Syrian hamster cells fetal

Figure 1, Lightly packed (left) and densely packed (right) normal Syrian hamster fetal cells. Cultures of Syrian hamster fetal cells were obtained from minced whole embryos by trypsin treatment (see Methods for details). These cultures were untreated in parallel with cultures exposed to various carcinogens. The cultures were washed and the cells replated to form colonies in fresh medium. Following two weeks of incubation the cultures were fixed and stained with a crystal violet solution. Note the orderly growth of normal cells in the...

Figure 4, A tightly packed, piled-up normal Syrian hamster fetal cell colony. Treatment with some of the metal carcinogens induced a higher incidence of these tightly packed/piled up normal colonies. This type of colony was also present in untreated cultures.

Cultures of secondary Syrian hamster fetal cells were prepared with 10 cells per 35-mm plates. Following a 24-hr period of attachment the monolayer was treated two times for two days with various concentrations of the metal compounds as shown in the figure. The metal compounds were then removed by washing the mono-layer extensively with saline A and the cultures were incubated for 18-21 days with fresh Dulbecco s medium supplemented with 10% fetal bovine serum. The cultures were refed two times per week. The plates were then fixed and stained as described in Methods. Acetone was used to sterilize the metal compound. The number of transformed foci was determined per plate. Each point shown in the mean SEM for at least six plates. 

Syrian hamster fetal cells were treated with the various metal compounds shown in the table for 8-8 days. The compounds were removed by washing the cells with saline A. The cells were trypsinized and 10,000 ceUs were replated with fresh inedia into 35-mm plates and allowed to proliferate for two weeks. The plates containing... 

EFFECT OF CRYATALLINE NI3S2 AND AMORPHOUS NiS ON THE TRANSFORMATION OF SYRIAN HAMSTER FETAL CELLS IN TISSUE CULTURE... 

Third passage log phase cultures of Syrian hamster fetal cells were treated as described in the table. Cultures treated with benzopyrene and Ni3S2 were exposed to benzopyrene for 24 h prior to treatment with the metal. Cultures were treated with the metal compounds three times using a two-day exposure for each treatment. Cells were then removed from the plate by trypsini-zation and the number of cells present in each plate was determined with a hemocytometer. Five thousand or ten thousand cells were replated to form colonies into 100 mm diameter plates and the number of surviving colonies in each plate was counted. Each number shown in the table is the mean of four tissue culture plates. 

Costa, M- Nye, J. and Sunderman, F.W., Jr. "Morphological Transformation of Syrian Hamster Fetal Cells Induced... 

Syrian hamster embryo and mouse CSH lOTl/2 cells were used for transformation assays. Hamster cells were prepared and grown in Dulbecco s modified Eagle s medium supplemented with 10% fetal calf serum, penicillin (50 U ml" ), and streptomycin (50 jug ml" GIBCO), as previously described [4]. 

Emura M, Richter-Reichhelm HB, Schneider P. et al. 1980. Sensitivity of Syrian golden hamster fetal lung cells to benzo(a)pyrene and other polycyclic hydrocarbons in vitro. Toxicology 17 149-155. 

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