Monoclonal antibodies Fab fragments

Jacobo-MoUna, A., Qark, A. D., Jr., Williams, R. L., Nanni, R. G., Clark, P, Ferris, A. L., Hughes, S. H. and Arnold, E. (1991). Crystals of a ternary complex of human immunodeficiency virus type 1 reverse transcriptase with a monoclonal antibody Fab fragment and double-stranded DNA diffract X-rays to 3.5- A resolution. Proc. Natl. Acad. Sci. USA 88,10895-10899. 

Gratz S, Raddatz D, Hagenah G, Behr TM, Behe M, Becker W (2000) " Tc-labelled antigranulocyte monoclonal antibody FAB fragments versus echocardiography in the diagnosis of subacute infective endocarditis. Int J Cardiol 75 75-84... 

Tachibana H, Cheng X J, Watanabe K, et al. (1999). Preparation of recombinant human monoclonal antibody Fab fragments specific for Entamoeba histolytica. Clin. Diagn. Lab. Immunol. 6 383-387. 

SchmaljohnC, Cui Y, Kerby S,et al. (1999). Production and characterization of human monoclonal antibody Fab fragments to vaccinia virus from a phage-display combinatorial library. Virol. 258 189-200. 

Adamczyk, M. Grote, J. Moore, J. A. Rege, S. D. Yu, Z. Structure-binding relationships for the interaction between a vancomycin monoclonal antibody Fab fragment and a library of vancomycin analogs and tracers. Bioconjugate Chem. 1999, 10, 176-185. 

Laver, W. G., Thompson, S. D., Murti, K. G., and Portner, A., 1989, Crystallization of Sendai virus HN protein complexed with monoclonal antibody Fab fragments. Virology 171 291-293. 

CEA-scan (Arcitumomab, murine monoclonal antibody (Mab) fragment (Fab), directed against human carcinoembryonic antigen, CEA)... 

In chimpanzees, administration of Fab fragments of a monoclonal anti-F-VII antibody preceding an endotoxin bolus injection effectively blocked the activation of the coagulation pathway (B25). Administration of monoclonal anti-lL-6 under the same experimental conditions attenuated the activation of coagulation, while the fibrinolytic system remained unaltered. However, administration of monoclonal anti-TNF enhanced the tendency to microvascular thrombosis (P17,18). Monoclonal anti-TF antibodies administered to baboons as a pretreatment attenuated coagulopathy after induction of E. coli sepsis in these animals (T4). Primates pretreated with anti-C5a antibodies before infusion of E. coli developed less hypotension and had better survival rates than untreated animals, who developed ARDS and septic shock with a mortality rate of 75% (S35, Z6). No favorable treatment results have been published yet with one of these treatment modalities given to humans. 

Ghetie, V., Ghetie, M.-A., Uhr, J.W., and Vitetta, E.S. (1988) Large scale preparation of immunotoxins constructed with the Fab fragment of IgGl murine monoclonal antibodies and chemically deglyco-sylated ricin A chain./. Immunol. Meth. 112, 267-277. 

Various antibody preparations have been developed that facilitate imaging of vascular-related conditions, including myocardial infarction, deep vein thrombosis and atherosclerosis. Anti-myosin monoclonal antibody fragments (Fab) labelled with mIn, for example, have been used for imaging purposes in conjunction with a planar gamma camera. The antibody displays specificity for intracellular cardiac myosin, which is exposed only upon death of heart muscle tissue induced by a myocardial infarction (heart attack). 

Incubate specific mouse monoclonal primary antibody with FITC -conjugated mouse IgG specific Fab fragments at a ratio of 1 2 (weight for weight, based on concentration data supplied by manufacturers) in a small volume (e.g., in 10 pi or more, typically 1 pg of primary antibody in 10 pi) of staining buffer in a microcentrifuge tube for 20 30 min at room temperature. 

The ratio of primary antibodies to Fab fragments required for the formation of complexes that produce optimal immunolabeling of specific antigen does not appear to vary dramatically with primary antibody specificity or species. Primary antibody to Fab fragment ratios of 1 2 1 4 (weight for weight, based on concentration data supplied by manufacturers) typically produces optimal results with primary mouse monoclonal antibodies. 

Fig. 9.1 Immunolocalization of Adenosine Receptor A (FITC, green) in mouse myocardium using mouse monoclonal primary antibody after blocking mouse endogenous immunoglobulins by preincubation with unconjugated Fab fragment Goat Antimouse IgG. Red color accounts for cardiomyocytes and erythrocytes autofluorescence captured under illumination with a filter excit ing the autofluorescence in red spectrum. Nuclei are counterstained with DAPI (blue). Courtesy of Stephanie Grote...

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