Mass spectrometry, quantitative

Millard, B.J., Quantitative Mass Spectrometry, Heyden, London, 1978. 

Quantitative mass spectrometry, also used for pharmaceutical appHcations, involves the use of isotopicaHy labeled internal standards for method calibration and the calculation of percent recoveries (9). Maximum sensitivity is obtained when the mass spectrometer is set to monitor only a few ions, which are characteristic of the target compounds to be quantified, a procedure known as the selected ion monitoring mode (sim). When chlorinated species are to be detected, then two ions from the isotopic envelope can be monitored, and confirmation of the target compound can be based not only on the gc retention time and the mass, but on the ratio of the two ion abundances being close to the theoretically expected value. The spectrometer cycles through the ions in the shortest possible time. This avoids compromising the chromatographic resolution of the gc, because even after extraction the sample contains many compounds in addition to the analyte. To increase sensitivity, some methods use sample concentration techniques. 

A proteomic study of the HeLa cell proteins in raft fractions, identified by these criteria and estimated by quantitative mass spectrometry, has identified 241 authentic raft proteins [23]. This analysis found that the raft proteins ... 

Figures 4.31(c), 4.36 and 13.3 from Snyder and Kirkland, Introduction to Modern Liquid Chromatography, 2nd edn., (1979) 9.41(a), (b) and (c) from Cooper, Spectroscopic Techniques for Organic Chemists (1980) 9.46 from Millard, Quantitative Mass Spectrometry (1978) 4.17, 4.18, 4.31 (a), 4.33, 4.34(a), 4.37, 4.38, 4.43 and 4.45 from Smith, Gas and Liquid Chromatography in Analytical Chemistry (1988) figures 4.42 and 13.2 from Berridge, Techniques for the Automated Optimisation of Hplc Separations (1985) reproduced by permission of John Wiley and Sons Limited 11.1, 11.5, 11.6, 11.12, 11.13, 11.14, 11.18 and 11.19 from Wendlandt, Thermal Analysis, 3rd edn., (1986) reprinted by permission of John Wiley and Sons Inc., all rights reserved.

Isotope Dilution Mass Spectrometry (IDMS) A quantitative mass spectrometry technique in which an isotopically enriched compound is used as an internal standard. See Chapter 14 for a more detailed explanation. 

Quantitative Mass Spectrometry. Curr Opin Biotechnol, 19, 331. 

A. Yergey, J. P. Gale, and M. W. Ducan, Principles of Quantitative Mass Spectrometry, ASMS Short Course, 2001. 

Braida L, Crovella S, Boniotto M, et al (2001) A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and amino acids in amniotic fluid. Prenat Diagn 21 543-546... 

Foltz, R. L. 1978. Quantitative analysis of abused drugs in physiological fluids by gas chromatography/chemical ionization mass spectrometry. In Quantitative Mass Spectrometry in Life Sciences II, De Leenheer, A. P. Roncucci, R. R. Van Peteghem, C., eds., Amsterdam Elsevier, 39-62. 

Self, R. 1979. A polemic on the uses and functions of deuterated analogues in quantitative mass spectrometry. Biomed. Mass Spectrom., 6,315-316. 

Peripheral blood CAD 117 Quantitative mass spectrometry-based metabolic profiling Arginine Ornithine Alanine Proline Leucine/isoleucine Valine Glutamate/glutamine Phenylalanine Glycine (23)... 

Doig MV (2000) Applications of mass spectrometry quantitative mass spectrometry. In Venn RF (ed) Principles and practice of bioanalysis. Taylor Francis, London... 

In quantitative mass spectrometry, the signal intensity depends not only on the amount of sample, but also on a number of other variables such as the ionization yield, focusing of the ion beam, and the amplification factor of the detector. As it is very difficult to keep these parameters constant over the whole period of analysis, nearly all quantitative applications of MS are based on a comparison of the ion current obtained from the component of interest, with the ion current obtained from a standard. In quantitative SIM this can be accomplished either by the continuous admission of a reference sample at a constant rate, concurrently with the sample under investigation, or by the use of an internal standard (IS) which is added to the sample prior to MS analysis (Halpern, 1981). The choice of this IS is of primary importance in the design of a new assay and was subject to some controversy in the late 1970s (Claeys et al., 1977 Lee and Millard, 1975 Millard, 1978b Self, 1979). Ideally, an IS should compensate for all possible losses during sample isolation, purification, derivatization, and separation steps and at the same time minimize variances due to the measurement process. In practice, the... 

Adlercreutz, H. (1977). Quantitative mass spectrometry of endogenous and exogenous steroids in metabolic studies in man. In "Quantitative Mass Spectrometry in Life Sciences (A. P. De Leenheer and R. R. Roncucci, eds.), Vol. 1, pp. 15-28. Elsevier, Amsterdam. 

Gaskell, S. J. (1982). New developments in quantitative mass spectrometry. Trends Anal. Chem. 1, 110-113. 

Muskiet, F. A. (1982). The use of quantitative mass spectrometry for the determination of low molecular tumor markers. Int. Symp. Quant. Mass Spectrom. Life Sci. 4th, Ghent, May (Plenary Lecture). 

Quantitative Mass Spectrometry for Comparative and Funaional Proteomics 67... 

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