Protein of serum

Dekker A, Reitsma K, Beugeling T, Bant]es A, Fei]en J and van Aken W G 1991 Adhesion of endothelial-oells and adsorption of serum-proteins on gas plasma-treated polytetrafluoroethylene S/omaferfa/s 12 130-8... 

Hydroxymethyl-6-methyluracil (1043) was prepared many years ago from 6-methyl-uracil and formaldehyde, or in other ways. Since 1956 it has received much attention in the USSR under the (transliterated) name pentoxyl or pentoxil. It is used in several anaemic and disease conditions. For example, a mixture of folic acid and pentoxyl quickly reduces the anaemia resulting from lead poisoning pentoxyl stimulates the supply of serum protein after massive blood loss it stimulates wound healing it stimulates the immune response in typhus infection and it potentiates the action of sulfonamides in pneumococcus infections (70MI21300). 

A definitive identification of the proteins in each peak is not possible, however, the elution times of the peaks at 13-14 min. and 15 min. are close to the times which would be expected for gamma-globulins and albumins, two of the principal classes of serum proteins. These data also indicate the loading capacity of this column with serum. More than 14 mg. of undiluted serum was injected before evidence of overloading in the form of band broadening and peak distortion was observed. 

Single Sink Eq. (7.34) in the Absence of Serum Protein or Sink in Acceptor Wells... 

There are several positive factors using BSA in the basolateral medium. First, it mimics the in vivo situation where the circulating blood provides an excellent base for sink conditions due to a large volume and content of serum proteins such as albumin (serum protein content and alpha-glycoproteins) [108]. Second, serum albumin hinders adsorption onto plastic surfaces and filters and thereby stops the... 

Fig. 4 The effect of proteins on cell adhesion, (a) Kretschmann configuration for SPR. (b) Reflectance (R) as a function of incident angle (9), before (black) and after (red) the adsorption of substances, (c) Left. Time course of SPR angle shift during exposure to culture medium supplemented with 2% FBS (solid line) and the fraction of adherent cells determined by TIRFM (circles) on NH2-SAM. The dashed line is a manual fit to the symbols, included simply as a guide [42]. Right The concentrations of serum proteins in FBS...

Fig. 5 Cell adhesion to a material surface, including the adsorption of serum proteins...

We examined protein adsorption to SAMs that carried four different functional groups [42] and mixed SAMs with different wettabilities [21], Large amounts of serum proteins adsorbed to all these SAMs, but the different surface functional groups greatly affected cell adhesion behavior (Figs. 2 and 3). Thus, the amount of adsorbed proteins did not correlate with the degree of cell adhesion to SAMs. 

Cells are sometimes cultured in serum-free medium. In this condition, the surface should carry substituents of serum proteins that can directly interact with cells. SAMs of alkanethiols with bioactive ligands have been used to control interactions between the material surface and cells [80-83]. Several bioactive ligands have been tested, including RGD [80], PHSRN [81], and laminin-derived peptides [82, 83]. These ligands were expected to directly interact with cell surface integrins. 

Smithies, O., Zone electrophoresis in starch gels and its application to studies of serum proteins. Advances in Protein Chem. 14, 65 (1959). 

It is important to realize that the use of these different media for the same sample will result in separation patterns that cannot be easily compared with one another. The separation of serum proteins on cellulose acetate will result in 5-7 bands, while the use of polyacrylamide gel will give 17 bands. 

Rather than using the initial donor concentration, the final donor concentration at the termination of incubation can be used. Another approach to reduce nonspecific binding involves the addition of serum proteins [80, 120] or micelleforming excipients, such as Gelucire 44/14, Cremophor EL, or TPGS, [45] to the receiver compartment, leading to an improved assay recovery and better predictability of the model [64],... 

Ornstein21 and Davis24 developed the first high-resolution PAGE system for native proteins. Their popular system is still in widespread use. It was designed for the analysis of serum proteins, but works well for a broad range of protein types. The Ornstein-Davis buffers should be the first discontinuous system tried when working with a new, native sample. 

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