Morris hepatoma

Josic, Dj., Hofmann, W., Wieland, B., Nuck, R., and Reutter, W., Anion-ex-change high-performance liquid chromatography of membrane proteins from liver and Morris hepatomas, /. Chromatogr., 359, 315, 1986. 

J. P. MacManus, D. C. Watson, and M. Yaguchi, The complete amino acid sequence of oncomodulin—a parvalbumin-like calcium-binding protein from Morris hepatoma 5123tc, Eur. J. Biochem. 136, 9-17 (1983). 

Ring substitution allowed us to alter the pKa and 6-fluoropyridoxamine (6-FPAM) offered superior characteristics with pKg 7.05 [291]. As for 6-FPOL, we have observed intra- and extracellular signals in whole blood and perfused rat hearts [11,291] and in addition, specific tumor cells (Morris hepatoma... 

The value of A app is tumor-dependent. It varies from 3.1 xlO 8 cm2/cmH20/sec in Morris hepatoma 5123 (Swabb et al., 1974) to 1.8xl0-6cm2/cmH2O/sec in a murine mammary carcinoma (MCalV) (Netti et al., 2000). The variation is likely due to the difference in tumor lines rather than experimental methods, because the same methods have also been used to quantify A app in a human colon adenocarcinoma (LS174T) in three different studies and the data from these studies are nearly identical (Boucher et al., 1998 Netti et al., 2000 Znati, 1995). 

The problems of identification are not nearly so acute when dealing with metalloproteins as they have a wide range of g values, and a number of these have been observed in whole tissue. Thus, the low spin ferric form of cytochrome P-450 has been observed in liver and its concentration found to be reduced in Morris hepatomas [82]. Adrenal ferredoxin in adrenal glands [83], sulphite oxidase in liver [84], iron-sulphur proteins in pigeon heart [85], methaemoglobin and erythrocyte catalase in whole blood are further examples in mammals. 

The possible presence of transfer protein inhibitors in cytosol may require the quantitation of transfer proteins by techniques that do not rely on traditional transfer assays. Teerlink et al. (1981) used a doubleantibody radioimmunoassay specific for the phosphatidylcholine transfer protein in rat liver. The assay was effective over a range of 4 to 50 ng of transfer protein. The levels of phosphatidylcholine transfer protein in three lines of Morris hepatomas was found to be in agreement when quantitated by radioimmunoassay (Teerlink et al., 1981) or immunoti-tration (Poorthuis et al., 1980). In normal liver cells, the amount of phosphatidylcholine transfer protein measured with a transfer activity assay was only about one-third of the same protein measured by radioimmunoassay. Apparently not all of the transfer protein with immuno-reactivity possesses transfer activity. Possibly, modulators of transfer activity are present in normal rat liver. 

Fukuda K, Kojiro M, Chiu J-F (1993) Demonstration of extensive chromatin cleavage in transplanted morris hepatoma 7777 tissue apotosis or necrosis Am J Pathol 142 935-946... 

Criss, W. E., Murad, F., Kimura, H., and Morris, H. P. (1976). Properties of guanylate cyclase in adult rat liver and several Morris hepatomas. Biochim. Biophys. Acta 445, 500-508. 

As in isolated mitochondria, the permeability transition pore was opened in mitochondria in Morris hepatoma ICl cells, leading to depolarization [49]. Adverse effects on mitochondrial metabolism by yessotoxin appear unlikely, however since this substance, even at micromolar concentrations, did not inhibit formazan formation from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium in rat glioma C6 cells [55], a process that requires the presence of functional mitochondrial reductases [56]. Yessotoxin did not cause membranal depolarization in the BE(2)-M17 human neuroblastoma cell line [57]. 

Hostetler, K. Y., Zenner, B.D., and Morris, H.P., Phospholipid content of mitochondrial and microsomal membranes from Morris hepatomas of varying growth rates. Cancer Res., 39 (8), 2978,1979. 

H34 Hoffman, D. H. and Sweeney, M. J. Orotate phosphor-ibosyl transferase and orotidylic acid decarboxylase activities in liver and Morris hepatomas. Cancer Res., 33, 1109-1112 (1973)... 

Fig. 2. (right) Effect of poly(ADP-ribose) polymerase on transcription of cloned rat rDNA in unfractionated whole cell extract and unfractionated nuclear extract derived from Morris hepatoma 3924A. Whole cell and nuclear extracts were prepared as described (1). HS-C was added at two different concentrations and Xho I-cleaved rDNA was used as a template in a run-off assay (Fig. 1). Lanes 1, 2 and 3 correspond to transcription in 18 lg unfractionated hepatoma nuclear extracts in the presence of 45 ng and 90 ng of HS-C, respectively. Lanes 4, 5 and 6 represent transcription in unfractionated whole cell extract (18 ng) in the absence or in the presence of 45 ng and 90 ng of HS-C, respectively.

