Thin layer chromatography quantitation

Getz, M. E., in J. C. Touehstone, D. Rogers Thin-Layer Chromatography Quantitative Environmental and Clinical Application. J. Wiley Sons, New York-Chiehester-Bris-bane-Toronto 1980. 

Touchstone, J.C. et al., in Thin Layer Chromatography Quantitative Environmental Clinical Applications, Touchstone, J.C. and Rogers, D., Eds., John Wiley Sons, New York, 1980, pp. 151-157. 

C. K. Cross. In Thin Layer Chromatography Quantitative Environmental and Clinical Applications (J. C. Touchstone and D. Rogers, eds.). Wiley, New York, pp 300-308 (1979). 

Any material with a capillary structure that can be made to adhere as a layer can be used for thin-layer chromatography. Quantitative determinations by this technique are bound to increase as more and more laboratories obtain scanning equipment. This will give impetus to the companies who make commercially-prepared plates to produce them with more care and uniformity. 

Petrovic, M., M. Kastelan-Macan, K. Lazaric, and S. Babic, Vahdation of thin layer chromatography quantitation with CCD camera and slit-scanning densitometer, / AOACInt. 82 25-39 (1999). 

Sethi, ED. HPTLC, High Performance Thin-Layer Chromatography, Quantitative Analysis of Pharmaceutical Formulations, CBS Publisher Distributors, New Dehli (India) 1996, ISBN... 

To determine the phosphoHpid and fatty acid compositions chromatographic methods (28) like gas chromatography (gc), thin-layer chromatography (tic), and high performance Hquid chromatography (hlpc) are used. Newer methods for quantitative deterrnination of different phosphoHpid classes include P-nmr (29). 

Analysis. Dilute aqueous solutions of hydroxyhydroquiaone turn blue-green temporarily when mixed with ferric chloride. The solutions darken upon addition of small amounts, and turn red with additions of larger amounts of sodium carbonate. Derivatives used for identification are the picrate, which forms orange-red needles (mp of 96°C), and the triacetate (mp 96—97°C). Thin-layer chromatography and Hquid chromatography are well suited for the quahtative and quantitative estimation of hydroxyhydroquiaone (93,94). 

The new way of quantitative determination of the ascorbic acid (AC) by means of ion-pair thin layer chromatography (TLC) in organo-aqueous mobile phases containing cetyltrimethylammonium bromide (CTAB) has been alaborated. 

Commercially available pre-coated plates with a variety of adsorbents are generally very good for quantitative work because they are of a standard quality. Plates of a standardised silica gel 60 (as medium porosity silica gel with a mean porosity of 6mm) released by Merck have a specific surface of 500 m /g and a specific pore volume of 0.75 mL/g. They are so efficient that they have been called high performance thin layer chromatography (HPTLC) plates (Ropphahn and Halpap J Chromatogr 112 81 1975). In another variant of thin layer chromatography the... 

Bohrer, I. M. Evaluation Systems in Quantitative Thin-Layer Chromatography, 126,95-118 (1984). Boekelheide, V. Syntheses and Properties of the [20] Cyclophanes, 113, 87-143 (1983). 

Ebel, S. Evaluation and Calibration in Quantitative Thin-Layer Chromatography, 126, 71-94... 

The present chapter is largely concerned with HPLC, together with a summary of developments in quantitative thin-layer chromatography, but a brief account of the various types of liquid chromatography is given first together with a guide to the choice of appropriate separation mode. 

The purpose of the experiment is to illustrate the elution technique for the recovery of pure substances after their separation by thin-layer chromatography. The experiment can be readily extended to include the quantitative determination of the recovered substances. 

W E Court, Quantitative thin-layer chromatography using elution techniques. In Quantitative Paper and Thin-Layer Chromatography E J Shellard (Ed) Academic Press, New York, 1968, p 29... 

J C Touchstone (Ed), Quantitative Thin-Layer Chromatography, Wiley, New York, 1973... 

The multiplicity of responses makes thin-layer chromatography not particularly suited for pyrethrum analysis, either qualitative or quantitative. It did confirm, however, that the crude oleoresin contains several pyrethroid compounds in substantial quantity, as previously shown by gas chromatography work. 

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