Ovary, tumor

Immunohistochemistry can play an important role in the diagnosis and classification of mucinous tumors of the ovary. Two types of primary mucinous tumors occur in the ovary. Tumors composed of cells with an intestinal phenotype are most common, but a minority of mucinous tumors has an endocervical-like or seromucinous phenotype. These two types of mucinous tumors have different immunophenotypes, and immunostains can be used to differentiate between the two types. Immunostains for cytokeratin or epithelial membrane antigen can help identify foci of microinvasion. 

The cytotoxic activities of the 2, 2 -difluoro analog (775) of 737 against Chinese hamster ovary and tumor cells, in comparison with those of 1- -d-arabinofuranosylcytosine ara-C, a drug for leukemia), have been studied 775 is transported the faster through membrane into cells, more effectively phosphorylated by the deoxycytidine kinase (to the 5 -mono-phosphate) and, after conversion into the 5 -triphosphate, more highly accumulated in the cells, with longer duration time, than is ara-C, but nevertheless 775 is incorporated into the DNA to a lesser extent than is ara-C. These characteristics of 775 were discussed. 

Epithelial ovarian tumors are composed of cells that cover the surface of the ovary, such as serous, mucinous, endometrioid, clear cell, and poorly differentiated adenocarcinomas. 

Tumor involves one or both ovaries with pelvic extension... 

Tumor involves one or both ovaries with microscopic confirmed peritoneal metastasis outside pelvis and/or regional lymph node metastasis... 

Ascites or peritoneal washings Tumor on external surfaces Ovary capsule... 

Recently, Polychronopoulou45 examined tobacco, ethanol, and coffee as risk factors for ovarian cancer in Greek women in a hospital-based case-control study with data collected from 1989 to 1991. Cases were 189 women who were residents of Greater Athens and less than 75 years old with histologically confirmed common malignant epithelial tumors of the ovary. Controls were female residents of Greater Athens, less than 75... 

Generally, a CA 15-3 cutoff of 25 U/ml is used to detect stage I breast cancer. In higher stages, the sensitivity is reported to be much better, which makes it a good test of tumor burden. CA 15-3 is reported to be elevated in other disease conditions such as liver disease (particularly chronic hepatitis, cirrhosis, and carcinoma), some inflammatory conditions (sarcoidosis, tuberculosis, systemic erythematosus), and other carcinoma (lung and ovary). For this reason, positive CA 15-3 results should be interpreted with caution (20,21). 

Mammalian cell suspension cultures are the preferred choice for large-scale recombinant protein production in stirred-tank bioreactors. The most widely used systems are Chinese hamster ovary (CHO) cells and the murine myeloma fines NSO and SP2/0. In half of the biological license approvals from 1996-2000, CHO cells were used for the production of monoclonal antibodies and other recombinant glycosylated proteins, including tPA (tissue plasminogen activator) and an IgGl fusion with the tumor necrosis factor (TNF) receptor, the latter marketed as Enbrel [7]. 

Raloxifene, a more complete uterine antagonist than tamoxifen or clomiphene, significantly reduces leiomyoma size in post-menopausal women [31], yet it is less efficacious at reducing tumor volume in pre-menopausal women [32], This result has been attributed to the poor pharmacokinetic properties of this compound in which extensive conjugative metabolism of the phenol(s) limits the circulating levels of the parent drug. In addition, clinical outcomes in premenopausal women treated with raloxifene suggest that this compound, like tamoxifen and clomiphene, can affect the ovaries via the HPO axis [33]. These data, taken collectively, indicate that current SERMs lack the efficacy, pharmacokinetic, and ovarian safety properties needed to treat leiomyoma in ovulatory women. 

A second study examined the effects of DMBA initiated mi rex-promoted tumors in female mice on ovarian hormones. This study found that the loss of ovary (OVX) protected the female mice (40%) from mirex tumor promotion. Tumor promotion was unaffected in DMBA-initiated OVX mice promoted with TPA. Based on the data, the authors also concluded that there is a structural specificity in the tumor-promoting ability of mirex in mouse skin and that mirex is a much more effective skin tumor promoter in female CD-1 mice than in male CD-1 mice or OVX mice (Meyer et al. 1994). 

