Of 3,5-dinitrosalicylic acid

Dj potassium 3,5-Dinitrosalicylate (3,5-Dinitro-2-Hydroxybenzoic Acid, Dipotassium Salt), K2C7H207N2.H20 mw 322.33 N 8.69% OB to C02 --39.71% dark red ndles mp 280° (ignites). Sol in hot w and hot aq ethanol. Prepn is by reacting 2 moles of coned aq KOH with one mole of 3,5-dinitrosalicylic acid Hie Dipotassium salt has an impact sensy of 40cm (complete detonation) and sliding-rod test results of no detonations/all trials at angles of 45° and 75° (Ref 2)... 

Reducing-sugar reagent. This reagent is made up by mixing two solutions a solution of about 25.0 g of 3,5-dinitrosalicylic acid in 1000 mL of warm 1 A/NaOH and one of 750 g of sodium potassium tartrate, NaKC4H404 4H2O, in 750 mL of warm distilled water. These solutions are mixed slowly and diluted to a total volume of 2500 mL with warm distilled water. 

Dinitrosalicylic acid proved to be an excellent oxidizing agent for measuring very low amylase activities. The colorimetric response results from the conversion of 3,5-dinitrosalicylic acid to 3-amino-5-nitro-salicylic acid. The color produced by this reduction reaction is proportional to the actual number of reducing groups generated. 

The direct detection of a variety of simple carbohydrate compounds by u.v. methods is mentioned in reference 7 above. Spectrophotometry has also been used to determine ketoses (as furan derivatives and glucose by its reduction of 3,5-dinitrosalicylic acid to the 3-amino-5-nitro analogue. ... 

A quantum-chemical study of the mechanism of the condensation reaction between propanoic acid and aniline has been made. The mechanism of proton-transfer reactions of 3,5-dinitrosalicylic acid (41) has been the subject of a review (50 references). A successful correlation has been obtained between dissociation of a variety of acids in different solvents and quantum self-similarity measures of the CO2H fragment. The substituent effects of the isopropyl group in 2- (42), 3- (43), and 4-isopropylbenzoic acid (44) have been evaluated from their enthalpies of formation, gas-phase acidities, acidities in MeOH and in DMSO, and their IR spectra in tetrachloromethane. Particular attention was given to the influence of variable conformation on the observed steric effect. In contrast to 2-r-butylbenzoic acid and similarly to 2-methylbenzoic acid, 2-isopropylbenzoic acid exists in two planar conformations (42a,b) in equilibrium. Owing to this conformational freedom, the... 

Smith, G., U.D. Wermuth, PC. Healy, and J.M. White. 2003. Structure-making with 3,5-dinitrosalicylic acid. II. The proton-transfer compounds of 3,5-dinitrosalicylic acid with the monocyclic heteroaromatic amines. Austr. J. Chem. 56 707-713. 

The PG activity was measured [10] by detecting the increase of reducing groups during the depolymerisation of 1 % (w/v) polygalacturonic acid buffered solutions, by a colorimetric method based on the 3,5-dinitrosalicylic acid. 

High sensitivity characterizes the methods based on ion-associates formed by anionic complexes of V(V) with basic dyes. The Brilliant Green method has been described above [33]. The vanadium complex with PAR associated with Crystal Violet is extracted into a mixture of benzene with MIBK (3-f2) (e = l.l lO ) [51]. In a proposed flotation-spectrophotometric method, the V complex with 3,5-dinitropyrocatechol, associated with Rhodamine B, is separated by shaking the solution with cyclohexane the separated compound is washed and dissolved in acetone (e = 2.1-10 at 555 nm) [52], A similar sensitivity is achieved in the method using 5,7-dichloro-oxine and Rhodamine 6G [53]. Another flotation-spectrophotometric method for determining V has been based on 3,5-dinitrosalicylic acid and Rhodamine B [54]. 

Methyl ethers of D-fructose, difficult to estimate by any other means (especially those having the hydroxyl groups at C-1 and C-3 methylated), have been estimated by the alkaline 3,5-dinitrosalicylic acid reagent proposed by Bell and coworkers, and differentiation of the conformation of these methyl ethers was made possible by the addition of borate to the urea-phosphoric acid spray of Dedonder. 

