Mouse monocyte/macrophage cell line

Heat-treating (> 800 °C) fully deprived cristobal-ite of surface radicals and induced hydrophobicity, indicating that hydrophobicity is at least one of the surface properties determining the cytotoxic potential of a dust as tested on proUferating cells of the mouse monocyte macrophage cell line J774 (Fubini et al. 1999). 

The mouse monocyte-macrophage cell line RAW 264.7 (ATCC TIB-71) was cultured in DMEM containing 10% heat inactivated fetal bovine serum (GIBCO Life Technologies, Grand Island, NY). Except for transient transfection assay, cells were plated in dishes at a density of 4xlO /mL for 18-24 h before activation by LPS (1 pg/mL). Tested compounds were co-treated with LPS and the final DMSO (as vehicle) concentration was less than 0.2 % (v/v). 

The study of osteoclast differentiation is important for understanding potential new treatments for osteoporosis. Such therapies are typically explored in tissue culture models such as the Raw264 mouse monocytic cell line, which is capable of differentiation into functional multinuclear osteoclasts after treatment with the cytokine Rankl. Use of transformed cell lines raises the concern that results may not be extrapolated to normal tissue. To address this question, the transcriptional responses for Rankl treatment of the Raw264 cell line, and of two ex vivo primary cell systems (bone marrow macrophages, and hematopoetic stem cells) were compared using Affymetrix GeneChips [23]. The models proved to... 

Lactotransferrin receptors. The existence of a lactotransferrin receptor was first demonstrated by Van Snick and Masson in 1976 [191] at the surface of mouse peritoneal macrophages and lymphocytes. Since this discovery, the presence of lactotransferrin receptors has been demonstrated at the surface of various cells (for reviews, see refs. [156,158,192,193]) rabbit [48], mouse [165,166], monkey [167] and human [168] enterocytes human HT29 and Caco-2 enterocyte cell lines [194] human monocytes (reviewed in ref. [195]), human alveolar macrophages [196], human neutrophils [195,197], human resting lymphocytes [197], human activated lymphocytes [189], human Jurkat T cell line [190], human epithelial mammary cell line [198], human platelets [199,200] and megakaryocytes [201], hepatocytes [202,203] and in bacteria (for a review see refs. [204,205]). 

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