Microbial enrichment

Hirschler A, J-F Rontani, D Raphel, R Matheron, J-C Bertrand (1998) Anaerobic degradation of hexadecan-2-one by a microbial enrichment culture under sulfate-reducing conditions. Appl Environ Microbiol 64 1576-1579. 

Many other methods for separating isotopes have been described. A partial list includes membrane and membrane pervaporation, thermal diffusion of liquids, mass diffusion, electrolysis and electro-migration, differential precipitation, solvent extraction, biological microbial enrichment, and more. Although not discussed in... 

Two important areas of research are underway to improve further the hybrid UV-03/soil disposal system. A UV monitor coupled with a computer is under investigation to make the unit self contained with regard to chemical analyses and to automate certain functions of the photochemical phase. Second, a microbial enrichment investigation utilizing UV-O3 products as substrates is in progress to select and possibly engineer degradators to accelerate the microbial phase. 

Postma J., Montanari M., Van den Boogert P.H J.F. Microbial enrichment to enhance the disease suppressive activity of compost. European J Soil Biol 2003 39 157-163. 

In a recent paper Steinbuchel studied the biological attack of microorganisms on rubber materials to evaluate the possible contributions of biotechnology for the development and recycling of used rubber products. Adaptation of microbial enrichment cultures with tire crumb material for several months resulted in enhanced growth of microorganisms, especially for NR and SBR. 

Kuipers B, Cullen WR, Mohn WM. 1999. Reductive dechlorination of nonachlorobiphenyls and selected octachlorobiphenyls by microbial enrichment cultures. Environ Sci Technol 33 3579-3585. 

The existing data for the reduction of N-nitrosoamines in anaerobic sediments and soils suggests that the aromatic substrates will be much more reactive. For example, N-nitrosodiethylamine and N-nitrosodisopropylamine were found to be very persistent in sediment slurries. In contrast, the reduction of N-nitroso-diphenylamine was quite fast (t,/2 = 1-5 days), resulting in the formation of diphenylamine (Weber, 1988). Formation of the intermediate, 1,1-diphenylhydrazine, was not observed. Several N-nitrosodialkylamines also have been found to be very persistent in flooded soils and microbial enrichments from bog sediments (Tate and Alexander, 1975). 

Brzostowicz PC, Walters DM, Thomas SM, Nagarajan V, Rouvieie PE. (2003). mRNA differential display in a microbial enrichment culture simultaneous identification of three cyclohexanonemonooxygenases from three species. Appl Envimn Micmbiol, 69, 334-342. 

The microbial community of the rotifers can also be shifted through a microbial enrichment (Makridis et al, 2000). This enrichment takes place after the rotifers have been harvested and prior to feeding them to the predator larvae. Makridis et al (2000) harvested and rinsed rotifers and exposed them to 5 x 10 CFU/mL of four probiotic species for 20-60 min. The probiotics remained associated with the rotifers for 4-24 h when placed in tanks with Tetraselmis sp. mimicking the conditions in turbot larvae tanks. 

A-acyl homoserine lactone-degrading microbial enrichment cultures isolated from Penaeus vannamei shrimp gut and their probiotic properties in Brachionus plicatilis cultures, FEMS Microbiol Ecol, 62, 45-53. 

The discovery and exploitation of enzymes in aldoxime-nitrile pathway nitrile hydratase, amidase, nitrilase, aldoxime dehydratase, etc., are shown along with the use of methodologies, such as organic chemistry, microbial screening by enrichment and acclimation culture techniques, enzyme purification, gene cloning, molecular screening by polymerase chain reaction (PCR). 

SCREENING FOR NEW MICROBIAL ENZYMES BY ENRICHMENT AND ACCLIMATION CULTURE TECHNIQUES... 

Traps with Bio-Sep beads amended with [ Cg]-benzene and [ C]-toluene were used to assess biodegradation in an aquifer (Geyer et al. 2005). Beads were lyophilized after exposure, lipids were extracted with chloroform-methanol, and the fatty acids and values analyzed. High enrichment of was observed in several fatty acids, which showed that the label from the substrates had been incorporated. In addition, there were differences in the abundance of the fatty acids in beads amended with benzene or toluene that suggested the existence of different microbial degradative populations. 

Middeldorp PJM, J de Wolf, AJB Zehnder, G Schraa (1997) Enrichment and properties of a 1,2,4-trichloro-benzene-dechlorinating microbial consortium. Appl Environ Microbiol 63 1225-1229. 

L. K. Jang, T. F. Yen, G. V. Chihngarian, and E. C. Donaldson. Bacterial migration through nutrient-enriched sandpack columns for in-situ recovery of oil. In E. C. Donaldson, G. V. Chihngarian, and T. F. Yen, editors. Microbial enhanced oil recovery, volume 22 of Developments in petroleum science, pages 151-164. Elsevier Science Ltd, 1989. 

U. Schenk, R. Manderschied, J. Hugen, and H.-J. Weigel, Effects of CO, enrichment and intraspecific competition on biomass partitioning, nitrogen content and microbial biomass carbon in soil of perennial ryegrass and white clover, J. Exp. Bot. 46 987 (1995). 

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