Ascites hepatoma

Kozuki, Y, Y Miura, and K Yagasaki. 2000. Inhibitory effects of carotenoids on the invasion of rat ascites hepatoma cells in culture. Cancer Lett 151 111-115. 

The formation of 0-seryl or 0-prolyl esters (Figure 1) of certain N-hydroxy arylamines has been inferred from the observations that highly reactive intermediates can be generated in vitro by incubation with ATP, serine or proline, and the corresponding aminoacyl tRNA synthetases (11,12,119). For example, activation of N-hydroxy-4-aminoquinoline-l-oxide (119,120), N-hydroxy-4-aminoazobenzene (11) and N-hydroxy-Trp-P-2 (121) to nucleic acid-bound products was demonstrated using seryl-tRNA synthetase from yeast or rat ascites hepatoma cells. More recently, hepatic cytosolic prolyl-, but not seryl-, tRNA synthetase was shown to activate N-hydroxy-Trp-P-2 (12) however, no activation was detectable for the N-hydroxy metabolites of AF, 3,2 -dimethyl-4-aminobiphenyl, or N -acetylbenzidine (122). 

Ward, W.S., Schmidt, W.N., Schmidt, C.A., and Hnilica, L.S. (1989) Cross-linking of Novikoff ascites hepatoma cytokeratin filaments. Biochemistry 24(16), 4429-4434. 

Various pyridazine-A-oxides (including cinnoline A-oxides) have been prepared as potential antitumour agents in Japan [276-278]. Among several 4-nitro-pyridazine 1-oxides tested for activity against rat ascites hepatoma AH-13, 3,6-dimethoxy-4-nitropyridazine 1-oxide (74) has been found to be the most potent compound a minimal effective dose of 5 mg/kg has been estimated [276], Also pyridazine A-oxides of type (75) bearing a bis(2-chloroethyl)ami-nomethyl side-chain at C-6 have been reported to be effective (0.5-5 mg/kg, i.p.) against AH-13 in rats [278]. Both types of compound (74), (75, R = H, Br), however, have been shown to be inactive against mouse lymphoid leukaemia L-1210. 

Tamiya-Koizumi, K., Umekawa, H., Yoshida, S., and Kojima, K., 1989, Existence of Mg " "-dependent, neutral sphingomyelinase in nuclei of rat ascites hepatoma cells. J. Biochem. 106 593-598,... 

CMP, <3> reaction with ATP, enzyme from Novikoff ascites hepatoma [3]) [3]... 

Orengo, A. Maness, P. Pyrimidine nucleoside monophosphate kinase from rat liver and rat Novikoff ascites hepatoma (EC 2.7.4.14). Methods Enzy-mol., 51, 321-331 (1978)... 

Rat ascites hepatoma cells DNA cross-linking + Wedrychowski et al. 1986... 

Rat ascites hepatoma CAH666> and rat esophageal (R1, R3> tumor cells... 

Induye, M., and Higashi, K., Properties of acetone insensitive phosphatase from ascites hepatoma of rat. J. Biochem. 54, 130-137 (1963). 

A thioredoxin has also been partially purified from L. leichtnannii (39). Although this thioredoxin is similar in size (MW approximately 12,000) to the E. coli thioredoxin, it is not able to function as a substrate for the E. coli thioredoxin reductase. More recently thioredoxins have been purified to homogeneity from rat Novikoff ascites hepatoma (127) and from calf liver (45). The properties of these two thioredoxins are quite similar (MW 11,400 and 12,000 respectively). The amino acid compositions however, are different. For instance the Novikoff tumor thioredoxin contains six half-cysteine residues, whereas calf liver thioredoxin has only four. Both these thioredoxins have a tendency to aggregate in the oxidized form. This aggregation is probably due to mixed sulfide formation between the additional sulfhydryl groups. Like yeast thioredoxin II, calf liver thioredoxin contains only one tryptophan residue unfortunately the tryptophan content of Novikoff tumor thioredoxin was not determined. 

Asterriquinone is oxidized with H2O2 in 0.1 A NaOH solution at room temperature to give Al-(r,r-dimethylallyl)indole-3-carboxylic acid. Asterriquinone shows a strong antitumor activity against Ehrlich ascites carcinoma and lymphatic leukemia LI210 in mice and against ascites hepatoma AH 13 in rat. The methylether of asterriquinone has remarkably decreased activity (186). 

---