Mouse salivary gland

In humans, the structural gene locus is on chromosome 19 (M17), and the gene spans over 40 kilobases (kb) including 18 exons and 17 introns (W2, X2). Neu-roleukin, a protein that acts as both a neurotrophic factor and a lymphokine, has been isolated from mouse salivary glands (G7), and subsequently the primary structure of neuroleukin was found to be identical to that of GPI by comparison of the cDNA sequences (C7, FI). The cDNA sequence encodes 558 amino acid residues. The enzyme consists of two identical subunits with a molecular weight of approximately 63,000 and neuroleukin is active as a monomer. 

WiGLEY, C.B. (1979). "Hansformation in vitro of adult mouse salivary gland epithelium a system for studies on mechanisms of initiation and promotion, page 3 in Neoplastic Thinsformation in Differentiated Epithelial Cell Systems In Vitro, Franks, L.M. and Wigley, C.B., Eds. (Academic Press, London). 

Nerve growth factor (NGF) is a polypeptide, a molecule composed of several amino acid units that has a protein-like behavior, but is not as complex as a protein in structure. NGF increases the growth of nerve cells, especially those in the peripheral nervous system, and directs the growth and orientation of nerve cell axons (processes which carry impulses away from the nerve cell body to adjoining dendrites). NGF is one of many growth factors found to be essential in cell division (mitosis), and has been isolated from a variety of cells such as mouse salivary glands and developing nerve tissue. The behavior of NGF resembles that of polypeptide hormones such as insnlin. 

The results from Compound I led to the conclusion that since the R S potency ratio was the same in vitro and in vivo and in both central and peripheral potency tests, the anticholinergic receptor must be the same in the guinea pig ileum, the mouse eye, the mouse salivary gland and in the CNS. With regard to studies of behavioural effects, the implication of this conclusion is that if the behavioural effects of anticholinergic drugs are directly related to their anticholinergic properties then the ratio... 

Although the nerve growth-promoting protein was first purified from the mouse salivary gland by Cohen (1960), it took more than a decade to determine the amino acid sequence of NGF (Angeletti et al., 1973) and another 10 years to achieve the first full gene sequence analysis (Scott et al., 1983 Ullrich et al., 1983). 

Aloe, L., Alieva, E., Bohm, A. and Levi-Montalcini, R. (1986) Aggressive behaviour induces release of nerve growth factor from mouse salivary gland in the bloodstream. Proc. Natl. Acad. Sci. USA 83 6184-6187. 

Cohen, S. (1960) Purification of a nerve growth promoting protein from the mouse salivary gland and its neurocyto-toxic anti-serum. Proc. Natl. Acad. Sci. USA 46 302-311. 

Epidermal growth factor mouse salivary gland). Murine epidermal growth factor. ct-Epidermal growth factor mouse salivary gland reduced) cyclic 6 20), 14- 31), 33 42)-tris disulfide)... 

Epidermal growth factor (mouse salivary gland), see M-30140... 

In the periodic acid/phenylhydrazine/Schiff (PAPS) procedure of Spicer (1961) a treatment with phenylhydrazine is interposed between the periodic acid oxidation and Schiff reagent steps of the standard PAS. As phenylhydrazine reacts readily with aldehydes it would be expected to block all subsequent reaction with Schiff reagent. It was found, however, that when this sequence was employed with sections of mouse salivary glands and other tissues rich in sialomucins, PAS staining was reduced but not abolished. When, however, the salivary glands were pre-treated with a neuraminidase the positive Schiff staining in the PAPS... 

NGF isolated from Naja naja venom has a high degree of amino acid sequence homology to mouse NGF. NGF isolated from mouse salivary gland has three subunits-biologically active subunit is the p-unit. However, snake venom NGF does not have aPy complexes. The similarity between the amino acid sequences of NGF from two sources suggests that the active sites of NGF are highly conserved. 

Mouse i.p. Lung (Adenoma, Adeno carcinoma) Salivary glands ( ) 29,47,48 29... 

Another important source of variation in enzymes as well as in other proteins is alternative splicing of mRNA.33 For example, transcription of the mouse a-amylase gene in the salivary gland starts at a different site (promoter) than does transcription in the liver. 

Fig. 4. Sexual dimorphism in the mouse submaxillary salivary gland.

Hamilton PRP-1 polystyrene cartridge (10 cm x 4.6 mm) or column of Ultrasphere ODS. 20 mM Tris-acetate (pH 7) 200 mM in octano-sulfonate and containing 2.5% of acetonitrile [2mL/min]. Electrochemical Rat or mouse brain or salivary gland tissues [149]... 

Stockelman et al. (1998) described chronic renal failure in a mouse model of human adenine phospho-ribo-syltransferase deficiency. Hamilton and Cotes (1994) used a partial nephrectomy model in mice with two-thirds of the total renal mass excised to evaluate erythropoiesis and erythropoietin production from extrarenal sources such as the submandibulary salivary gland. Koumegawa et al. (1991) suggested the DBA/2FG-pcy mouse, which develops numerous cysts in kidney cortex and medulla, a progressive anemia and an elevation of blood urea nitrogen, as a useful spontaneous model of progressive renal failure. 

Tsuneyama K, Van dWJ, Naltanuma Y, Cha S, Ansari A, Coppel R, et ai. Human combinatorial autoantibodies and mouse monoclonal antibodies to PDC-E2 produce abnormal apical staining of salivary glands in patients with coexistent primary biliary cirrhosis and Sjogren s syndrome. Hepatology 1994 20 893-8. 

No Independent confirmations of these findings have appeared. Two studies, however, are consistent with the Egyhazi and Hyden data. Jacob and Sirlinl 6 found pronounced elevations ija vitro in SNA concentrations in the salivary glands of an insect, and Essman O reported elevated total SNA concentrations in several parts of mouse brains after TCAP treatment. 

---