Membrane transport energy requirements

Active transport (specific membrane carrier energy required operates against concentration gradient inhibitable saturable possible competition zero order)... 

Energy for maintenance is the energy required for survival, or non-growth related purposes. It includes activities such as active transport across membranes and turnover (replacement synthesis) of macromolecules. 

Figure 1. Solute transfer across an idealised eukaryote epithelium. The solute must move from the bulk solution (e.g. the external environment, or a body fluid) into an unstirred layer comprising water/mucus secretions, prior to binding to membrane-spanning carrier proteins (and the glycocalyx) which enable solute import. Solutes may then move across the cell by diffusion, or via specific cytosolic carriers, prior to export from the cell. Thus the overall process involves 1. Adsorption 2. Import 3. Solute transfer 4. Export. Some electrolytes may move between the cells (paracellular) by diffusion. The driving force for transport is often an energy-requiring pump (primary transport) located on the basolateral or serosal membrane (blood side), such as an ATPase. Outward electrochemical gradients for other solutes (X+) may drive import of the required solute (M+, metal ion) at the mucosal membrane by an antiporter (AP). Alternatively, the movement of X+ down its electrochemical gradient could enable M+ transport in the same direction across the membrane on a symporter (SP). A, diffusive anion such as chloride. Kl-6 refers to the equilibrium constants for each step in the metal transfer process, Kn indicates that there may be more than one intracellular compartment involved in storage. See the text for details...

These methods of solute transfer usually rely on a relatively low intracellular concentration of the solute of interest, so that it will readily diffuse into the cell down the electrochemical gradient (as in the case of ion channels). Alternatively, the solute may be moved into the cell using chemical energy derived from another solute moved in the same direction (co-transport) or opposite direction (countertransport) on the carrier protein (symporters and antiporters respectively). The transfer of the second solute is in turn dependent on an inward electrochemical gradient. Ultimately, these gradients are established by primary, energy-requiring solute pumps (e.g. ATPases), which, on most epithelia, are located on the basolateral/serosal membrane (see Section 5.2 for discussion of ATPases). 

Since active transport mechanisms require energy, the incubation temperature during the assay plays a crucial role. At 4°C, the fluidity of the cell membrane is reduced, the metabolism of the cell is downregulated, and energy-dependent transport processes are suppressed. Consequently, the amount of cell-associated target system refers mainly to the cytoadhesive fraction. In contrast, incubation at 37°C increases the fluidity of the cell membrane and the metabolic activity to an optimum, so both cytoadhesion and cytoinvasion occur at the same time. Thus, the uptake rate can be calculated from the difference in signal intensity measured upon incubation at both respective temperatures. 

Since the intracellular concentration of most amino acids is considerably greater than that in the plasma (Table 8.2), the transport of these amino acids is an energy-requiring process. This is achieved via the Na+ gradient across the plasma membrane, which is maintained by the ATP-dependent Na pump the NaVK+ ATPase (Figure 8.5). This is similar to that of the transport of glucose across the luminal membranes of epithelial cells in the gut and in the tubules is the kidney cortex. 

In addition to the processes described above, there still remains one further process which, at least in some cells or tissues, is required prior to the utilisation of ATP in the cytosol that is, the transport of energy within the cytosol, via a shuttle. The transport of ATP out and ADP into the mitochondrion, via the translocase, results in a high ATP/ ADP concentration ratio in the cytosol. However, a high ratio means that the actual concentration of ADP in the cytosol is low, which could result in slow diffusion of ADP from a site of ATP utilisation back to the inner mitochondrial membrane. If sufficiently slow, it could limit the rate of ATP generation. To overcome this, a process exists that transports energy within the cytosol, not by diffusion of ATP and ADP, but by the diffusion of phosphocreatine and creatine, a process known as the phosphocreatine/creatine shuttle. The reactions involved in the shuttle in muscle help to explain the significance of the process. They are ... 

Carrier-mediated passage of a molecular entity across a membrane (or other barrier). Facilitated transport follows saturation kinetics ie, the rate of transport at elevated concentrations of the transportable substrate reaches a maximum that reflects the concentration of carriers/transporters. In this respect, the kinetics resemble the Michaelis-Menten behavior of enzyme-catalyzed reactions. Facilitated diffusion systems are often stereo-specific, and they are subject to competitive inhibition. Facilitated transport systems are also distinguished from active transport systems which work against a concentration barrier and require a source of free energy. Simple diffusion often occurs in parallel to facilitated diffusion, and one must correct facilitated transport for the basal rate. This is usually evident when a plot of transport rate versus substrate concentration reaches a limiting nonzero rate at saturating substrate While the term passive transport has been used synonymously with facilitated transport, others have suggested that this term may be confused with or mistaken for simple diffusion. See Membrane Transport Kinetics... 

Naringenin, other flavones, and the cinnamic acids could behave like quercetin. Hence, at least some of the phenolic allelochemlcals could prevent the utilization of ATP energy required for transport of materials across cellular membranes by inhibiting the hydrolysis of ATP. Conceivably, alterations induced to the permeability of organelle membranes coupled... 

