Fluorometric assays

Tomisek, A. J. and Natelson, S. Fluorometric assay of ultramicro quantities of glucose with Somogyi filtrate and hexokinase. Micro-chem. J. (1974),... 

Liu J, Bhalgat M, Zhang C et al (1999) Fluorescent molecular probes V a sensitive caspase-3 substrate for fluorometric assays. Bioorg Med Chem Letts 9 3231-3236... 

Rinkus SJ, Legator MS. 1985. Fluorometric assay using high-pressure liquid chromatography for the microsomal metabolism of certain substituted aliphatics to 1, Ne-ethenoadenine-forming metabolites. Anal Biochem 150 379-393. 

Ebrahim, H., and Dakshinamurti, K. (1986) A fluorometric assay for biotinidase. Anal. Biochem. 154, 282-286. 

Schreiber, A. B. and Haimovich, J. (1983) Quantitative fluorometric assay for detection and characterization of Fc receptors. Methods Enzymol. 93, 147-155. 

ELUOROACETATE/FLUOROCITRATE Fluorometric assay for avidin and biotin, BIOTIN AND DERIVATIVES Fluorophore,... 

Gibson KM, Lee CF, Chambliss KL, Kamali V, Francois B, Jaeken J, Jakobs C (1991) 4-Hy-droxybutyric aciduria application of a fluorometric assay to the determination of succinic semialdehyde dehydrogenase activity in extracts of cultured human lymphoblasts. Clin Chim Acta 196 219-222... 

Bioassay procedures for the determination of gibberellic acid have been developed (2, 5), but more recent chemical fluorometric assay methods are equally specific. However, both assay methods show a low response with samples containing less than 10 /x/xg. of the gibberellins. Consequently, in determining residual amounts within the part per billion (p.p.b.) range, relatively large samples must be extracted and extracts partially purified to satisfy the assay conditions. These operations are usually accompanied by some material losses or degradation, which impair quantitative interpretation of the results. Natural inhibitors can influence the results in the bioassay method (2), and fluorescent contaminants can interfere with the spectrophotometric analysis. 

Jacks, T. J., and Kircher, H. W., 1967, Fluorometric assay for hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone Anal. Biochem. 21 279-285. 

The discovery of Zanamivir as a potent and selective inhibitor of influenza virus sialidase prompted several researchers to investigate the synthesis and structure-activity relationship studies of Neu5Ac2en-based compounds as potential sialidase inhibitors. Exploration of these SAR studies were undertaken to optimize inhibitory activity and to improve the physicochemical properties of the sialic acid-based influenza virus sialidase inhibitor. A few in vitro assays are commonly employed to measure the effectiveness of influenza virus sialidase inhibitors. The first involves a fluorometric assay that measures release of a synthetic fluorophore following its cleavage from Neu5Ac by sialidase. Dye-uptake assay, such as the Neutral Red uptake assay, measures the uptake of a vital stain, Neutral Red in cell culture. The process requires intact membranes and active metabolism in the cell, and is expressed as percent protective rate against virus infection. The plaque-reduction assay is used to measure sialidase inhibition indirectly in cell culture, and provides some measure of the inhibitor s effect on the viability of the influenza virus. In vitro and in vivo systems for analysis of inhibitors of influenza virus enzymes have been reviewed.71... 

A simple fluorometric assay for phenytoin in plasma was reported by Dill et al3 . 

CG Knight. Fluorometric assays of proteolytic enzymes. Meth Enzymol 248 18-35, 1995. 

B Page, C Page, M Noel. A new fluorometric assay for cytotoxicity measurements in vitro. Int J Oncol 3 473-476, 1993. 

Imai, K., and Toyo oka, T. (1987). Fluorometric assay of thiols with fluorobenzoxadiazoles. In Jakoby, W. (Ed.). Methods in Enzymology Sulfur And Sulfur Amino Acids. Vol. 143, (pp.67-75). New York Academic Press. 

Rubidium exhibits a very high flux through potassium ion channels. Upon depolarization, this flux can be quantified as a measure of channel integrity (Tang et al. 2001 Chang et al. 2002). Fluorometric assays have been developed that can detect drug-induced effects on hERG channels. 

Florentin, D., Sassi, A., and Roques, B.P. 1984 A highly sensitive fluorometric assay for enkephalinase, a neutral metalloendopeptidase that releases tryrosine-glycine-glycine from enkephalins. Anal. Biochem. 141, 62-69. 

Pennise and coworkers (2002) described a continuous fluorometric assay for this enzyme for the purpose of high-throughput screens and enzyme kinetics studies. They designed an acceptor... 

Pennise, C.R. et al. 2002. A continuous fluorometric assay of myristoyl-coenzyme A protein /V-myristoyltransferase. Anal. Biochem. 300, 275-277. 

A15. Armstrong, S. G., and Dean, R. T., A sensitive fluorometric assay for protein-bound DOPA and related products of radical-mediated protein oxidation. Redox Rep. 1, 291-298 (1995). 

To investigate whether bevirimat functions as a PR inhibitor, several bioassays were conducted, including a cell-free fluorometric assay using a synthetic peptide substrate of PR and experiments using a recombinant form of the Gag precursor protein Pr55Gag. Both assays showed no effect of bevirimat on PR function compared with the PR inhibitor indinavir. Indeed, bevirimat at nanomolar concentrations could inhibit the replication of HIV-1 isolates resistant to PR inhibitors. 

Thirty-one compounds showing activity in the above assay were then replicated using MDA-U6E1 and MDA-XG4 cells. A secondary analysis for caspase-3 activity was then carried out using a CaspaseGlo Fluorometric Assay (Promega). This confirms the presence of a caspase-3-mediated death pathway, which is a traditional marker for apoptotic (as opposed to necrotic) cell death. 

The properties of protein fragments can Ik also used for the fluorometric assay of proteolytic activity. Curoffproposed that the increase in trichloroacetic acid-soluble tyrosine (both free and bound in small peptides) can be fluorometrically assayed after the condensation with nitrosonaphthol. 

Price, E. A., Coombe, D. R. and Murray, J. C. A simple fluorometric assay for quantifying the adhesion of tumor cells to endothelial monolayers. (1995). Clin. Exp. Metastasis 13,155-164. 

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