Neonatal mouse model

McClain RM, Keller D, Casciano D,Fu P, MacDonald J, Popp J, Sagartz J. Neonatal mouse model review of methods and results. Toxicol Pathol 2001 29(Suppl) 128-37. 

Another and more convincing explanation for this discrepancy of previous and recent studies related to the inactivation of Cryptosporidium refers to the methods of viability testing. So, it seems that in vitro viability assays (chemical excystation and vital stains) that have been used previously may have significantly underestimated the inactivation efficacy of UV-C irradiation of the parasites compared with in vivo infectivity assays applied in recent studies using neonatal mouse models (Craik et al, 2001). This was also demonstrated by UV inactivation of Giardia muris cysts using MP Hg lamps (Craik et al., 2000). 

Tang HB, DiMango E, Bryan R, Gambello M, Iglewski BH, Goldgerg JB, Prince A. Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. Infect Immun 1996 64 37-43. 

Ho, C, Conner, DA, Poliak, MR, Ladd, DJ, Kifor, O, Warren, HB, Brown, EM, Seidman, JG and Seidman, CE, 1995, A mouse model of human familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism [see comments], Nat Genet 11 389-394 Hu, J, McLarnon, SJ, Mora, S, Jiang, J, Thomas, C, Jacobson, KA and Spiegel, AM, 2005, A region in the seven-transmembrane domain of the human Ca21 receptor critical for response to Ccp-+, J Biol Chem 280 5113-5120... 

Therefore it appears that we can achieve and maintain intraocular levels of either PEDF or K1K3 angiostatin in neonatal and adult mice sufficient to expect significant reduction of retinal NV. In the ischemic mouse model the level of retinal NV is measured quantitatively by enumerating the endothelial cells above the inner limiting membrane (ILM) of the retina (see later). Such an analysis showed that PEDF treated eyes had 74% fewer endothelial cells above the ILM compared to paired controls and 78% fewer compared to paired controls for K1K3 treated eyes (Raisler et al., 2002). 

Neonatal mouse pups, obtained within less than 16 h after birth, are injected in one eye with the therapeutic viral vector. The contralateral eye serves as a control and may remain uninjected, be mock-injected with carrier solution, or be injected with a non-therapeutic vector as the experiment indicates. Having determined that mock-injection or non-therapeutic injection did not impact the experimental model, our subsequent control eyes were uninjected. 

Connolly AJ, Ishihara H, Kahn ML et al. (1996) Role of the thrombin receptor in development and evidence for a second receptor. Nature 381 516-519 Cui J, O Shea KS, Purkayastha A et al. (1996) Fatal haemorrhage and incomplete block to embryogenesis in mice lacking coagulation factor V. Nature 384 66-68 Denis C, Methia N, Frenette PS et al. (1998) A mouse model of severe von Willebrand disease defects in hemostasis and thrombosis. Proc Nad Acad Sci USA 95 9524-9529 Dewerchin M, Liang Z, Moons L et al. (2000) Blood coagulation factor X deficiency causes partial embryonic lethality and fatal neonatal bleeding in mice. Thromb Haemost 83 185-190... 

COC (2002) Committee on Carcinogenicity of Chemicals in Food, Consumer Products and the Environment (COC). COC statement on ILSI/HESI research programme on alternative cancer models. COC/02/S3, April 2002 Flammang TJ, Von Tungeln LS, Kadlubar FF, Fu PP (1997) Neonatal mouse assay for tumorigenicity. Alternative to the Chronic rodent bioassay. Reg Toxicol Pharmacol 26 230-240... 

Short- and medium-term assays in transgenic models as a basis to provide essential information about the predisposing factors to specific genetic alterations in carcinogenesis include the rat liver foci model, the XPA—/— and the p53+/— knockout mouse models, the Tg.AC and Tg.rasH2 transgenic mouse models, and Ihe neonatal... 

A neuroprotective effect of pomegranate juice was observed in a mouse model of neonatal hypoxic-ischemic brain injury in offspring of mothers administered pomegranate juice beginning at the third trimester of pregnancy... 

ACADS mutations were also point mutations, but were very rare and associated with severe neonatal forms of disease,in contrast with the mouse SCAD gene deletion mutation with missphced SCAD mRNA. Missplicing, however, may be an important mechanism to study further in the mouse model, since this has been a common finding in human patients with medium-chain acyl-CoA dehydrogenase deficiency (MCAD). ... 

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