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Microscale methods method

A variety of microscale separation methods, performed in capillary format, employ a pool of techniqnes based on the differential migration velocities of analytes under the action of an electric field, which is referred to as capillary electromigration techniques. These separation techniques may depend on electrophoresis, the transport of charged species through a medium by an applied electric field, or may rely on electrically driven mobile phases to provide a true chromatographic separation system. Therefore, the electric field may either cause the separation mechanism or just promote the flow of a solution throughout the capillary tube, in which the separation takes place, or both. [Pg.156]

Analyses of sample sizes of approximately 100 beads are convenient at the reaction optimization stage in solid-phase organic syntheses. As in singlebead analyses, reactions in progress can be followed continually using microscale analysis methods. Several readily available spectroscopic accessories that facilitate such analyses are described below. [Pg.222]

Two isolation procedures based on methods in category 2 are described in this experiment, a large-scale and a microscale method. Each procedure yields plasmid DNA that is sufficiently pure for size analysis by agarose electrophoresis and for digestion by restriction enzymes as described in Experiment 15. [Pg.420]

As an alternative to the boiling method, students may perform the microscale isolation method. This version is more convenient to set up and complete, especially if facilities are limited. The procedure works well with unamplified or chloroamphenicol-amplified cultures. Each milliliter of an unamplified, overnight culture will yield approximately 1-2 /xg of plasmid DNA. This isolation procedure requires IV2 to 2 hours of student lab time if a cell culture is prepared in advance. [Pg.423]

DNA extraction and purification were traditionally accomplished using organic extraction and ultracentrifugation-based procedures, which are both time-consuming and not easily transferable to the microscale. Newer methods employ solid-phase extraction (SPE) on silica surfaces, glass fibers, modified magnetic beads, and ion-exchange resins—techniques that save time and are also more amenable to chip applications. [Pg.455]

The most unpredictable process in X-ray structure determination is the crystallization of the candidate protein into a form suitable for X-ray diffraction. Each protein requires a unique set of conditions to form crystals. Typically 100 mg of highly purified protein is required to determine the conditions that result in usable crystals of 0.1 to 0.3 mm size, although a size of 0.3 to 0.8 mm is preferred. The occurrence of crystals and the rate of crystallization are influenced by many factors such as protein purity, the solvent, concentration of added precipitants, pH, temperature, and the presence of ions and cofactors. The protein solution at a concentration of typically 5 to 20 mg/ml is allowed to slowly reach supersaturation by the removal of or by changing the composition of the solvent by liquid-liquid diffusion or vapor diffusion methods. Microscale methods have been developed to explore several crystallization conditions simultaneously using minimum amounts of the purified protein sample. Recently, use of the zero gravity atmosphere in space has been explored as a means of facilitating crystallization (Eisenberg and Hill, 1990 Branden and Tooze, 1991 Tomasselli et al, 1991). [Pg.172]

Neame, K. D., and Richards, T. G. (1972). Elementary kinetics of membrane transport. BlackweU. Nguyen, R. T., and Harvey, H. R. (1994). A rapid microscale method for the extraction and analysis of protein in marine samples. Mar. Chem. 45, 1-14. [Pg.376]

We have selected material for inclusion in Practical Skills in Chemistry based on our own teaching experience, highlighting those areas where our students have needed further guidance. As a result of our comprehensive cover of practical skills, some techniques such as microscale methods and specialized vacuum techniques have been omitted, but specific references are provided. Instead, we have attempted to provide sufficient detail so that students will have the skills to carry out experiments successfully and not produce poor data as a result of poor technique. [Pg.372]

Solid free-form [46, 47] Porous structure can be tailored to host tissue Protein and cell encapsulation possible Good interface with medical imaging Resolution needs to be improved to the microscale Some methods use organic solvents... [Pg.70]

The chemistry required to convert the oxide to other binary compounds is independent of the scale of operation. However, with microscale synthetic methods applied to radioactive materials, successful preparations are achieved more readily by carrying out the chemistry in situ, that is, in such a manner that eliminates, or at least minimizes, the necessity of having to "handle" the sample during or following its synthesis. Thus, actinide compounds are usually prepared in silica capillary tubes which can be flame sealed at the conclusion of a synthesis to provide the desired sample for study in a small volume, quartz container. A special feature of the preparation/vacuum system in the TRL is the capability to interrupt a synthesis, isolate (by means of a stopcock) and remove the sample, examine it in... [Pg.220]

