Site-specific immobilization

Hoffman, W.L., and O Shannessy, D.J. (1988) Site-specific immobilization of antibodies by their oligosac-chanide moieties to new hydrazide derivatized solid supports. J. Immunol. Meth. 112, 113-120. 

Butterfield DA, Colvin J, Liu J, Wang J, Bachas L, Bhattacharrya D (2002) Anal Chim Acta 470 29 Electron paramagnetic resonance spin label titration a novel method to investigate random and site-specific immobilization of enzymes onto polymeric membranes with different properties... 

Fig. 6. The schematic representation of surface patterning, site-specific polymerization, and site-specific immobilization of microparticles.

Vigmond SJ, Iwakura M, Mizutani F et al (1994) Site-specific immobilization of molecularly engineered dihydrofolate-reductase to gold surfaces. Langmuir 10 2860-2862 Vijayendran RA, Leckband DE (2001) A quantitative assessment of heterogeneity for surface-immobilized proteins. Anal Chem 73 471 80... 

Lue, R. Y, Zhu, Q., Li, D. (2004). Site-specific peptide immobilization strategies for the rapid detection of kinase activity on microarrays. Site-specific immobilization of biotinylated proteins for protein microarray analysis. Enzymatic profiling system in a small-molecule microarray. Intein-mediated biotinylation of proteins and its application in a protein microarray. Developing site-specific immobilization strategies of peptides in a microarray. Methods Mol. Biol. 278, 191-204. 

Butterfield, D.A. Bhattacharyya, D. Daunert, S. Bachas, L. Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays a review. J. Membr. Sci. 2001,181, 29-37. 

In general, the site-specific immobilization methods described above are especially effective when the protein recognition site on ligand molecules is known or can be predicted. Small molecules immobilized on solid surfaces through a specific functional group uniformly present a certain area of their molecular surfaces, and thus the interaction with proteins that recognize this surface can be analyzed in a highly sensitive and quantitative manner. However, except in the case of certain protein-reactive small molecules [53], it is difficult to predict the... 

In this paper we report the application of the pCP technique to a functionalized alkanedisulphide - 11,11 -dithio-bis(succinimidyl-undecanoate) (DSU). We also describe a strategy for rendering etched silicon/gold structures accessible to this reactive crosslinker SAM. We subsequently present the site-specific immobilization of proteins onto such surfaces (Fig. 1) demonstrating the retained functionality of the immobilized protein. The atomic force microscope (AFM) was used to image the immobilized proteins and to determine their amount and spatial distribution on the patterned, template-stripped gold (TSG) surface. 

Ito, T., Sadamoto, R., Naruchi, K., Togame, H., Takemoto, H., Kondo, H., and Nishimura, S.-I. (2010) Highly oriented recombinant glycosyltransferases site-specific immobilization of unstable membrane proteins by using staphylococcus aureus sortase A. Biochemistry, 49, 2604-2614. 

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