Chromatography of Goat Serum Proteins

Blood proteins are fractionated by various procedures to give a range of purified components which may be used for a number of therapeutic or diagnostics purposes. Procedures for the fractionation of plasma proteins are adequately reviewed elsewhere [30]. Fractionation of blood proteins from a range of hosts is carried out at large-scale and in these processes ion-exchange chromatography is routinely used. In 

A = Load sample B = Buffer wash C = Gradient start 

A = Load sample B = Buffer wash C = Gradient start Fig. 6-7. Column chromatography of hen egg-white proteins on (a) DE92 and (b) DE52 using 0.025 M-Tris/HCl buffer, pH 7.5 at laboratory scale (1.5 cm i.d.xl5.5cm) at flow rates of 2mL min.  

6 Biochemical Applications of Process-Scale Ion-Exchange Liquid Chromatography 

IgG under our experimental conditions and increasing either the ionic strength of the buffer, or reducing the pH, were both effective at improving yield but also decreased the capture efficiency of non-IgG proteins thereby reducing product purity. 

---