A mycelial mat (2x2 mm ) was incubated in the medium for enzyme production in a 100-ml Erlenemyer flask for 5 days at 28°C using a rotary shaker. The amount of reducing sugar was measured using 3, 5-dinitrosalicylic acid. Two flasks were used for a strain. These values are average. 

Besides to 3-5-dinitrosalicylic acid, it is also used in this method the Rochelles salt (Potassium sodium tartrate), phenol, sodium bisulfite, and sodium hydroxide. The phenol optimize the quantity of the colour produced and sodium bisulfite stabilize the colour in the phenol presence (Miller, 1959). 

A review of enzyme analysers has described the automation of fixed-time and continuous-monitoring assays and the uses of partly or completely automated analysers and multi-channel systems. Automated analyses of polysaccharide hydrolases, in soluble or insoluble form, have been based on determination of the liberated reducing sugars with 3,5-dinitrosalicylic acid. A sensitive and specific method for locating glycoside hydrolases (e.g. oc-D-mannosidases) in polyacryl-... 

New sensitive assays for dextranase activity are based on the use of Sephadex substituted with either 2,4,6-trinitrophenyl groups (for spectrophotometric assay) or fluorescamine groups (for fluorometric assay). 3,5-Dinitrosalicylic acid has been used in automated assays to determine the reducing sugars released on hydrolysis of the appropriate substrates with soluble or insoluble dextranases and other polysaccharide hydrolases. ... 

Histomoniasis - In a series of substituted benzoic acid (5-nitrofurfuryli-dene hydrazides one showed outstanding activity against Histomonas meleagridis in turkeys and chickens 3, 5-dinitrosalicylic acid (6-nitro-furfurylidene) hydrazide. 

Detection was with Coomassie Blue. Lanes A and B, sample containing 2.95 mg protein/ml and 70 U/ml of a-amylase estimated by 3,5-dinitrosalicylic acid [8]. Lane C, 1 myosin (205,000), 2 3 galactosidase (116,000), 3 phosphorylase (97,000), 4 bovine serum albumine (66,000), 5 egg serum albumine (45,000), 6 carbonic anhydrase (29,000). The author thanks Dr. M. JiresovA for performing the electrophoretic analysis of a-amylase. 

Both the Fe and the FeOH " pathways appear to be important in the reaction of iron(III) with 5-chloro-, 5-nitro-, 6-hydroxy-, and 3,5-dinitrosalicylic acid but, it is suggested, only the latter features in the reactions with mandelic acid, tiron, 2,7-dichlorochromotropic acid, and citric and tartaric acids. Mechanistic analyses have also been given of the reaction of iron(III) with pyridoxamine and pyridoxal, and with thiocyanate in DMSO-water mixtures. ... 

It appears that the only anion for which the pathway via the mixed acetic salicylic anhydride is important is that of 3,5-dinitroaspirin [83]. Here the rate enhancement is not large. The rate constant (2.68 x 10 min at 39°C is only 7.3 times greater than that for the hydrolysis of the methyl ester (6.65 x 10 x 55.5 = 3.69 x 10 min ) [83] the corresponding ratio for the hydrolysis of aspirin itself is 175 [77, 79]. Hydrolysis of the anion of 3,5-dinitroaspirin in oxygen-18-enriched water leads to 39 per cent incorporation of the label into the product, 3,5-dinitrosalicylic acid, and solvolysis in 50 per cent v/v aqueous methanol yields 60 per cent of methyl 3,5-dinitrosalicylate [83]. These results can be explained only if the reaction proceeds, at least partially, via the mixed anhydride 52. Three pieces of evidence... 

To determine the concentration of inlet and outlet glucose in the ICR, a reducing chemical reagent, 3,5-dinitrosalicylic (DNS) acid 98% solution, was used. The DNS solution was... 

Apelblat, A., Manzurola, E. (1999) Solubility of o-acetylsalicylic, 4-aminosalicylic, 3,5-dinitrosalicylic, and /7-toluic acid, and magnesium-DL-aspartate in water from T - (278 to 348) K. J. Chem. Thermodyn. 21, 85-91. 

Kowkabany (28) suggests that for identification of the spots the following color reagents have the greatest general usefulness silver nitrate - ammonia, aniline hydrogen phthalate, p-anisidine hydrochloride, o-pheny-lenediamine dihydrochloride, benzidine - acetic acid, 3,5-dinitrosalicylic... 

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