Mass spectrometry profiling

Black, G.E. and Fox, A., Liquid chromatography with electrospray ionization tandem mass spectrometry profiling carbohydrates in whole bacterial cell hydrolysates, in Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry, ACS Symposium Series, Snyder, A.P. and Anaheim, C.A., Fids., Washington, D.C., 1995, chap. 4. 

Smedsgaard, J. and Frisvad, J.C., Using direct electrospray mass spectrometry profiling of crude extracts, J. Microbiol. Methods, 25, 5, 1996. 

Figure 2 Mass spectrometry profiles of pulsing 1 ml 02 into the propylene/He flow.

Merkel, D., et al., Proteomic study of human bronchoalveolar lavage fluids from smokers with chronic obstructive pulmonary disease by combining surface-enhanced laser desorp-tion/ionization-mass spectrometry profiling with mass spectrometric protein identification, Proteomics. 5, 11, 2972, 2005. 

Fig. 7.1.3 High-performance liquid chromatography/electron spin ionization-tandem mass spectrometry profiles of urines from patients with a dihydropyrimidine dehydrogenase (upper panel), dihydropyrimidinase (middle panel) and a /Tureidopropionase deficiency (lower panel). The inserts show the profiles of a control urine, note the differences in scale. N-C-fi-ala-nine N-Carbamyl-j6-alanine, N-C-fi-AIB N-carbamyl-/Taminoisobutyric acid...

Fig. 15 Capillary electrophoresis-mass spectrometry profile of two peptides preconcentrated on-line prior to separation. (A) Electropherogram of neurotensin (1) and angiotensin (2), concentrated and purified by C-18 immobilized to porous beads and monitored at 195 nm after separation by CE. (B) Total ion-current electropherogram of the separated peptides. The experimental conditions were similar to those described in Ref. 120 for gonadotropin-releasing hormone. The limits of detection for the peptides were approximately 1 to 5 ng/mL, depending primarily on the quality of the analyte concentrator-microreactor.

Identification of different reaction products or different peaks from chromatograms is done by using standards or performing mass spectrometry. MALDI-TOF is an ionization technique frequently used. Also, combined setups can be found like LC-MS or UPLC-ESI-MS (electron spray ionization mass spectrometry) profiles. ... 

Want, E.J., Coen, M., Masson, P, Kenn, H.C., Pearce, IT, ReUy, M.D., Robertson, D.G., Rohde, C.M., Holmes, E., Lindon, J.C., Plumb, R.S., Nicholson, J.K. (2010) Ultra performance hqnid chromatography-mass spectrometry profiling of bile acid metabolites in bioflnids application to experimental toxicology stndies. Anal. Chem., 82(12), 5282-5289. 

Bald2ovd T, Sedo O, Stefanid P, et al. Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion. Rapid Commun Mass Spectrom. 2014 28 1855-61. doi 10.1002/rcm.6966. 

Sedo O, Vavrova A, Vad urova M, Tvizova L, Zdrahal Z. The inlluenee of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling. Rapid Commun Mass Sp. 2013 27(24) 2729-36. 

Taking into account Fe solubiiity data obtained from the secondary ion mass spectrometry profiles, evidence was provided that D was representative of... 

Vandervorst W, Shepherd FR (1987) Secondary ion mass spectrometry profiling of shallow, implanted layers using quadrupole and magnetic sector instruments. J Vac Sci Technol A... 

Jones, E.E., Powers, T.W, Neely, B.A., Cazares, L.H., Troyer, D.A., Parker, A.S. and Drake, R.R. (2014) MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma. Proteomics 14, 924-935. 

Eibl, G., Bernardo, K., Koal, T., Ramsay, S.L., Weinberger, K.M. and Graber, A. (2008) Isotope correction of mass spectrometry profiles. Rapid Commun. Mass Spectrom. 22, 2248-2252. 

Khakimov B, Amigo JM, Bak S, Engelsen SB. Plant metabolomics resolution and quantification of elusive peaks in liquid chromatography-mass spectrometry profiles of complex plant extracts using multi-way decomposition methods. J Chromatogr A 2012 1266 84-94. 

Secondary ion mass spectrometry (SIMS) is by far the most sensitive surface teclmique, but also the most difficult one to quantify. SIMS is very popular in materials research for making concentration depth profiles and chemical maps of the surface. For a more extensive treatment of SIMS the reader is referred to [3] and [14. 15 and 16]. The principle of SIMS is conceptually simple When a surface is exposed to a beam of ions... 

Wilson, R.G., Stevie, F.A., and Magee, C.W., Secondary Ion Mass Spectrometry A Practical Handbook for Depth Profiling and Bulk Impurity Analysis, Wiley, Chichester, U.K., 1989. 

Sputtered Neutral Mass Spectrometry (SNMS) is the mass spectrometric analysis of sputtered atoms ejected from a solid surface by energetic ion bombardment. The sputtered atoms are ionized for mass spectrometric analysis by a mechanism separate from the sputtering atomization. As such, SNMS is complementary to Secondary Ion Mass Spectrometry (SIMS), which is the mass spectrometric analysis of sputtered ions, as distinct from sputtered atoms. The forte of SNMS analysis, compared to SIMS, is the accurate measurement of concentration depth profiles through chemically complex thin-film structures, including interfaces, with excellent depth resolution and to trace concentration levels. Genetically both SALI and GDMS are specific examples of SNMS. In this article we concentrate on post ionization only by electron impact. 

This article discusses why one would choose nonresonant multiphoton ionization for mass spectrometry of solid surfaces. Examples are given for depth profiling by this method along with thermal desorption studies. 

Neutron activation also has been combined with accelerator mass spectrometry and has been demonstrated to have part-per-billion sensitivities fer bulk nitrogen analysis in silicon. This combination was also used to obtain depth profile of Cl in silicon semiconductors. ... 

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