Cypridina Bioluminescence

In the lsO-incorporation experiment of Cypridina bioluminescence, the effects of the O atom exchange and contaminating CO2 are clearly seen in the relationship between the amount of luciferin luminesced and the amount of lsO atoms incorporated into the product CO2 (Fig. 1.14 Shimomura and Johnson, 1973a). The experiments were done in glycylglycine buffer, pH 7.8, the same buffer as chosen by DeLuca and Dempsey (1970). The total volume of the reaction mixture was 4 ml, with 40 ml of gas phase (see the reaction vessel in Fig. A.5 in the Appendix). The data of the luminescence reaction with 1802 gas in the H2160 medium indicates that at least 1 pmol of... 

During the period between 1916 and the early 1950s, Harvey, his associates and students made great efforts to study various aspects of the Cypridina bioluminescence, which was described by Harvey in his book Bioluminescence (1952). It should be pointed out here... 

Goto, T., and Fukatsu, H. (1969). Cypridina bioluminescence VII. Chemiluminescence in micelle solutions — A model system for Cypridina bioluminescence. Tetrahedron Lett., pp. 4299-4302. 

Kishi, Y., etal. (1966a). Cypridina bioluminescence I structure of Cypridina luciferin. Tetrahedron Lett., pp. 3427-3436. 

Shimomura, O., and Johnson, F. H. (1970a). Mechanisms in the quantum yield of Cypridina bioluminescence. Photochem. Photobiol. 12 291-295. 

Shimomura, O., Johnson, F. H., and Masugi, T. (1969). Cypridina bioluminescence light-emitting oxyluciferin-luciferase complex. Science 164 1299-1300. 

Toya, Y. (1992). Chemistry of Vargula (formerly Cypridina) bioluminescence. Nippon Nogei Kagaku Kaishi 66 742-747. 

Cypridina bioluminescence, 66, 69 firefly bioluminescence, 16 Diphyes, 334 Diplocardia, 335 Diplocardia longa, 216, 235, 240 Diplocardia luciferin, 235-238, 340, 342 See Earthworm luciferin Diplocladon, 337 Diplopoda (Millipedes), 307 Diplotrema, 235 Diptera, 1, 2, 25... 

In Latia bioluminescence the ketone 121 was detected as product. Similarly, the chemiluminescence of imidazol-pyridinones 125 represents a model for Cypridina bioluminescence 181>. 

As mentioned earlier (see p. 122) the previously postulated dioxetane intermediate in firefly bioluminescence has been challenged as no 180 is in-corporated in the carbon dioxide released during oxidation of firefly luciferin with 18C>2. In view of the crucial significance of the 180. experiments De Luca and Dempsey 202> rigorously examined the reliability of their tracer method. They conclude from their experiments that all available evidence is in favour of a linear, not a cyclic peroxide intermediate — in contrast to Cypridina bioluminescence where at least part of the reaction proceeds via a cyclic peroxide (dioxetane) as concluded from the incorporation of 180 into the carbon dioxide evolved 202,203). However, the dioxetane intermediate is not absolutely excluded as there is the possibility of a non-chemiluminescent hydrolytic cleavage of the four-membered ring 204>. 

Kishi Y, Goto T, Hirata Y, Shimomura O, Johnson FH. Cypridina bioluminescence I. structure of Cypridina luciferin. Tetrahedron Lett 1966 7 3427-36. 

Kishi Y, Goto T, Inoue S, Sugiura S, Kishimoto H. Cypridina bioluminescence... 

Goto T, Inoue S, Sugjura S, Nishikawa K, Isobe M, Abe Y. Cypridina bioluminescence V. Structure of emitting species in the luminescence of Cypridina luciferin and its related compounds. Tetrahedron Lett 1968 37 4035-8. 

The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence. 

Goto, T, Inoue, S. and Sugiura, S. (1968) Cypridina bioluminescence IV. Synthesis and chemiluminescence of 3,7-dihydroimidazo[l,2-a]pyrazin-3-one and its 2-methyl derivative. 

Kishi, Y., T. Goto, Y. Hkata, O. Shimomura, and F. H. Johnson Cypridina Bioluminescence I. Structure of Cypridina Luciferin. Tetrahedron Letts 1966, 3427. 

Shimomura, O., and F. H. Johnson Exchange of Oxygen Between Solvent H2O and CO2 Produced in Cypridina Bioluminescence. Biochem. Biophys. Res. Commun. 51, 558 (1973). 

Mshi, Y, Goto, T, Eguchi, S., Hirata, Y, Watanabe, F and Aoyama, T. (1966b) Cypridina bioluminescence II. Structural studies of Cypridina luciferin by means of high resolution mass spectrometer and an aminoacid analyser. Tetrahedron Lett., 7, 3437-3444. 

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