N-butanol-acetic acid-water

For the same purpose, Kelly and Bryske used paper impregnated with 0.1N disodium ethylenediaminetetraacetate (EDTA) and two mobile solvents the organic phase from a mixture of n-butanol, ammonia, water (4 1 5) and the organic phase from a mixture of n-butanol, acetic acid, water (4 1 5). Disodium EDTA (0.1N) works as well as Mcllvaine s buffer when it is used to treat the paper in the method of Walton et al. (49). A circular paper chromatographic method also using paper dampened with Mcllvaine s buffer (pH 4.5) was reported by Urx et al. (50). They used a mixture of chloroform and n-butanol (4 1) as the mobile solvent. 

Whatman No.l Ascending n-Butanol/acetic acid/water(2 1 1)... 

Separates monosaccharides and disaccharides. Especially useful for methyl glycosides and sugar alcohols. Gives good separation with mono-, di-, tri- and oligosaccharides. Used as second solvent after n-butanol/acetic acid/water (SG)... 

Figure 10.17 Two-dimensional high voltage electrophoresis and chromatography of amino acids. Paper high voltage electrophoresis (4000 V) in an acetic acid-formic acid buffer at pH 2.0 in the first dimension followed by descending chromatography in the second dimension in an n-butanol-acetic acid-water solvent (12 3 5). The spots were visualized with a ninhydrin-collidine reagent.

Normal-phase TLC using a silica stationary phase was employed for the optimization of the separation of flavonoid content of Matricariae flos (Chamomilla recutita L. Rauschert). Air-dried and powdered plant material was extracted by refluxing for 10 min with methanol. The suspension was filtered, evaporated and the residue was redissolved in methanol. The mobile phases included in the experiments were 1 = ethyl acetate-methylethylketone-formic acid-water (50 30 10 10, v/v) 2 = ethyl acetate-methanol-water (75 15 10 v/v) 3 = ethyl acetate-formic acid-water (80 10 10, v/v) 4 = ethyl acetate-formic acid-water (100 20 30, v/v) 5 = ethyl acetate-formic acid-acetic acid-water (100 11 11 27, v/v) 6 = n-butanol-acetic acid-water (66 17 17, v/v) 7 = ethyl acetate-methanol-formic acid-water (75 10 5 10, v/v) 8 = ethyl acetate-acetic acid-water (80 10 10, v/v). Development was carried out in the linear ascending mode at... 

The diuretic and cholagog effect of Ononis arvensis motivated the development of a new two-dimensional paper chromatographic and TLC method for the measurement of onion in its roots and aerial parts. Samples were dried, ground and extracted with methanol (70 per cent, w/v) for 2 h. The supernatant was diluted and used for TLC separation on a cellulose stationary phase. The first eluent was 3 per cent formic acid, and the second was n-butanol-acetic acid-water (4 1 5, v/v) for both TLC and paper chromatography. Spots were scraped off, extracted with methanol and the absorption was measued at 260 nm. It was found that the average ononin content in roots and aerial parts was 0.153 0.0278 (per cent) and 0.498 0.045 (per cent), respectively. Because of the simplicity, the method was... 

Adsorption chromatography. The concentrated sample in 30 ml eluent (n-butanol/acetic acid/water = 4 1 1 by vol. (Bu0H/HAc/H20)) was applied to a column of 5 g CFl cellulose preswollen in eluent. The column was first eluted with Bu0H/HAc/H20 and three 100-ml fractions collected, then with water and four additional 50-ml fractions collected. 

TLC. Aliquots of samples and standards were run on silica-coated thin layer chromatography (TLC) plates in either n-butanol/acetic acid/water (4 1 1 by vol. (BUOH/HAC/H2O)) or n-propanol/concentrated ammo-nia/water (8 1 11, pre-equilibration). These eluents were previously described (Keller et ah, 1984) for the two-dimensional TLC-separation of elastin cross-links. 

TLC is carried out on a 5 x 20 cm silica gel precoated plate (Merck) with a solvent system of pyridine-ethylacetate-acetic acid-water (75 25 15 30), tert-butanol-acetic acid-water (2 1 1) or n-butanol-acetic acid-water (60 15 25) (11). 

Solvent 1 = distilled water Solvent 2 = 2% aqueous acetic acid Solvent 3 = n-butanol-acetic acid-water (78 17 5). d S. Bouchilloux, Compt. Rend. Soc. Biol. 153, 1818 (1959). 

Solvent System I = n-butanol-acetic acid-water 4 1 1 Solvent System II = t-but anol-0.004N HCl-saturated NaCl solution 2 1 1 on paper impregnated with acetone-water-saturated NaCl solution 6 3 1, Ref. (149). 

Solvent System III — n-butanol-acetic acid-water 4 1 5. 

Figure 8-31. Distribution of radioactive materials after paper chromatography of a pancreatin digest of denatured yeast cells which had incorporated S-sulfate from the medium. The chromatogram was run on Whatman 3MM paper and developed in n-butanol-acetic acid-water. Individual strips 0.5 cm long were counted in a liquid scintillation counter. Met, Met-O, O, and F indicate S-methionine, S-methionine sulfoxide, origin, and solvent front, respectively. [From R. Graham and W. M. Stanley, Anal. Biochem., 47 505 (1972).]...

Mobile phases. A = n-butanol-acetic acid-water (4 1 2. v/v/v), upper ph asc. E... 

Recent studies have made it possible to classify water-organic solvent systems in CCC for separation of organic substances on the basis of the liquid-phase density difference, the solvent polarity, and other parameters from the point of view of stationary-phase retention in a CCC column [1,3-9]. Ito [1] classified some liquid systems as hydrophobic (such as heptane-water or chloroform-water), intermediate (chloroform-acetic acid-water and n-butanol-water) and hydrophilic (such as n-butanol-acetic acid-water) according to the hydrophobicity of the nonaqueous phase. Thirteen two-phase solvent systems were evaluated for relative polarity by using Reichardt s dye to measure solvachromatic shifts and using the solubility of index compounds [6]. 

After the completion of the solvent development in the first dimension, e.g., n-butanol acetic acid water (12 3 5) or pyridine acetone NH40H... 

The glycone obtained by the enzymic hydrolysis of urochloralic acid has been identifiedl97 as D-glucuronic acid by T.L.C. on a layer of SilTca Gel GF2 H, using the solvent system n-butanol/acetic acid/water (4 5 1) and visualization by charring. 

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