Lucas method

Polar and quantum effects (in H2, D2, and He) can be taken into account by multiplying equation (2-51) by factors given by Lucas (1980). The data needed to apply the Lucas method are tabulated next (Reid et al., 1987). 

Leaney, P. G. 1996b Case Experience with Hitachi, Lucas and Boothroyd-Dewhurst DFA Methods. In Huang, G. Q. (ed.). Design for X - Concurrent Engineering Imperatives. London Chapman Hall. 

The beaker is chilled in an ice bath for 1 hour. The crystalline diacetate that separates is collected on a Buchner funnel and washed with three 20-ml. portions of cold water. After drying for 30 minutes on the funnel with suction, the diacetate is dried overnight in a vacuum desiccator containing calcium chloride (Note 4). The dried diacetate weighs 26.7-29.3 g. (83-91%) and melts at 158-159° with decomposition. The purity of the diacetate, determined by the titration method of Lucas, Kennedy, and Formo,3 is 97-98%, which is good enough for most purposes. The purity can be increased to 99-100% by a recrystallization from 5M acetic acid. 

S.J. Gee, A.D. Lucas, and B.D. Hammock, Using immunochemical methods to analyze for biomarkers of exposure, in Methods of Pesticide Exposure Assessment, ed. PB. Curry,... 

Dillon, Young, and Lucas (5) described a method for determining dibromobutanes in 99% methanol. Dillon ( ) expanded this work to include a procedure for determining ethylene dibromide. Brenner and Poland (1) described a procedure in which they determined 1.000 mg. of ethylene dibromide with a recovery of 65.8%. Equations 1 and 2 show the main reactions that occur. 

De Luca M., McElroy W. D., Purification and properties of firefly luciferase, Methods Enzymol. 1978 57 3-15. 

Buyens K, Lucas B, Raemdonck K, Braeckmans K, Vercammen J, Hendrix J, Engelborghs Y, De Smedt SC, Sanders NN (2008) A fast and sensitive method for measuring the integrity of siRNA-carrier complexes in full human serum. J Control Release 126 67-76... 

Ivanov, V., J.B. Nagy, P. Lambin, A. Lucas, X.B. Zhang, The study of carbon nanotubules produced by catalytic method. Chem. Phys. Lett. 223, 329,1994. 

As mentioned earlier (see p. 122) the previously postulated dioxetane intermediate in firefly bioluminescence has been challenged as no 180 is in-corporated in the carbon dioxide released during oxidation of firefly luciferin with 18C>2. In view of the crucial significance of the 180. experiments De Luca and Dempsey 202> rigorously examined the reliability of their tracer method. They conclude from their experiments that all available evidence is in favour of a linear, not a cyclic peroxide intermediate — in contrast to Cypridina bioluminescence where at least part of the reaction proceeds via a cyclic peroxide (dioxetane) as concluded from the incorporation of 180 into the carbon dioxide evolved 202,203). However, the dioxetane intermediate is not absolutely excluded as there is the possibility of a non-chemiluminescent hydrolytic cleavage of the four-membered ring 204>. 

Lucas M, Mertens V, Corbisier A-M, Vanhulle S (2008) Synthetic dyes decolourisation by white-rot fungi development of original microtitre plate method and screening. Enzyme Microb Technol 42 97-106... 

Lucas L, Gilbert N, Ploton D, Bonnet N. Visualisation of volume data in confocal microscopy comparison and improvements of volume-rendering methods. JMicrosc 1996 181 238-252. 

If the product is an antibody, then it is essential to distinguish the immunoglobulin product, e.g., mouse IgG, from any media immunoglobulin components, e.g., bovine IgG. Lucas et al.16 developed an immunoassay to measure nanogram quantities of bovine IgG in the presence of a large excess of a structurally homologous protein, mouse MAb. The bovine IgG was a contaminant that copurified with the product from a protein A column. For the bovine IgG assay, whole IgG and protein A-purified IgG reacted differently in the assay. It is important to evaluate these types of assays for cross-reactivity. For other media components, such as chemicals or antibiotics, ELISA is probably not the most appropriate method due to the low immunogenicity of chemicals. Techniques such as HPLC would be better to detect these chemical components. 

Lucas, C., C. Nelson, M.L. Peterson, S. Frie, D. Vetterlein, T. Gregory, and A.B. Chen (1988). Enzyme-linked immunosorbent assays (ELISAs) for the determination of contaminants resulting from the immunoaffinity purification of recombinant proteins. J Immunol Methods 113(1) 113-122. 

In 2001, De Luca and GiacomeUi " reported a new simple and high-yielding one-flask synthesis of Weinreb amides from carboxylic acids and A-protected amino acids that uses different 1,3,5-triazine derivatives (such as 236) as the coupling agents (Scheme 104). The method allows the preparation of Weinreb amides 237 and hydroxamates as O-benzyl and 0-silyl hydroxamates that can be easily transformed into hydroxamic acids. 

Although pure silica and pure sodium carbonate would yield only soluble water glass, K. C. Bailey and A. Lucas believe that if the materials used by the Phoenicians contained lime as an impurity, true glass could possibly have been produced by the method described by Pliny (44). Many writers, however, regard Pliny s account as highly improbable. 

An HRTEM study of Zn-Cr Fe oxides using surface-profiling methods in the EM has been reported by Briscoe et al (1984, 1985). Chemical kinetic studies of the oxidation of benzene to maleic anhydride over V-Mo-O catalysts (prepared using 3 V205 Mo03), have been described using GC-MS techniques (Lucas et al 1983). However, microstructural information is limited and there are opportunities for EM studies of these catalyst systems. 

Lucas CP, Rigby JC, Lucas SB. The occurrence of depression following mania a method of predicting vulnerable cases. Br J Psychiatry 1989 154 705-708. 

Seiber JN, Glotfelty DW, Lucas AD, et al. 1990. A multiresidue method by high performance liquid chromatography-based fractionation and gas chromatographic determination of trace levels of pesticides in air and water. Arch Environ Contam Toxicol 19 583-592. 

Malosse, C.P., Ramirez-Lucas, D., and Rochat, J.M. 1995. Solid phase mictroextraction, an alternative method for the study of airborne insect pheromones (Metamasius hemipterus, Coleop-tera, Curculionidae). J. High Resolut. Chromatogr. 18 669-700. 

Lucas, P.W. and Luke, D.A. (1983). Methods for analysing the breakdown of food in human mastication. Arch. Oral Biol. 28, 813-819. 

Lucas HF. 1987. An improved method for the simultaneous determination of RA, Ra, and Ra in water soils and sediments. In Graves B, ed. Radon, radium, and other radioactivity in groundwater. Chelsea, Ml Lewis Publishers, 219-227. 

Stehney AF, Norris WP, Lucas HF, et al. 1955. A method for measuring the rate of elimination of radon in breath. Am J Roentgenol, Rad Therapy and Nuclear Med 73 774-784. 

Cp-substituted reactants. It is not clear why such long chain lengths are required to alleviate steric interactions in the Humphrey-Lucas molecules, but similar steric effects may be acting in the polymerizations of complex 3, where two molecules of 3 and a catalyst molecule all need to interact to carry out the polymerization. Nevertheless, despite this one setback, ADMET remains an attractive method for synthesizing new polymers with metal-metal bonds along their backbones. 

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