This digestion, isolation, and storage approach has been successfully applied to a wide range of tumor and normal tissues. Tumor tissues include both primary and metastatic tumors of the more common types, such as colon, lung, and ovarian tumors, melanomas, and sarcomas, and rare tumors, such as schwannoma. Normal cell populations include lung, ovary, colon, heart, liver, kidney, and blood. 

Tumors ICr. ( lM) Lung Ovary Kidney Normals Spleen Liver Heart Blasts... 

There was a statistically significant compound-related increase in incidence of several other tumors in female mice hemangiosarcoma of the abdominal retropehtoneum, particularly involving the area of the ovaries, uterus, kidneys, and adrenal subcutaneous fibrosarcomas and mammary adenocarcinoma (NTP 1982). A limitation of the study was poor survival in male mice from ascending suppurative urinary tract infections. 

This is an unusual drug in that it contains a metal atom, platinum (Pt) in this case. Cisplatin reacts with DNA to cross-link bases, disrupting normal DNA structure and function. This agent has found broad use in cancer chemotherapy, including efficacy in tumors of the testis, ovary, bladder, head and neck, thyroid, cervix, and endometrium. It is also active against neuroblastoma and osteogenic sarcoma. 

Vincristine resistance has been studied in Chinese hamster ovary cell lines cells resistant to vincristine also are resistant to vinblastine and vindesine. Suggestions were made that, in cells with relatively low levels of drug resistance, at least two prominent mechanisms of resistance can occur (22). In the first instance, cellular resistance may be attributable to membrane alterations that are reversible, functionally, by treatment with verapamil. In the second, resistance has been postulated to be due to an altered sensitivity of tubulin to the effects of the drugs the primary basis for postulating an altered interaction with tubulin was that a subgroup of cells resistant to vincristine showed enhanced sensitivity to taxol, a drug that can stabilize microtubules. It should be emphasized that differential sensitivities of tubulins from different tumor cells to the effects of vincristine or vinblastine has been proposed as a basis for the susceptibilities of cells to the cytotoxic effects of such drugs (23). Differences have been described in the electrophoretic patterns for tubulins obtained from vin-... 

Chronic oral exposure of rats and mice to MDA and its dihydrochloride is carcinogenic. Treatment-related increases in the incidences of thyroid follicular cell adenomas and hepatocellular neoplasms were observed in mice after chronic ingestion of MDA in drinking water. In rats, increases in the incidences of thyroid follicular cell carcinoma and hepatic nodules were observed in males and thyroid follicular cell ademonas occurred in females. Although not statistically significant, certain uncommon tumors such as bile duct adenomas, papillomas of the urinary bladder, and granulosa cell tumors of the ovary also were reported. These tumors are of low incidence in historical controls. In another report, MDA acted as a promoter of thyroid tumors in rats. °... 

Toxicological studies have suggested that the species specificity for induction of ovarian tumors (produced in mice but not rats) occurs because the blood level of the ovotoxic VCH metabolite VCH-1,2-epoxide is dramatically higher in VCH-treated female mice compared with rats. VCH has been shown to be metabolized by the liver of mice to the ovotoxic metabolite (VCH-1,2-epoxide), which circulates in blood and is delivered to the ovary, where it destroys small oocytes. This destruction of small oocytes is considered to be an early event in carcinogenesis. Species difference in epoxidation of VCH by hepatic micro-somes correlates well with the differences observed in the blood concentration of VCH-1,2-epoxide and VCH ovarian toxicity. Further in vitro studies have found that the rate of VCH epoxidation in humans by human hepatic microsomes was 13- and 2-fold lower than epoxidation by mouse and rat systems respec-tively. Therefore, if the rate of hepatic VCH epoxidation is the main factor that determines the ovotoxicity of VCH, rats may be a more appropriate animal model for humans. 

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