N-Acetylmannosamine kinase

N-acetylmannosamine kinase N-acyl-D-mannosamine kinase UDP-GlcNAc-epimerase/ManNAc kinase <2-4> [8-13] acetylamidodeoxymannokinase acetylmannosamine kinase acylaminodeoxymannokinase acylmannosamine kinase acylmannosamine kinase (phosphorylating)... 

Zeitler, R. Giannis, A. Danneschewski, S. Henk, E. Henk, T. Bauer, C. Reutter, W. Sandhoff, K. Inhibition of N-acetylglucosamine kinase and N-acetylmannosamine kinase by 3-O-methyl-N-acetyl-D-glucosamine in vitro. Eur. J. Biochem., 204, 1165-1168 (1992)... 

Hinderlich, S. Sonnenschein, A. Reutter, W. Metal ion requirement of bi-functional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase from rat liver. BioMetals, 11, 253-258 (1998)... 

Lucka, L. Krause, M. Danker, K. Reutter, W. Horstkorte, R. Primary structure and expression analysis of human UDP-N-acetyl-glucosamine-2-epimerase/N-acetylmannosamine kinase, the bifimctional enzyme in neuraminic acid biosynthesis. FEBS Lett., 454, 341-344 (1999)... 

Another liver enzyme uses N-acetylglucosamine-6-phosphate as a substrate to yield N-acetylmannosa-mine-6-phosphate. An enzyme obtained from hog kidney converts free N-acetylglucosamine to N-acetyl-mannosamine. An N-acetylmannosamine kinase requiring magnesium and potassium for activity phos-phorylates N-acetylmannosamine to yield the N-ace-tylmannosamine-6-phosphate. ATP acts as phosphate donor. 

Reinke SO, Bidenschink C, Jay CM, Hinderlich S. (2009) Biochemical characterization of human and murine isoforms of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNB). Gly-coconj J 26, 415—422. 

Hinderlich S, Stasche R, ZeitlerR, Reutter W. (1997) A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic add biosynthesis of rat liver. Purification and charaderization of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase. J Biol Chem 272, 24313-24318. 

Hinderlich S, Salama B Eisenberg I et al. (2004) The homozygous M712T mutation of UDP-N-acetylgluco-samine 2-epimerase/N-acetylmannosamine kinase results in reduced enzyme activities but not in altered overall cellular sialylation in hereditary inclusion body myopathy. FEBS Lett 566, 105-109. 

Gagiannis D, Orthmann A, Danssmann I et al. (2007) Reduced sialylation status in UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE)-deficient mice. Glycoconj J 2A, 125-130. 

N-Acetylmannosamine-kinase decrease Morris hepatomas, Yoshida sarcoma, Yoshida ascites hepatoma Kikuchi et al. 1971, Grunholz 1978... 

CMP-N-acetylneuraminic acid (CMP-NeuAc). CMP-N-acetylneuraminic acid has been prepared enzymatically on small scales (> 0.5 mmol) from CTP and NeuAc, under catalysis by CMP-NeuAc synthetase (EC 2.7.7.43) [131l An improvement in this procedure, involving in situ production of CTP from CMP under adenylate kinase and pyruvate kinase catalysis, is suitable for multigram-scale synthesis11321. Adenylate kinase catalyzes the equilibration of CTP and CMP to CDP, which is subsequently phosphorylated by pyruvate kinase to provide CTP. A one-pot synthesis of CMP-NeuAc based on this procedure involves the in situ synthesis of NeuAc from N-acetylmannosamine and pyruvate, catalyzed by sialic acid aldolase (Fig. 11.3-12)[10S1. Chemical syntheses of CMP-NeuAc have also been reported11421. 

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