The Relative Mobilities of Serum Protein Fractions

Based upon such data, direct analysis of some fractions is possible when the components can be identified by their mobility alone. However, such an application is very limited in practice, as many subfractions have almost equal mobilities. In order to measure relative mobilities, it is practical to use pure serum albumin as reference. If the mobility of the latter is aib and the mobility of the unknown protein Ux, the quotient x/Waib is independent of the carrier medium if the migration velocity does not change during the run. In Table 3, relative mobilities 

Protein fraction Uriel (Ul) Wieme (W4) Moving boundary electrophoresis 

For quantitative evaluation of the stained fractions in the agar film, the Zeiss Extinktionsschreiber II has given us good service. 

Antibody formation after injection of heterologous immune globulin. ]. Immunol. 76, 217-227 (1956). 

Augustin, R., Fundamental aspects of single versus double diffusion methods for immunological assays. Intern, Arch. Allergy Appl. Immunol. 11, 153-169 (1957). 

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