Liquid chromatography—mass pharmaceuticals

Brown, M.A., Liquid Chromatography/Mass Spectrometry Applications in Agricultural, Pharmaceutical and Environmental Chemistry, Oxford University Press, Oxford, 1998. 

Kerns, E. H., Di, L., Pemsky, S., Kleintop, T., Huryn, D., McConnell, O., Carter, G. Pharmaceutical profiling method for lipophilidty and integrity using liquid chromatography-mass spectrometry. 

The use of direct UV spectrophotometry to measure sample concentrations in pharmaceutical research is uncommon, presumably due to the prevalence and attractiveness of high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) methods. Consequently, most researchers are unfamiliar with the value of UV detection, mainly that it is generally much faster than other methods - a very important asset in high-throughput screening. 

High-Throughput Quantitative Pharmaceutical Analysis in Drug Metabolism and Pharmacokinetics Using Liquid Chromatography-Mass Spectrometry... 

R.D. Voyksner, C.S. Smith and P.C. Knox, Optimization and application of particle beam high-performance liquid chromatography/mass spectrometry to compounds of pharmaceutical interest, Biomed. Environ. Mass Spectrom., 19 (1990) 523-534. 

Pulsed ultrafiltration MS (PUF-MS) represents an inline high throughput affinity screening method with a variety of potential uses in the discovery and development of pharmaceuticals [22]. The in-line combination of solution-phase equilibration, ultrafiltration, and electrospray liquid chromatography mass spectrometry (LC-ESI-MS) facilitates the identification of high affinity target-specific... 

Bruins, A. P. (1991). Liquid chromatography-mass spectrometry with ionspray and electrospray interfaces in pharmaceutical and biomedical research. J. Chromatogr. 554 39-46. 

Wu Y. The use of liquid chromatography-mass spectrometry for the identification of drug degradation products in pharmaceutical formulations. Biomed Chromatogr 2000 14 384-396. 

Liquid chromatography/mass spectrometry (LC/MS)-based techniques provide unique capabilities for pharmaceutical analysis. LC/MS methods are applicable to a wide range of compounds of pharmaceutical interest, and they feature powerful analytical figures of merit (sensitivity, selectivity, speed of analysis, and cost-effectiveness). These analytical features have continually improved, resulting in easier-to-use and more reliable instruments. These developments coincided with the pharmaceutical industry s focus on describing the collective properties of novel compounds in a rapid, precise, and quantitative way. As a result, the predominant pharmaceutical sample type shifted from nontrace/pure samples to trace mixtures (i.e., protein digests, natural products, automated synthesis, bile, plasma, urine). The results of these developments have been sig-... 

Petrovic, M., M. Gros, and D. Barcelo. 2006. Multi-residue analysis of pharmaceuticals in wastewater by ultra-performance liquid chromatography-mass spectrometry. J. Chromatogr. A 1124 68-81. 

John H, Walden M, Schafer S, Genz S, Forssmann WG (2004) Analytical procedures for peptide quantification in pharmaceutical research by liquid chromatography-mass spectrometry. Anal Bioanal Chem 378 883-897... 

John, H., Walden, M., Schafer, S., Genz, S., Forssmarm, W.G. (2004). Analytical procedures for quantification of peptides in pharmaceutical research hy liquid chromatography mass spectrometry. Anal. Bioanal. Chem. 378 883-97. 

E Erni, Liquid chromatography-mass spectrometry in the pharmaceutical industry Objectives and needs, J. Chromatogr. 251 (1982), 141-151. 

A variety of MS formats are widely accepted and applied in the pharmaceutical industry. The specific MS application is often defined by the sample introduction technique. The pharmaceutical applications highlighted in this article feature two types of sample introduction techniques dynamic and static. Dynamic sample introduction involves the use of high-performance liquid chromatography (HPLC) on-line with MS. The resulting liquid chromatography/mass spectrometry (LC/MS) format provides unique and enabling capabilities for pharmaceutical analysis. The electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) modes are the most widely used. Static sample introduction techniques primarily use matrix-assisted laser desorption/ionization (MALDI). ... 

A. L. Yergey, C.G. Edmonds, LA S. Lewis, M.L. Vestal, Liquid Chromatography-Mass Spectrometry, Techniques and Applications, 1989, Plenum Press, New York, NY. M.A. Brown (Ed.), Liquid Chromatography-Mass Spectrometry, Applications in Agricultural, Pharmaceutical, and Environmental Chemistry, 1990, ACS Symposium Series, Vol 420, Washington, DC. 

Qualitative Analysis of Pharmaceuticals by Thermospray Liquid Chromatography/Mass... 

Liquid chromatography/mass spectrometry applications in agncultural Pharmaceutical, and environmental chemistry Mark A. Brown, editor. 

AP Bruins. Liquid chromatography—mass spectrometry with ion spray and electro spray interfaces in pharmaceutical and biomedical research. J Chromatogr 554(l-2) 39 -6, 1991. 

RS Plumb, GJ Dear, D Mallett, IJ Fraser, J Ayrton, C loannou. The application of fast gradient capillary liquid chromatography/mass spectrometry to the analysis of Pharmaceuticals in biofluids. Rapid Commun Mass Spectrom 13(10) 865-872,1999. 

Nogle, L.M. Mallis, L.M. Utilization of Multiplexed Liquid Chromatography/ Mass Spectrometry in the Purity and Accurate Mass Determination of Pharmaceutical Compound Libraries, in Proceedings ofthe 51st ASMS Conference on Mass Spectrometry and Allied Topics, Quebec, Canada, June 8-12, 2003. 

Plumb, R.S. Dear, G.J. Mallett, D. Fraser, LJ. Ayrton, J. loannou, C. TheApplication of Fast Gradient Capillary Liquid Chromatography/Mass Spectrometry to the Analysis of Pharmaceuticals in Biofluids, Rapid Commun. Mass Spectrom. 13, 865-872 (1999). 

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