Microfluidics hybridizations

Liu, Y. and Rauch, C.B., DNA probe attachment on plastic surfaces and microfluidic hybridization array channel devices with sample oscillation. Anal. Biochem., 317, 76-84, 2003. 

Li, L., Mustafi, D., Fu, Q., Tereshko, V., Chen, D. L., Tice, J. D., and Ismagilov, R. F. 2006. Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins. Proc. Natl. Acad. Sci. USA 103 19243M8. 

Lee H, Liu Y, Westervelt RM, Ham D (2006) IC/Microfluidic hybrid system for magnetic manipulation of biological cells. IEEE 41 1471-1480... 

K. Yamashita, Y. Yamaguchi, M. Miyazaki, H. Nakamura, H. Shimizu, H. Maeda, Direct observation of long-strand DNA conformational changing in microchannel flow and microfluidic hybridization assay. Anal. Biochem. 2004, 332, 274—279. 

The realization of complete bench-scale micro reactor set-ups is certainly still in its infancy. Nevertheless, the first investigations and proposals point at different generic concepts. First, this stems from the choice of the constructing elements for such set-ups. Either microfluidic components can be exclusively employed (the so-caUed monolithic concept) or mixed with conventional components (the so-called hybrid or multi-scale concept). Secondly, differences concerning the task of a micro-reactor plant exist. The design can be tailor-made for a specific reaction or process (specialty plant) or be designated for various processing tasks (multi-purpose plant). 

The hybrid integration of the IO chip with a microfluidic system is treated in detail in Sect. 10.5. 

Immunoaffinity chromatography (IAC), 6 400—402 12 137, 145 Immunoanalyzers, automated, 14 150 Immunoassay(s), 14 135-159. See also Immunoassay- DNA probe hybrid assays Immunoassay methods Immuno(bio)sensors antibody-antigen reaction, 14 136-138 basic technology in, 14 138-140 chemiluminescent, 14 150-151 classification of, 14 140-153 design of, 14 139-140 enzyme, 14 143-148 fluorescence, 14 148-150 highly specific, 14 153 historical perspective on, 14 136 microarrays and, 14 156—157 microfluidics in, 26 968—969 monoclonal versus polyclonal antibodies in, 14 152-153... 

Soe MJ, Okkels F, Saboimn D et al (2011) HistoFlex—a microfluidic device providing uniform flow conditions enabling highly sensitive, reproducible and quantitative in situ hybridizations. Lab Chip 11 3896-3907... 

A microfluidic device has been developed, which allows hybridization in 15 min and was able to discriminate between four Staphylococcus strains (134). A similar device has also been developed for cell lysis (135). The goal of these devices is to allow a clinical lab to take a patient specimen dispense it into a cassette and have DNA extraction, labeling, hybridization, and scanning occur automatically (136). 

DNA amplification (mostly by polymerase chain reaction, PCR) and other subsequent DNA analysis (including hybridization, sequencing, and genotyping) have been facilitated by the use of the microfluidic chip. These applications are then described in detail in subsequent sections. 

Krulevitch, P., Benett, W., Hamilton, J., Maghribi, M., Rose, K., Polymer-based packaging platform for hybrid microfluidic systems. Biomed. Microdevices 2002, 4(4), 301-308. 

Kuo, T.C., Cannon, D.M., Jr., Shannon, M.A., Bohn, P.W., Sweedler, J.V., Hybrid three-dimensional nanofluidic/microfluidic devices using molecular gates, Sensors Actuators A 2003, 102, 223-233. 

Seong, G.H., Zhan, W., Crooks, R.M., Fabrication of microchambers defined by photopolymerized hydrogels and weirs within microfluidic systems Application to DNA hybridization. Anal. Chem. 2002, 74, 3372-3377. 

Noerholm, M., Bruus, H., Jakobsen, M.H., Telleman, P., Ramsing, N.B., Polymer microfluidic chip for online monitoring of microarray hybridizations. Labchip 2004, 4, 28-37. 

---