Eppendorf centrifuge

In both procedures purification of the nanoparticles was carried out either by means of filtration of the suspension over a 0.2 pm cellulose acetate filter or by Eppendorf centrifuging for 15 min followed by resuspension in water up to a final suspension of the nanoparticles in PBS buffer (pH 7.2) or in physiological solution. Nanoparticles loaded with a fluorescent marker... 

The cloudy reaction sample is cooled (4°C) for 15 min and then spun at 18,000 g (Eppendorf centrifuge) for 2 min to pellet the precipitated serum proteins. 

Usually DNA precipitated by the addition of ethanol is recovered by centrifuging in an Eppendorf centrifuge (Model 5412) at... 

Method 1 takes advantage of the properties of a low melting temperature agarose (Bethesda Research Laboratories, Rockville, Md., USA). The gel slice is melted at 70°C and an equal volume of 10 mM Tris-HCl, (pH 7.6), 0.1 mM-EDTA added and the solution extracted with the same volume of water-saturated phenol in one or more 1.5 ml Eppendorf centrifuge tubes. After brief centrifugation the aqueous layer is removed and the phenol extraction repeated a further 2-3 times. Finally the aqueous phase is extrac-... 

Eppendorf Centrifuge Models 5412 and 5413 Obtainable from Anderman Laboratory Supplies Division, Central Avenue, East Molesey, Surrey KT8 OQZ, U.K. 

Treat one aliquot at a time. Centrifuge in a benchtop centrifuge (Eppendorf centrifuge) for 2 s to spin down the cells. 

Allegra 25R low-speed Beckmann centrifuge or Eppendorf Centrifuge 5417R for cell recovery. 

Miniature Grinding Device. A miniature mortar and pestle can be devised by putting the sample in a 1.5-ml Eppendorf centrifuge tube and using a 0.5-ml tube to crush the sample. The lip and cap of the 0.5-ml tube can be trimmed with a razor blade to facilitate the fit of the two tubes. If the samples are to be processed with PCR amplification, it is desirable to discard each 0.5-ml tube pestle after grinding to avoid cross-contamina-... 

The reagents in phosphate-buffered saline are added, in the order stated above, to 2 ml polypropylene micro (Eppendorf) centrifuge tubes. 

In order to modify NS with 4MBA, a 10 mM solution of 4MBA in 9 1 H20/Et0H was prepared. This solution was then pipetted as a 10 pL drop on the aggregated spots described above and left at RT overnight. On the next day, the self-assembled monolayer was washed ten times with ddH20 and the slides were spun dry at 1,000 rpm for 1 min in a table top Eppendorf centrifuge. 

Eppendorf centrifuge for 30 min at maximum speed (14000 r.p.m). The crude membrane fraction (pellet) is resuspended by brief sonication in 150 1 HS supplemented with 1 % Triton XlOO and centrifuged again at 4 C for 30 min at 14000 r.p.m. The proteins (including VAMPs) extracted from the membranes during the detergent treatment are found in the supernatant. 

Transfer the samples from glass tubes to clean Eppendorf centrifuge tubes. 

Pipet 1.5 mL of the cell culture to be tested into an Eppendorf tube and centrifuge at 14,000g for 10 min in an Eppendorf centrifuge. 

While the samples are dialyzing, add 10 pL of 20% SDS to one 2.0-mL Eppendorf centrifuge tube for each well lysate, and preheat the tubes to 50°C for at least 5 min. After dialysis, transfer the cell lysates to these tubes. Add 50 pL of proteinase K (10 mg/mL in TE), mix well, and incubate the tubes for 60 min at 50°C. A white precipitate may form in the tubes on transfer of the lysates this will disappear after approx 10 min incubation at 50°C. Mix tubes again after the first 10 min of incubation to help resolubilize this SDS precipitate. 

Pipet aliquots of 50 j L of EDTA blood into four acid-washed 1500 fiL Eppendorf centrifuge tubes containing 850 nL of 0.1% Triton X-100. Flush the pipet tip 3-4 times with the Triton X-100 solution to minimize transfer error. 

Pipet into tube 1 100 of water, into tube 2 100 of standard solution 1 (2000 hqIL Pb), into tube 3 100 nL of standard solution 2 (4000 /ig/L Pb), into tube 4 100 nL of standard solution 3 (6000 ug/L Pb). The standard solutions 1 - 3 are prepared by diluting the stock solution with 0.01 M HNO3. To make a chemical blank pipet 150 nL of water and 850fiL of 0.1% Triton X-100 into an Eppendorf centrifuge tube. 

Eppendorf centrifuge (cooled at 4°C) or placed in cold room. 

After the required time, remove 4 p extract and place in a 1.5-ml Eppendorf tube and resuspend very gently in 1 ml of MWB. At this stage, centrifugation of the in vitro nuclei and organelles onto 5 mm silicon chips requires a simple modification of 1.5-ml Eppendorf centrifuge tubes as follows Remove lid from tube, and cut tube with a sharp knife at a level where the cut end fits tightly... 

Spin down the labeled RNA in an Eppendorf centrifuge (full speed) for 10-15 min at 4°C (cold room). Carefully aspirate the supernatant and discard it. 

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