Laser desorption/ionization mass spectrometry quantitation

Liquid chromatography/mass spectrometry Lower limit of detection Limit of detection Limit of quantitation Florseshoe crab hemocyanin Liquid scintillation counting Matrix-assisted laser desorption/ ionization mass spectrometry m -Maleimidobenzoy 1-A -Hydroxysuccinimide 1 -Cyclohexyl-3-(2-Morptiolino-ethyl)carbodiimide rnetlio-/ -Toluenesulfonate (same as CDI)... 

O. A. Mirgorodskaya, Y. P. Kozmin, M. I. Titov, R. Kbmer, C. P. Sonksen, and P. Roepstorff. Quantitation of Peptides and Proteins by Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry Using lsO-Labeled Internal Standards. Rapid Commun. Mass Spectrom., 14(2000) 1226-1232. 

Zabet-Moghaddam, M., Heinzle, E. and Tholey, A., Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry using ionic liquid matrices. Rapid Commun. Mass Spec., 18,141, 2004. 

Jespersen, S., Niessen, W. M. A., Tjaden, U. R., and van der Greef, J. (1995). Quantitative bioanalysis using matrix-assisted laser desorption/ionization mass spectrometry. J. Mass Spectrom. 30 357-364. 

Sleno, L., and Volmer, D. A. (2005). Some fundamental and technical aspects of the quantitative analysis of pharmaceutical drugs by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 19 1928-1936. 

Wang, Q. Jakubowski, J. A. Sweedier, J. V. Bohn, P. W., Quantitative submonolayer spatial mapping of Arg-Gly-Asp-containing peptide organo-mercaptan gradients on gold with matrix-assisted laser desorption/ionization mass spectrometry, Anal. Chem. 2004, 76, 1-8... 

Aptamers hold great promise as molecular recognition tools for their incorporation into analytical devices, and in particular, they can be used as immobilized ligands in separation technologies (Clark and Remcho, 2002), as affinity probes in capillary electrophoresis-based quantitative assays (Kotia and McGown, 2000), in affinity matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)(Dick and McGown, 2004), and as biocomponents in biosensors (Tombelli et al., 2005). Other aptamer-based bioanalytical applications have been reviewed (Yon et al., 2003 Mukhopadhyay, 2005). 

Nicola AJ, Gusev AI, Hercules DM. Direct quantitative analysis from thin-layer chromatography plates using matrix-assisted laser desorption/ionization mass spectrometry. Appl Spectrosc 1996 50 1479-1482. 

Sleno L, Volmer DA. Assessing the properties of internal standards for quantitative matrix-assisted laser desorption/ionization mass spectrometry of small molecules. Rapid Commun Mass Spectrom 2006 20 1517-1524. 

Figure 8 Scheme for differential labeling of two samples with do/da acrylamide (alkylation of Cys residues). The central map refers to rat sera, labeled separately with either do- or ds-acrylamide and mixed in a 30 70% ratio. (Reproduced from Gehanne S, Cecconi D, Carbon L, et al. (2002) Quantitative analysis of two-dimensional gel-separated proteins using isotopically marked alkylating agents and matrix-assisted laser desorption/ionization mass spectrometry. Reipid Communications in Mass Spectrometry 16 1692-1698.)... 

Vaidyanathan, S., Goodacre, R. (2007) Quantitative Detection of Metabolites Using Matrix-assisted Laser Desorption/Ionization Mass Spectrometry with 9-Aminoacridine as the Matrix. Rapid Commun. Mass Spectrom. 21 2072-2078. 

Seetharaman, V, Royston, G. (2007) Quantitative detection of metabolites using matrix-assisted laser desorption/ionization mass spectrometry with 9-aminoacridine as the matrix. Rapid Commun Mass Spectrom, 21, 2072-2078. 

May, L.A. et al.. Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry. Anal. Biochem., 337, 62, 2005. 

In a separate study, a protocol for Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) has been proposed.18 This IMS technique provides a new approach to visualize spatial distribution of thousands of molecular species, including peptides, proteins, and their metabolites in two- or three-dimensional levels. This approach may also provide a straightforward method of determining the tissue distribution of multiple peptides or proteins in a quantitative manner.18 Chu et al.19 reported a nondestructive molecular extraction method to obtain proteins from a single FFPE or frozen tissue section, without destroying the tissue morphology, such... 

This proposal describes the development of a new, systematic approach for qualitatively and quantitatively studying surface-biomolecule interactions by matrix-assisted laser desorption ionization (MALDl) mass spectrometry (MS). This methodology is being developed because of the profound importance that surface-biomolecule interactions play in applications where biomaterials come into contact with complex biological fluids, it can readily be shown that undesired reactions occurring in response to surface-biomolecule contact (protein adsorption, biofouling, immune response activation, etc.) lead to enormous economic and human costs. Thus, the development of analytical methodologies that allow for efficient assessment of the properties of new biomaterials and/or the study of detailed fundamental processes initiated upon surface-biomolecule contact are of critical value ... 

Muddiman, D. C., Gusev, A. I., and Hercules, D. M. (1996). Application of secondary ion and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the quantitative analysis of biological molecules. Mass Spectrom. Rev. 14 383-429. 

Kang, M. J., Tholey, A., and Heinzle, E. (2000). Quantitation of low molecular mass substrates and products of enzyme catalyzed reactions using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 14 1972-1978. 

---