Figure 12.4 Mechanism of action of Na+/K+symport inhibitors (thiazides) on the distal convoluted tubule. As in the other parts of the nephron, Na+movement is powered by the energy-requiring sodium pump (P) in the basolateral membrane which exchanges intracellular Na+for K-i-in the extracellular fluid (ECF). The transport of Na-rand Cl- into the cell from the filtrate against the prevailing electrochemical gradient is facilitated by the symporter (S). The Na-Hons are then transported by the pump mechanism described above and the Cl- ions diffuse passively Into the ECF through ion channels in the basolateral membrane. Thiazide diuretics inhibit the symporter by disabling the Cl- binding site with the loss of Na-rand Cl- in the urine.

This must obviously be the opposite of passive transport. Active transport does require energy, usually in the form of the consumption of ATP or GTP, because the molecules are moving against the concentration gradient from an area of lower concentration to an area of higher concentration. The most well known active transport system is the Sodium-Potassium-ATPase Pump (Na" "- K+ZATPase) which maintains an imbalance of sodium and potassium ions inside and outside the membrane, respectively. See Figure 3. 

Nicotine (log D = 0.45 at pH 7.4) very rapidly enters the CNS, but this is believed to involve facilitated transport (Spector and Goldberg, 1982). Facilitated or active mechanisms are often suspected when transport seems not to follow the pH partition concepts, and can be proved by showing an energy requirement to exist, or that the carrier can become saturated, or that transport is competitively inhibited by a substance of similar structure. Special mechanisms exist for the transport of essential nutrients into the body, e.g. through the gastro-epithelial membrane, or for the selective disposal of excess electrolytes and polar metabolites from the body, e.g. 

ATP is a crucial intermediate for cells to maintain normal activities without a minimum level, the cell will die as systems will fail. ATP is the energy currency of the cell and is required for the synthesis of many substances such as macromolecules for structural and functional purposes, which the cell needs, but also for processes such as cell division, maintenance of the correct ionic balance, muscular and electrical activity, ciliary movement, membrane transporters, and specific ion channels. 

A second membrane transport system essential to oxidative phosphorylation is the phosphate translocase, which promotes symport of one H2PO4 and one H+ into the matrix. This transport process, too, is favored by the transmembrane proton gradient (Fig. 19-26). Notice that the process requires movement of one proton from the P to the N side of the inner membrane, consuming some of the energy of electron transfer. A complex of the ATP synthase and both translocases, the ATP synthasome, can be isolated from... 

The pathways involved in cyclic photophosphorylation in chloroplasts are not yet established. Electrons probably flow from the Fe-S centers Fdx, Fda, or Fdb back to cytochrome b563 or to the PQ pool as is indicated by the dashed line in Fig. 23-18. Cyclic flow around PSII is also possible. The photophosphorylation of inorganic phosphate to pyrophosphate (PP ) occurs in the chromatophores (vesicles derived from fragments of infolded photosynthetic membranes) from Rho-dospirillum rubrum. The PP formed in this way may be used in a variety of energy-requiring reactions in these bacteria.399 An example is formation of NADH by reverse electron transport. 

Peter Mitchell (Nobel Prize, 1978) of Great Britain was the first to realize, and to propose in his chemi-osmotic theory, that the energy required for die ADP-ATP reaction could be derived by an accretion of protons in the thylakoid sac to the point at which the electrochemical gradient across the membrane could effect the proton transport required as die driving force for this reaction. See also Phosphorylation (Photosynthetic). 

Chapters 14 and 18 Correct values for ATP production resulting from catabolism. Other texts do not take into account the energy required for ATP, ADP, and P transport across the inner mitochondrial membrane. This makes a substantial difference in the calculated energy yields. 

An H+ electrochemical gradient (ApH+) provides the energy required for active transport of all classical neurotransmitters into synaptic vesicles. The Mg2+-dependent vacuolar-type H+-ATPase (V-ATPase) that produces this gradient resides on internal membranes of the secretory pathway, in particular endosomes and lysosomes (vacuole in yeast) as well as secretory vesicles (Figure 3). In terms of both structure and function, this pump resembles the F-type ATPases of bacteria, mitochondria and chloroplasts, and differs from the P-type ATPases expressed at the plasma membrane of mammalian cells (e.g., the Na+/K+-, gastric H+/K+-and muscle Ca2+-ATPases) (Forgac, 1989 Nelson, 1992). The vacuolar and F0F1... 

Active transport of a molecule across a membrane against its concentration gradient requires an input of metabolic energy. In the case of ATP-driven active transport, the energy required for the transport of the molecule (Na+, K+, Ca2+ or H+) across the membrane is derived from the coupled hydrolysis of ATP (e.g Na+/K+-ATPase). In ion-driven active transport, the movement of the molecule to be transported across the membrane is coupled to the movement of an ion (either Na+ or H+) down its concentration gradient. If both the molecule to be transported and the ion move in the same direction across the membrane, the process is called symport (e.g. Na+/glucose transporter) if the molecule and the ion move in opposite directions it is called antiport (e.g. erythrocyte band 3 anion transporter). 

Passive transport The passive transport of molecules across a membrane does not require an input of metabolic energy. The rate of transport (diffusion) is proportional to the concentration gradient of the molecule across the membrane. There are two types of passive transport simple diffusion and facilitated diffusion. 

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