Quantitation mass spectrometry

Tan [71] devised a rapid simple sample preparation technique for analysing polyaromatic hydrocarbons in sediments. Polyaromatic hydrocarbons are removed from the sediment by ultrasonic extraction and isolated by solvent partition and silica gel column chromatography. The sulphur removal step is combined into the ultrasonic extraction procedure. Identification of polyaromatic hydrocarbon is carried by gas chromatography alone and in conjunction with mass spectrometry. Quantitative determination is achieved by addition of known amounts of standard compounds using flame ionization and multiple ion detectors. 

In conventional mass spectrometry, quantitative determinations are often carried out by using selected-ion monitoring (SIM), i.e. by monitoring the intensities of a small number of ions characteristic of the analyte of interest (see Section 3.5.2.1 below). 

Frangopol and Morariu have edited a seminar on procaine and related drugs, methods of analysis, and effects on cell membranes [29]. Items covered include studies on Romanian drugs by mass spectrometry and gas chromatography-mass spectrometry, quantitative and qualitative determination of procaine in biological samples, separation and quantitative thin layer chromatography determination of procaine... 

Table 2.1.3 Multiple reaction monitoring of amino acids for their tandem mass spectrometry quantitation. In daily practise not all mentioned amino acids are measured in one run, but a set of ten dedicated evaluation programs has been developed, covering groups of amino acids associated with groups of disorders. Amino acids presented in italics indicate stable-isotope-labeled internal standards ... 

Draisci, R., Marchiafava, C., Palleschi, L., Cammarata, R, and Cavalli, S. (2001). Accelerated solvent extraction and liquid chromatography-tandem mass spectrometry quantitation of corticosteroid residues in bovine liver. J. Chromatogr. B Biomed Sci. Appl. 753 217-223. 

Doig MV (2000) Applications of mass spectrometry quantitative mass spectrometry. In Venn RF (ed) Principles and practice of bioanalysis. Taylor Francis, London... 

Taylor AW, Bruno RS, Frei B, Traber MG. Benefits of prolonged gradient separation for high-performance liquid 26. chromatography-tandem mass spectrometry quantitation of plasma... 

Reaction products were identified by Mass Spectrometry quantitative analysis was carried out by Gas Chromatography (GC). 

The same preparation was performed with wheat. At least eight portions of each of the corn and wheat samples were extracted, diluted, derivatized, and analyzed by mass spectrometry. Quantitation of the DON in the sample matrices was accomplished by measurement of the peak area of the molecular ion of the DON-HFB in the sample and comparing this to the peak area of the same ion as the standard. 

A similar problem associated with synthetic peptides of chain length > 5 amino acid residues is the identification of side reactions. Those involve substitutions on imidazole-, phenol- and indole moieties of histidine, tyrosine, and tryptophane as well as conversions, transamidation, and cyclizations of aspartic and glutamic side chains. As long as structural variations are stable under the reaction conditions of an Edman degradation, they can be detected from the proper phenylthiohydantoines in combination with H-NMR- and mass spectrometry. Quantitative amino acid analyses of impure peptides after acidic total hydrolysis do not indicate those structural deviations between main product and contaminations. 

By mass spectrometry Quantition can be performed by several methods, such as Colorimetry spectrophotometry Spectrofluorimetry HPLC... 

Industrial Analysis with Vibrational Spectroscopy Ionization Methods in Organic Mass Spectrometry Quantitative Millimetre Wavelength Spectrometry Glow Discharge Optical Emission Spectroscopy A Practical Guide Chemometrics in Analytical Spectroscopy, 2nd Edition Raman Spectroscopy in Archaeology and Art History Basic Chemometric Techniques in Atomic Spectroscopy Biomedical Applications of Synchrotron Infrared Microspectroscopy... 

---