Fig. 4. Induction of peroxisomal fatty acid oxidation by fatty acids in hepatoma cells in vitro. Morris hepatoma cells 7800 Ci [19] were grown in Ham s F 10 medium with 10% horse serum and 3% calf serum in the absence or presence of 1 mM fatty acid for 3 days (two parallels)...

Figure 13 Purification of monospecific rabbit antibody against annexin VI. The rabbit polyclonal antibody against rat annexin Iv (CCBP 65/67) reacts against other annexins (CBP 35 and CBP 33). Monospecific polyclonal antibodies against annexin VI were prepared by immobilized antigen affinity chromatography. Purified annexin VI is immobilized on an epoxy activated CIM disk (BIA Separations) and 1 mL of antiserum is diluted 1 5 in PBS and applied to the disk (flow rate 3mL/min). Bound antibodies were eluted by 0.1 M glycine HCI, pH 3.0, in a step gradient. The Western blot analysis of EDTA extracts of Morris hepatoma rat plasma membranes with the antiserum before and after the affinity purification is shown.

HTC cells (designated HTC for hepatoma tissue culture) were derived from the ascites form of a rat-carried Morris hepatoma 7288 C (Thompson et al, 1966). Previous studies have revealed that cells of this kind are able to desaturate and elongate fatty acids In this respect it was demonstrated that culture HTC cells preserved the ability to desaturate stearic to oleic acid ( A9 desaturase), a-linolenic acid to octdeca-6,9,12,15-tetraenoic acid (A6 desaturase), and eicosa-8,ll,14-trienoic acid to arachidonic acid (A5 desaturase) (Alaniz et al, 1975). They are also able to convert a-linolenic acid to higher homologs with 5 and 6 double bonds by desaturation and elongation reactions. These results also proved the existence of A4 desaturase activity (Alaniz et al, 1975). 

L-Fucoprotein metabolism in Morris hepatoma 7777 plasma membranes is characteristically different from that in normal liver and host liver membranes. The membrane glycoproteins of Zajdela s hepatoma cells have been shown to contain D-galactosyl- but not 2-acetamido-2-deoxy-D-galactosyl-residues in the non-reducing terminal positions or in positions penultimate to neuraminic acid. ... 

Lea and Randolph (2001) also examined the induction of histone acetylation in tumor-bearing rats. Increased acetylation of histones in liver and Morris hepatoma 7777 was induced by treatment of rats with DADS (200 mg/kg b.w.), and allyl mercaptan (100 mg/ kg b.w.). The level of histone acetylation was greater in liver than in the hepatoma, and the response to the organosulfur compounds tended to be weakened in the tumor. Thus the compoimds in garlic or their metabolites may increase the acetylation of core nucleoso-mal histones for the induction of differentiation. 

Hudgin et al. (1971) have compared sialyl- and N-acetylglucosa-minyltransferase levels in three types of Morris hepatoma decreased sia-lyltransferase levels were found in the more rapidly growing tumors. Since these transferases are located in the Golgi apparatus (see Section IV,A), it appears that the Golgi apparatus may be undergoing alterations in function in these cells. 

MORRIS HEPATOMAS Mechanisms of Regulation Edited by Harold P. Morris and Wayne E. Criss... 

The amounts of haematosides, monosialogangliosides, and disialogangliosides extracted from the blood sera of Morris hepatoma-bearing rats are higher than those extracted from the sera of normal rats, whereas the content of trisialo-... 

CMP-Neu5Ac moderate increase Morris hepatoma 7777, 3924 A Harms et al. 1973, Reutter and Bauer 1978, Grunholz 1978... 

UDP-N-Acetylgluco-samine-2 -epimerase strong decrease Morris hepatomas Yoshida sarcoma, Yoshida ascites tumor Reutter et al. 1970, Kikuchi et al. 1971, Harms et al. 1973, Grunholz 1978 Kikuchi (2/. 1971... 

N-Acetylmannosamine-kinase decrease Morris hepatomas, Yoshida sarcoma, Yoshida ascites hepatoma Kikuchi et al. 1971, Grunholz 1978... 

CMP-Neu5Ac hydrolase decrease Morris hepatoma 7777, 3924 A ovarian tumors (human) Grunholz 1978 Chatterjee et al. 1978... 

Morris hepatoma 7777, 7800 thyroid tumor (rat) ascites hepatoma cells Morris hepatoma 96I8A2, 3924A... 

Unchanged Morris hepatoma 5123 D colon carcinoma (human)... 

---