Single drop extraction (SDE) or liquid-phase microextraction (LPME) is a recently developed microscale extraction method. In this method a single liquid drop is used as a collection phase. Small volumes of organic solvent (from 0.5 pi to 2.5 pi) are used. The collection phase must have a sufficiently high surface tension to form a drop which can be exposed to the analyte solution." When the extraction is finished, the single drop is injected into the GC. A scheme of such a device is shown in Eigure 2.8. [Pg.45]

Until recently, the work in this area has been insuflScient to warrant its review, but some papers on the biochemistry and pathophysiology of connective tissue (F9) are relevant. In consideration of the reports available, it must be borne in mind that comparisons made outside a particular circle of experiments may be invalid on account of the different conditions of the subjects, and conditions and methods for isolation, separation, and measurement of the macromolecules. Many such methods both for tissues and fluids have been reported (see reviews cited in Section 1.1), and it is imperative to ensure that the isolation and separation processes are effective (see B15, K12). Microscale methods have been devised to function on a few micrograms of material for component analysis (e.g., B16 see K17), but more particularly for the eomplete identification of a glycosaminoglycan on a basis of chemical structure (e.g., B13, B14). [Pg.41]

The principle of exciton coupling between vicinal benzoate chromophores has been extended to other aromatic carboxylic acid derivatives , including those of ring-substituted benzoic acids, 9-anthranoic acid (141) and p-methoxycinnamic acid (142). These derivatives have been widely used for the determination of the absolute stereochemistry in polyol natural products. The circular dichroism of the A -p-bromobenzoyl group combined with various 0-, 0,0 -di- and 0,0, 0"-tii-(p-bromobenzoyl) derivatives of 2-amino-2-deoxygalactopyranoside and an iV-anthranoyl group combined with tri-, tetra- and penta-p-methoxycinnamoyl derivatives of acyclic 1-amino polyols were studied to improve and develop microscale CD methods for the structural study of amino sugars. [Pg.143]

One microscale method developed for differential extraction involves a filter-based system and lysis using acoustic energy. The sample is first infused over a filter (size and material not indicated) in which the sperm cells ( 4-6 [tm diameter) pass through unimpeded and the much larger epithelial cells ( 50 [im diameter) are retained. The DNA is then extracted using ultrasonic disruption of the cells. Although it is too early to gauge the success of this method, filtration has been explored on the macroscale for this application without widespread success. [Pg.1066]

The LB [31] is another microscale method that is suited for the efficient treatment of polymer solution dynamics. It has recently been used to investigate the phase separation of binary flttids in the presence of solid particles. The LB method is originated... [Pg.159]

Microfluidics is promising for developing tools with integrated chenucal processing of proteomics analytes, by virtue of fast reaction kinetics. Sample purification and concentration can be carried out on the microscale by methods such as solid-phase extraction on hydrophobic... [Pg.2889]

Zhang, R. R., Takebe, T., Miyazaki, L., Takayama, M., Koike, H., Kimura, M., Enomura, M., Zheng, Y. W., Sekine, K., Taniguchi, H., 2014. Efficient hepatic differentiation of human induced pluripotent stem cells in a three-dimensional microscale culture. Methods Mol. Biol. 1210, 131-41. doi 10.1007/978-l-4939-1435-7 10. [Pg.345]

Ribatski et al. [2] presented a comparison between the microscale methods proposed by Kandlikar and Balasubramanian [80] and Zhang et al. [82] and the three-zone method by Thome et al. [85] against a broad database from the literature... [Pg.85]

In general, microscale methods, e.g. formation of mineral precipitates in the pore space of a waste body, will be employed rather than using large-scale enclosure systems such as clay covers or wall constructions (Wiles et al. 1988). An overview on various fields of environmental research and management to which mineralogical methods can be successfully applied has been given by Bambauer (1991). [Pg.174]

Liquid reagents and solutions are added to a reaction by several means, some of which are shown in Figure 7.9. For microscale experiments, the simplest approach is simply to add the liquid to the reaction by means of a Pasteur pipette. This method is shown in Figure 7.9A. In this technique, the system is open to the atmosphere. A second microscale method, shown in Figure 7.9B, is suitable for experiments in... [Pg.635]

In many experiments, it is necessary to remove excess solvent from a solution. An obvious approach is to allow the container to stand unstoppered in the hood for several hours until the solvent has evaporated. This method is generally not practical, however, and a quicker, more efficient means of evaporating solvents must be used. Figures 7.17 and 7.18 show several methods of removing solvents by evaporation. Figure 7.17 depicts microscale methods Figure 7.18 is devoted to large-scale procedures. [Pg.643]


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