Metal hamster cells

DiPaolo JA, Casto BC. 1979. Quantitative studies of in vitro morphological transformation of Syrian hamster cells by inorganic metal salts. Cancer Res 39 3008-1013. 

Klein CB, Kargacin B, Su L. 1994. Metal mutagenic Chinese hamster cell lines. Environ Health Perspect 102(Suppl 3) 63-7. 

Larramendy ML, Popescu NC, DiPaolo JA. 1981. Induction by inorganic metal salts of sister chromatid exchanges and chromosome aberrations in human and Syrian hamster cell strands. Environ Mutagen 3 597-606. 

Transformation of Syrian Hamster Cells by Metals. When cultures of cells were exposed to two nickel compounds with different carcinogenic potencies, there were differences in the incidence of transformed colonies that formed (Table II). Treatment with amorphous NiS resulted in a low incidence of transformation while treatment with crystalline NiaS2 induced the appearance of numerous transformed colonies (Table II). In this series of experiments, control cultures had no transformed colonies however, in some experiments there was an occasional incidence of spontaneous transformation. The induction of transformation by... 

Carcinogenic metal compoimds such as crystalline Ni3S2 induced a dose-dependent statistically significant incidence of transformation in secondary cultures of Syrian hamster cells. A chemically related compound, amorphous NiS, which is not carcinogenic, resulted in no statistically significant incidences of transformed colonies. Untreated control cultures also had no statistically significant incidence of transformation. We have... 

Nocentini S (1987) Inhibition of DNA replication and repair by cadmium in mammalian cells. Protective interaction by zinc. Nucleic Acids Res 15 4211-4225 Oberly TJ, Piper CE, McDonald DS (1982) Mutagenicity of metal salts in the L5178Y mouse lymphoma assay. J Toxicol Environ Health 9 367-376 Ochi T, Ohsawa M (1983) Induction of 6-thioguanine-resistant mutants and singlestrand scission of DNA by cadmium chloride in cultured Chinese hamster cells. Mutat Res 111 69-78... 

Rossman TG, Stone D, Molina M, Troll W (1980) Absence of arsenite mutagenicity in E. coli and Chinese hamster cells. Environ Mutagen 2 371-379 Rossman TG, Molina M, Meyer LW (1984) The genetic toxicology of metal compounds. I. Induction of A prophage in E. coli WP2s (A). Environ Mutagen... 

Chromium has proved effective in counteracting the deleterious effects of cadmium in rats and of vanadium in chickens. High mortality rates and testicular atrophy occurred in rats subjected to an intraperitoneal injection of cadmium salts however, pretreatment with chromium ameliorated these effects (Stacey et al. 1983). The Cr-Cd relationship is not simple. In some cases, cadmium is known to suppress adverse effects induced in Chinese hamster (Cricetus spp.) ovary cells by Cr (Shimada et al. 1998). In southwestern Sweden, there was an 80% decline in chromium burdens in liver of the moose (Alces alces) between 1982 and 1992 from 0.21 to 0.07 mg Cr/kg FW (Frank et al. 1994). During this same period in this locale, moose experienced an unknown disease caused by a secondary copper deficiency due to elevated molybdenum levels as well as chromium deficiency and trace element imbalance (Frank et al. 1994). In chickens (Gallus sp.), 10 mg/kg of dietary chromium counteracted adverse effects on albumin metabolism and egg shell quality induced by 10 mg/kg of vanadium salts (Jensen and Maurice 1980). Additional research on the beneficial aspects of chromium in living resources appears warranted, especially where the organism is subjected to complex mixtures containing chromium and other potentially toxic heavy metals. 

Hamilton-Koch W, Snyder RD, Lavelle JM. 1986. Metal-induced DNA damage and repair in human diploid fibroblasts and Chinese hamster ovary cells. Chem Biol Interact 59 17-28. 

Miyaki M, Akamatsu N, Ono T, et al. 1979. Mutagenicity of metal cations in cultured cells from Chinese hamster. Mutat Res 68 259-263. 

Tan E-L, Williams MW, Schenley RL, et al. 1984. The toxicity of sixteen metallic compounds in Chinese hamster ovary cells. Toxicol Appl Pharmacol 74 330-336. 

Li, J.-H. and Rossman, T.G. (1991) Comutagenesis of sodium arsenite with ultraviolet radiation in Chinese hamster V79 cells. Biology of Metals, 4(4), 197-200. 

Mikalsen SO. 1990. Effects of heavy metal ions on intercellular communication in Syrian Hamster embryo cells. Carcinogenesis 11(9) 1621-1626. 

Figure 4, A tightly packed, piled-up normal Syrian hamster fetal cell colony. Treatment with some of the metal carcinogens induced a higher incidence of these tightly packed/piled up normal colonies. This type of colony was also present in untreated cultures.

Cultures of secondary Syrian hamster fetal cells were prepared with 10 cells per 35-mm plates. Following a 24-hr period of attachment the monolayer was treated two times for two days with various concentrations of the metal compounds as shown in the figure. The metal compounds were then removed by washing the mono-layer extensively with saline A and the cultures were incubated for 18-21 days with fresh Dulbecco s medium supplemented with 10% fetal bovine serum. The cultures were refed two times per week. The plates were then fixed and stained as described in Methods. Acetone was used to sterilize the metal compound. The number of transformed foci was determined per plate. Each point shown in the mean SEM for at least six plates. 

Syrian hamster fetal cells were treated with the various metal compounds shown in the table for 8-8 days. The compounds were removed by washing the cells with saline A. The cells were trypsinized and 10,000 ceUs were replated with fresh inedia into 35-mm plates and allowed to proliferate for two weeks. The plates containing... 

Mikalsen, S.-O., Rivedal, E., and Sanner, T. (1992). Heavy metal ions, cytotoxicity and gap junctional intercellular communication in Syrian hamster embryo cells. ATLA 20, 213-217. 

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