to be reported, di(l-hy-droxy-3-methyl-cyclopentadienyl) iron, was prepared by the reaction between 3-methyl-2-cyclopentanone, sodamide, and ferrous chloride in liquid ammonia. This compound is remarkably unstable, being extremely sensitive to air. It is a yellow substance which sublimes at 130-140° at 0.1 mm pressure, and is readily soluble in methylene dichloride but insoluble in carbon tetrachloride and in n-heptane. In water saturated with nitrogen a yellow solution is produced which rapidly bec( nes blue-green on exposure to air. The compound forms a benzoate (mp 121-123.5°) which is stable in air. An analogous derivative of the dicyclo-pentadienyl cobalt (III) cation has also been obtained (6). 

Water saturated with 2,4,6-collidine 2. 1-Butanol-acetic acid. 3. Phenol-acetic acid-water. 4. 1-Butanol-ethanol-water. ... 

In water saturated with phenol, adjusted to pH 5. acid-water (3 1 3). 

Water saturated with 2,4,6-collidine 60), 2. 1-butanol-acetic acid (60) 3. phenol-acetic-water ( 5) 4. 1-butanol-ethanol-water 30). 

MtDNAs are extracted from the mixture by adding an equal vol of water saturated phenol and are precipitated with 2.5 vol of ethanol at -20°C overnight. 

Phenol can also be produced by fusing the sodium salt of benzene sulfonic acid NaOH at 320°C, which leads to the formation of sodium phenate, sodium sulfite, and water. Saturation with CO2 yields phenol from the sodium phenate. 

Fig. 6. Co-chromatography of putative hydroxyproline with authentic hydroxy-proline. The putative hydroxyproline was eluted from a chromatogram similar to that shown in Fig. 5, mixed with authentic [ C]hydroxyproline and chromatographed in a phenol ammonia ethanol solvent (water-saturated phenol, 15 ml 0.88 ammonia, 1 ml and ethanol, 4 ml). Strips sliced from the chromatogram were eluted with 0.01 M HCl and the radioactivity in H and C was measured for each eluate.

DeBolt and Kollman used AMBER 4.0 to calculate relative partition coefficients of benzene and phenol between water and hydrated 1-octanol. The calculated A log P for the mutation of phenol to benzene was -0.76 0.39 (experimental, -0.67). On the basis of structural analyses, the authors postulated that water-saturated l-octanoPs capability to serve as a biophase analog is due to the formation of preferentially polar versus nonpolar regions in the media, crudely mimicking the components and perhaps compartmental boundaries found in the body. 

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