Cell factories

REASON FOR SUBMISSION This section should contain a brief explanation of the submission, e.g., "manufacturing change from roller bottle to cell factory" or "response to Information Request Letter of 1/9/97" or "Pediatric exclusivity determination request" or "to satisfy a subpart H postmarketing commitment". 

Steinborn, G., Boer, E., Scholz, A. et al. (2006) Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts. Microbial Cell Factories, 5, 33. 

Sorensen, H.P and Mortensen, K.K. (2005) Soluble expression of recombinant proteins in the cytoplasm of Pichia pastoris. Microbial Cell Factories, 4 (1), 1. 

Klein-Marcuschamera, D., Ajikumara, P.K. and Stephanopoulos, G. (2007) Engineering microbial cell factories for biosynthesis of isoprenoid molecules beyond lycopene. Trends in Biotechnology, 25, 417-424. 

Synthesis of the producer (cell factory) by growth and multiplication... 

The economic viability of the whole process is consequently determined, in addition to various market forces, by the efficiencies of these three stages and the velocities of the syntheses. Since the synthesis of poly(3HB) requires the cells both as a catalyst and as a reactor (cell factory), the overall yield is also influenced by the cellular content of the polymer. 

Punt, P.J., van Biezen, N., Conesa, A., Albers, A., Mangnus, J., and van den Hondel, C. 2002. Filamentous fungi as cell factories for heterologous protein production. Trends in Biotechnology 20(5), 200-206. 

Dinnis, D. and James, D. 2005. Engineering mammalian cell factories for improved recombinant monoclonal antibody production lessons from nature Biotechnology and Bioengineering 91(2), 180-189. 

Apart from new catalytic methods, cascade conversions require new process technologies, such as in situ product recovery, reactor design, and compartmental-ization. In the long term, part of the present-day stoichiometric chemistry as well as bio- and chemocatalytic conversions in multi-step syntheses will gradually be replaced by cascade catalysis in concert, and full fermentations by cell factory design, or combinations thereof (Fig. 13.17). 

E. R C. Rocha, P. Guerdoux-Jamet, I. Moszer, A. Viari, and A. Danchin, Implication of gene distribution in the bacterial chromosome for the bacterial cell factory. J. Biotech. 78, 209-219 (2000). 

Oksman-Caldentey KM, Inze D, Plant cell factories in the post-genomic eta New ways to produce designer secondary metaboHtes, Trends Plant Sci 9 433— 440, 2004. 

Based on these studies on biosynthetic pathways and metabolic engineering, it can be envisaged that once the plant cell factory has been assembled, the important determinants controlling the fluxes through the pathways are the... 

Fig. 2.3. FPLC purification of rAAV. Ten Nunc cell factories of rAAV-UFll...

Stirred-tank bioreactors Air-lift bioreactors Wave bioreactors Microcarrier-based systems Packed-bed bioreactors Fluidized-bed bioreactors Hollow-fiber bioreactors Bioreactors providing surfaces for attached cell growth (roller bottles, CellCube , Cell Factory)... 

On the other hand, the principle of CellCube and Cell Factory is to provide a large area for cell adhesion and proliferation in relatively compact devices. The latter consists of multiple trays, providing surface areas in the range of 700-25 000 cm2, for medium volumes of 0.2-8 L (Nunc, 2006). The system called CellCube provides a surface area in the range of 8500-85 000 cm2 and is fitted with an automated control system (Corning, 2006). 

Fig. 3.4. A Nunc cell factory. (Courtesy of Nunc and Gibco.)...

In order to undertake a large scale application of the obtained results, the zeolite MP was added to the electrolyte of Leclanche-type batteries, manufactured in the Yara Dry Cell Factory in Havana, Cuba [181]. The obtained batteries with the zeolite MP included in the electrolyte were tested under intermittent and continuous discharge procedures following the standard modus operandi of the Yara factory [181]. The results indicated that the batteries produced with the zeolite MP included in the electrolyte exhibited a better performance in comparison with the batteries produced following the standard technology [181]. 

Kleerebezemab, M., Hols, P., and Hugenholtz, J. 2000. Lactic acid bacteria as a cell factory rerouting of carbon metabolism in Lactococcus lactis by metabolic engineering. Enz. Microbial Technol., 26, 840-848. 

Figure 30.12 represents a cell factory model to produce several categories of products via fermentation and biocatalysis processes. Industrial-scale manufacturing aspects such as uses, synthesis methods, and costs of some of these products are reviewed below as well as in Chapter 32. 

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