Tris-acetate

New water-insol. naphthalic acid imide dyestuffs - used to dye blends of polyamide or urethane- and polyester or tri acetate fibres having good light and washing fastness C91-110.342 RICH DE GB LI) ... 

L-Cysteine/Cyanide Cyanoalaninesynthase Cyanoalanine, HS Tris-Acetate pH 8.5 Ag2S ... 

Stellwagen, NC, Apparent Pore Size of Polyacrylamide Gels Comparison of Gels Cast and Run in Tris-acetate-EDTA and Tris-borate-EDTA Buffers, Electrophoresis 19, 1542, 1998. Stellwagen, NC Gelfi, C Righetti, PG, The Free Solution Mobility of DNA, Biopolymers 42, 687, 1997. 

Acetate A general name for processes for making cellulose acetate fibers. Cellulose is acetylated, dissolved in acetone, and spun into fibers by injecting through orifices into heated chambers. Cellulose mono-acetate is made by acetylating with a mixture of acetic acid, acetic anhydride, and sulfuric acid as the catalyst. Cellulose tri-acetate is made in a similar fashion, but using perchloric acid as the catalyst, and dry-spinning from a solution in ethanol/ methylene chloride. Cellulose tri-acetate fibers were first made commercially by Courtaulds in London in 1950. 

Other buffers that have been used for continuous, native electrophoresis are Tris-glycine (pH range 8.3 to 9.5),19 Tris-borate (pH range 8.3 to 9.3),26 and Tris-acetate (pH range 7.2 to 8.5).27 Borate ions26 can form complexes with some sugars and can therefore influence resolution of some glycoproteins. 

In many cases, the analytical tasks are simply to detect and quantify a specific known analyte. Examples include the detection and quantification of commonly used buffer components (e.g., Tris, acetate, citrate, MES, propylene glycol, etc.). These simple tasks can readily be accomplished by using a standard one-dimensional NMR method. In other situations, the analytical tasks may involve identifying unknown compounds. This type of task usually requires homonuclear and heteronuclear two-dimensional NMR experiments, such as COSY, TOCSY, NOESY, HSQC, HMBC, etc. The identification of unknown molecules may also require additional information from other analytical methods, such as mass spectrometry, UV-Vis spectroscopy, and IR spectroscopy.14... 

Hollosep High Rejection Type is characterized by Cellulose Tri Acetate (CTA) hollow fiber with dense membrane structure and high salt rejection, and also by the module configuration favorable for uniform flow of feed water through hollow fiber layers (5 ). These features suggest that Hollosep may be operated under the conditions of higher recovery ratio compared to conventional conditions. 

Preparation of Hollow Fiber Membrane. CTA (Cellulose Tri-Acetate) hollow fiber membranes were prepared by aplnning a dope solution of CTA followed by soaking and anealing. 

These kinetic experiments were carried out in microcuvettes at 25°C, which contained 25 nmoi reduced pyridine nucieotide, 50 nmoi potassium ferricyanide (added at zero time as the eiectron acceptor), 1 nmoi reductase in 0.2 ml 0.1 M Tris-acetate (pH 8.1) containing 1 mM EDTA. 

Fig. 6. Apoptotic DNA ladder pattern of eosinophils treated with dexamethasone (Dexa, 2 (xM) for 18 h (Zl). DNA was extracted from cells with ethanol (P4) and electrophoresed on 1% agarose gel in 1 X TAE (Tris acetate-EDTA) buffer (pH 8.0). After electrophoresis, the gel was soaked in 1 x TAE buffer containing 0.5 /tg/ml ethidium bromide, and DNA was visualized by an ultraviolet illuminator. Reproduced with permission from Zhang, J. R, Wong, C. K., Lam, C. W. K., Ho, C. Y., and Hjelm, N. M., Biochemical assessment of apoptosis. Chinese J. Lab. Med. Clin. Sci. 1, 27-28 (2000).

Aparose el for DNA electrophoresis. Agarose is suspended in TAB buffer (40 mM Tris-acetate [pH 8.3], 1 mM EDTA) and dissolved by heating in a microwave oven. When the temperature drops to around 65°C, pour it into trays and allow for gelation. 

Tris-Acetate-EDTA buffer (50x) [TAE Buffer] pH 8.3 (Nacalai Tesque) Store at room temperature. 

Standard research-grade agarose is usually sufficient, but special agaroses may be used for specific applications (e.g. high-resolution gels). The electrophoresis buffer is usually prepared and stored as a 10 x concentrated stock. The most commonly used buffer is Tris-borate-EDTA (TBE), or alternatively, one may use Tris-acetate-EDTA (TAE) buffer ... 

C, 20mM Tris-acetate buffer, pH 8.0, ImM dithiothreitol, 5% glycerol, several months [1]... 

Tri-acetate hollow fibers Polyamide hollow fibers Cellulose acetate spiral-wound... 

Buffers appropriate for electrophoresis gels include Tris-glycine, Tris-acetate, Tris-phosphate, and Tris-borate at concentrations of about 0.05 M. 

Agarose slurry buffer, 0.04 M Tris-acetate, 0.002 M EDTA, pH 7.8 Ethidium bromide solution, 10 mg/mL in H20... 

Electrophoresis buffer, Tris-acetate buffer listed above containing ethidium bromide, 0.5 p,g/mL... 

Gel-loading buffer, 0.04 M Tris-acetate containing 50% glycerol and 0.25% bromophenol blue tracking dye, pH 8.0. 

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