Shaker, rotary

Aqueous solutions buffered to a pH of 5.2 and containing known total concentrations of Zn + are prepared. A solution containing ammonium pyrrolidinecarbodithioate (APCD) is added along with methyl isobutyl ketone (MIBK). The mixture is shaken briefly and then placed on a rotary shaker table for 30 min. At the end of the extraction period the aqueous and organic phases are separated and the concentration of zinc in the aqueous layer determined by atomic absorption. The concentration of zinc in the organic phase is determined by difference and the equilibrium constant for the extraction calculated. 

The flasks are incubated at 28°C for 48 hours on a rotary shaker with a stroke of 60 mm at 220 rpm. 2 ml of a vegetative medium thus grown are used to inoculate 300 ml Erlenmeyer flasks containing 60 ml of the following productive medium in tap water at pH 7.0. 

In addition, 1 mg of sterile S-2-hydroxyethyl-DL-homocysteine Is added to the tube and the tube is shaken on a rotary shaker at 280 cycles per minute at 25°C for seven days. 

Inoculum Preparation Stage Two batches of inoculum of about 50 gallons each are prepared by the following method A 25 ml inoculum (from the germination stage) is transferred to each of four 2-liter flasks, each containing 500 ml of the sterile medium utilized for germination. The flasks and contents are incubated for 5 days at 28°C on a rotary shaker (280 rpm, 2 inch stroke). 

The broth culture so obtained is employed as an inoculum (1%). Into each of ten flasks containing 100 ml of sterile nutrient broth is added 1 ml of the inoculum. The flasks are agitated on a rotary shaker for 8 hours at 28°C at 240 strokes per minute. After this growth period, a solution of 25 mg of 16/3-methy(cortisone in 0.5 ml of methanol is aseptically added to each flask which in turn is reshaken and incubated for an additional 24 hours. The final pH is 7.8. 

Germination stage 2 Asepticaliy transfer 25 ml of the fermentation medium of Germination stage 1 to a 2-Cshake flask containing 500 ml of theabovedescribed sterile germination medium. Incubate the flask and its contents for 3 days at 28°Con a rotary shaker (280 rpm, 2" stroke). 

Three 1 litre baffled flasks, each containing 100 ml medium, are inoculated with cells from one agar plate suspended in 10 ml saline and incubated at 30°C on a rotary shaker (for optimum supply of oxygen). This provides sufficient biomass to inoculate the bioreactor. 

The basal medium of Mandels (Mandels et al., 1976) was used with the following modifications it was buffered with 3 g/1 of sodium nitrate to pH 5.5 and supplemented with 1% w/v citrus pectin " Sigma" or other carbon sources. For enzyme production, 50 ml medium in 250 ml erlemneyer flasks were inoculatedwith spores (10 spores /ml ) exept for the non sporulating Pol 6 strain, where mycelium was used. The culture were incubated at 30° C on a rotary shaker (150 rev mn -1) for 5 days. The culture broth was filtered (Millipore 0.45 pm ) and the supernatant was analysed for pectinolytic activities, reducing sugars and proteins. 

The analytical method for difiufenican is as follows. A 50-g soil sample is extracted with 100 mL of acetonitrile for 45 min with a rotary shaker at 240 rpm. The mixture is centrifuged for 10 min at 3000 rpm, the supernatant is filtered through a glass filter funnel with anhydrous sodium sulfate and the filtrate is collected. ... 

All steps should be performed at room temperature, optimally on a gently moving rotary shaker. If the cells are very delicate (e.g., neurons) and cannot withstand gentle shaking, incubations may be performed without shaking, but times or antibody concentrations may need to be increased. 

Autoclave rotary shaker one shot cell disrupter centrifuge... 

Cultures were incubated with shaking at 250 rpm on a rotary shaker at 37 °C. A 1 % inoculum derived from 8 h stage I cultures was used to initiate fresh LB cultures (200 mL) with antibiotics in a 1 L DeLong flask. These cultures were incubated at 37 °C for 16 h with shaking at 250 rpm. 

A culture of M. isabellina CCT3498 was aseptically transferred into conical Erlenmeyer flasks (500 mL) containing 200 mL of sterile PDCB and kept on a rotary shaker (150 rpm) at 30 °C for 3 days to acquire biomass. 

Zhang and Wang (1997) studied the reaction of zero-valent iron powder and palladium-coated iron particles with trichloroethylene and PCBs. In the batch scale experiments, 50 pL of 200 pg/mL PCB-1254 in methanol was mixed with 1 ml ethanol/water solution (volume ratio = 1/9) and 0.1 g of wet iron or palladium/iron powder in a 2-mL vial. The vial was placed on a rotary shaker (30 rpm) at room temperature for 17 h. Trichloroethylene was completely dechlorinated by the nanoscale palladium/iron powders within the 17-h time period. Only partial dechlorination of PCB-1254 was observed when wet iron powder was used. 

Inoculum. Pleurotus sajor-caju was grown at 30°C on the above medium containing 1% glucose as a carbon source in 250 ml Erlynmeyer flasks on rotary shaker at 200 rpm for 60 h. The mycelial biomass thus produced was blended in a Waring blender for 1 min. under aseptic conditions in order to obtain an homogenous inoculum of well dispersed mycelial bits. The inoculum was used at the rate of 10% vol/vol. The inoculated experimental Erlynmeyer flasks were incubated at 30 C on a rotary shaker at 200 rpm for various intervals of time. 

SI 1996 No. 1342 extraction Weigh 5 g prepared sample to the nearest 0.001 g, and place in a 500-ml volumetric flask. Add 450 ml water at 20-25°C and shake for 30 min on a rotary shaker at 35-40 turns per minute. Make up to the mark with water and mix. Filter through a dry fluted filter paper into a dry container. A sample of triple superphosphate should give a solution of approximately 2000 pg P mM. 

Rotary shaker is one of suitable apparatus. Sixty minutes is usually enough to capture the specific binding proteins by affinity resins. Mixing for longer time sometimes resulted in denaturation of the active proteins in lysate and/or capture of nonspecific binding of denatured proteins. 

Figure 5. Release from alginate-encapsulated liposomes. In vitro experiments were performed as follows Encapsulated liposomes were suspended in 10 ml of physiological saline in a screw-capped vial. In cylindrical polypropylene mesh cages (mesh size 105u the mesh was obtained from Fisher Scientific, cut into 2x2.5 inch rectangles, sewn up the side with nylon thread, and sealed at the bottom with a wax plug). Release was at 37°C on a rotary shaker. The encapsulated liposomes were frequently transferred to vials containing fresh buffer in order to mimic the infinite sink conditions of the body.

After SF-treatment the rice was extracted three times, with 2 ml methanol per g rice, for 20 min on a rotary shaker. The extract was collected and evaporated to 1 ml with nitrogen and extracts analysed on HPLC. The results were calculated as pg/kg dry substance (ppb). 

The enzyme-agarose conjugate, a gel, is stored as a suspension in the immobilization buffer. This gel is rather mechanically fragile. Magnetic stirrers should be avoided, and the contents of reaction vessels gently stirred on a rotary shaker. Attention is drawn to the poisonous nature of cyanogen bromide. 

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