Murine epidermal growth factor

Akimoto T, Hunter NR, Buchmiller L, et al. Inverse relationship between epidermal growth factor receptor expression and radiocurability of murine carcinomas. Clin Cancer Res 1999 5 2884-2890. 

Murine epidermal growth factor is a polypeptide containing 53 residues. Its molar mass is 6040 g/mol. Chromatography on diethylaminoethyl cellulose produced a single peak. The isocratic RPC of this seemingly homogeneous material on a C 18 column (300 x 7.8 mm) yielded two clearly separated peaks (Fig. 19)77). The eluent was water — acetonitrile (74 26), 0.04 M in triethylamine acetate, pH = 5.6. The structure of the p component is identical with that of the a component but does not possess the Asn residue at chain position 1. 

Fig. 19. Efficiency of RPC in protein separation. Isocratic RPC separation of murine epidermal growth factor (after elution as a single peak from a diethylaminoethyl cellulose column). Column RP 18 (300x7.8 mm dP = 10 pm) 40 °C eluent 0.04 M triethylamine acetate in water — acetonitrile (74 26), pH 5.6 flow rate 0.8 ml/min UV detection at 254 nm, 0.05 AUFS injection 100 pg in 100 pi. The a peak was due to the polypeptide of 53 amino acid residues (M =6040 g/mol), the p component did not contain the asparagine residue in position 1. (From Ref.77) with permission)...

Various types of adherent cells were grown on a coverslip, which was then laid on top of the LAPS chip. Measurements were made for acidification of (1) normal human epidermal keratinocytes stimulated by epidermal growth factor (EGP) or organic chemicals, and of (2) human uterine sarcoma cells as a response to doxorubicin and vincristine (chemotherapeutic drugs). In addition, the inhibition (by ribavirin) of the viral infection of murine fibroblastic L cells by vesicular stomatitis virus (VSV) was investigated by following the acidification rate. A limitation of these studies is the requirement for a low-buffered medium (low bicarbonate content) to achieve maximum sensitivity [847]. 

Fendly, B. M., Winget, M., Hudziak, R. M., Lipari, M. T., Napier, M. A., and Ullrich, A. (1990). Characterization of murine monoclonal antibodies reactive to either the human epidermal growth factor receptor or HER2/neu gene product. Cancer Res. 50,1550-1558. 

G. T. Montelione, K. Wuthrich, A. W. Burgess, E. C. Nice, G. Wagner, K. D. Gibson, and H. A. Scheraga, Biochemistry, 31, 236 (1992). Solution Structure of Murine Epidermal Growth Factor Determined by NMR Spectroscopy and Refined by Energy Minimization with Restraints. 

Todaro, G. J., De Larco, J. E., and Cohen, S. (1976). Transformation by murine and feline sarcoma viruses specifically blocks binding of epidermal growth factor to cells. Nature 264, 26-31. 

Peranovich, T.M.S. et al. Nitric oxide stimulates tyrosine phosphorylation in murine fihrohlasts in the absence and presence of epidermal growth factor, Biochem. J., 305, 613, 1995. 

The murine monoclonal antibody Ab4D5 directed against human epidermal growth factor receptor 2 pl85m R2 specifically inhibited proliferation of human... 

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