Muscle tissue development

Targets and spirals have been observed in the CIMA/CDIMA system [13] and also in dilute flames (i.e. flames close to their lean flammability limits) in situations of enlianced heat loss [33]. In such systems, substantial fiiel is left unbumt. Spiral waves have also been implicated in the onset of cardiac arrhytlnnia [32] the nomial contractive events occurring across the atria in the mannnalian heart are, in some sense, equivalent to a wave pulse initiated from the sino-atrial node, which acts as a pacemaker. If this pulse becomes fragmented, perhaps by passing over a region of heart muscle tissue of lower excitability, then spiral structures (in 3D, these are scroll waves) or re-entrant waves may develop. These have the incorrect... 

Kim BS, Nikolovski J, Bonadio J, et al. CycUc mechanical strain regulates the development of engineered smooth muscle tissue. Nature Biotech, 1999, 17, 979-983. 

PBPK models have also been used to explain the rate of excretion of inhaled trichloroethylene and its major metabolites (Bogen 1988 Fisher et al. 1989, 1990, 1991 Ikeda et al. 1972 Ramsey and Anderson 1984 Sato et al. 1977). One model was based on the results of trichloroethylene inhalation studies using volunteers who inhaled 100 ppm trichloroethylene for 4 horns (Sato et al. 1977). The model used first-order kinetics to describe the major metabolic pathways for trichloroethylene in vessel-rich tissues (brain, liver, kidney), low perfused muscle tissue, and poorly perfused fat tissue and assumed that the compartments were at equilibrium. A value of 104 L/hour for whole-body metabolic clearance of trichloroethylene was predicted. Another PBPK model was developed to fit human metabolism data to urinary metabolites measured in chronically exposed workers (Bogen 1988). This model assumed that pulmonary uptake is continuous, so that the alveolar concentration is in equilibrium with that in the blood and all tissue compartments, and was an expansion of a model developed to predict the behavior of styrene (another volatile organic compound) in four tissue groups (Ramsey and Andersen 1984). 

In the United States, the threshold mercury concentration for commercial sale of fish is determined by the Food and Drag Administration, whereas consumption advice for recreational (noncommercial) fish is developed by individual states and tribes. Mercury data collected for development of fish-consumption advisories are typically from analyses of filets (axial muscle tissue, with or without skin) for total mercury, with concentrations expressed on a wet-weight basis. Analysis of filets for total mercury yields a valid estimate of MeHg concentration (Grieb et al. 1990 Bloom 1992), whether the analyzed sample consists of a large filet or a small mass of tissue obtained with a biopsy needle (Cizdziel et al. 2002 Baker et al. 2004). 

Various antibody preparations have been developed that facilitate imaging of vascular-related conditions, including myocardial infarction, deep vein thrombosis and atherosclerosis. Anti-myosin monoclonal antibody fragments (Fab) labelled with mIn, for example, have been used for imaging purposes in conjunction with a planar gamma camera. The antibody displays specificity for intracellular cardiac myosin, which is exposed only upon death of heart muscle tissue induced by a myocardial infarction (heart attack). 

As we grow older our muscle strength diminishes and the risk of developing sarcopenia increases. The meaning of the word sarcopenia is an abnormal decline in muscle strength and mass. Another word is muscle atrophy. Between early middle age and older age the mean decrease is 50% of muscle mass. Another way to calculate the loss of muscle mass is that over 50 years of age 1-2% of muscle tissue mass vanishes yearly. Between 50 and 70 years of age almost 15% of muscle strength per 10 years disappears. The resulting disability in older persons with sarcopenia has been calculated to cost approximately 900 dollars per person and year. The yearly total of healthcare expenditures for sarcopenia in the United States is estimated at 18-20 billions (Janssen et al. 2004). 

A subsequent study in 2002 of 27 families with a condition known as multiminicore disease (MmD) also linked mutations in SEPNl to disease pathology. Multiple mutations were identified in exons 1, 5, 7, 8, 10, and 11, and the authors also mentioned that this region (RSMD) had been previously linked to MmD. Minicores are lesions by histochemistry of mitochondrial depletion within muscle tissue. The first biochemical study of selenoprotein N aimed to identify the protein localization by immunohistochemistry and found that the primary protein product of several identified mRNAs (splice variants) was a 70 kDa protein present in the endoplasmic reticulum. Two potential ER targeting domains were shown to be present and the peptide expressed from the first exon was shown to be required for localization into the ER. This study also revealed that selenoprotein N was an integral membrane protein that is N-glycosylated. Expression analysis showed pronounced levels in embryonic tissue with a reduction after development and differentiation. 

Nandrolone facilitates formation of body muscle mass and strengthens the process of osseous tissue development. The main indications for using nandrolone, as well as other anabolic steroids, are abnormal protein anabolism, asthenia, diseases accompanied by protein loss, adrenal insufficiency, steroid diabetes, and prolonged condition of sluggishness. Synonyms of this drug used in the form of acid esters are retabolil, fenobolin, eubolin, and many others. 

The impact of formulation on protein absorption and disposition is also an important factor in the development and use of biologic molecules. Stability of the protein drug in subcutaneous or muscle tissues and absorption rates directly influence the overall response. Various physical and chemical approaches are used to stabilize proteins and other macromolecules as a part of optimizing dosage formulations. 

Although nittate was the traditional meat curing salt, Haldane (1901) demonstrated cured meat pigment development by addition of nitrite to hemoglobin. Hoagland (1908) concluded that bacterial or muscle tissue reduction of nitrate... 

Immunoaffinity cleanup was first applied in drug residue analysis for the determination of chloramphenicol in swine muscle tissue by LC (113). The lAC column was prepared using monoclonal antibodies originally developed for an enzyme-linked immunosorbent assay (ELISA) method (171) specific for chloramphenicol. Meat samples were extracted with water, and a concentrated phosphate buffer was added to the filtered extracts before immunoaffinity cleanup. A phosphate buffer was used in the washing process, whereas chloramphenicol was eluted from the column with a glycine/sodium chloride solution of pH 2.8. For subsequent LC analysis, this eluate was extracted with ethyl acetate, evaporated, and reconstimted in the mobile phase. The same analytical scheme was later successfully applied for the determination of chloramphenicol in eggs and milk as well (170, 172). 

Since 1974, Bacillus subtilis EGA has been officially employed as the test organism in the German Hemmstoff test to detect residues of tetracyclines, -lactams and aminoglycosides in kidney and muscle tissues with high sensitivity (72). Macrolides can be also detected, but to a lesser extent, whereas chloramphenicol and sulfonamides are difficult to detect. For better detection of sulfonamides, a modification of this test, the German three-plate inhibition test, was developed. This test is based on the same test organism but uses three pH values (6, 8, and 7.2), with the addition of trimethoprim. The pH relationship between the three... 

Apart from radioimmunoassays, various enzyme-linked immunosorbent assays have been described as well. Campbell et al. (42) first reported a sensitive and specific ELISA using polystyrene tubes and a polyclonal antibody. However, the performance of this method was not evaluated with real samples but only with standards and aqueous muscle tissue extracts. Sensitive ELISAs were also developed for the determination of chloramphenicol in milk (43) and eggs (44) the results drawn by the latter assay correlated well with those obtained by application of a radioimmunoassay. 

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