German Hemmstoff test

Since 1974, Bacillus subtilis EGA has been officially employed as the test organism in the German Hemmstoff test to detect residues of tetracyclines, -lactams and aminoglycosides in kidney and muscle tissues with high sensitivity (72). Macrolides can be also detected, but to a lesser extent, whereas chloramphenicol and sulfonamides are difficult to detect. For better detection of sulfonamides, a modification of this test, the German three-plate inhibition test, was developed. This test is based on the same test organism but uses three pH values (6, 8, and 7.2), with the addition of trimethoprim. The pH relationship between the three... 

The four-plate test was initially based on the German Hemmstoff-test with an additional plate of Sarcina lulea at pH 8.0, designed for the detection of lower levels of macrolides, and a fourth plate of Escherichia coli at pH 7.2 for the detection of sulfonamides (74,75). The modified version adopted by the European Community for screening carcasses is based on three plates with Bacillus subtilis BGA at pH values of 6.0, 8.0, and 7.2 with added trimethoprim, respectively, and a fourth plate with Micrococcus luteus NCTC 8340 at pH 8.0 (74). This test as described elsewhere (76) is intended to detect residues of -lactams, tetracyclines, aminoglycosides, sulfonamides, and macrolides in muscle tissue of slaughtered animals, without any prior extraction or cleanup. 

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See also in sourсe #XX -- [ ]

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