Liquid chromatography-mass chemistry

Brown, M.A., Liquid Chromatography/Mass Spectrometry Applications in Agricultural, Pharmaceutical and Environmental Chemistry, Oxford University Press, Oxford, 1998. 

Methods based on liquid chromatography-mass spectrometry (LC-MS) and universally accepted search algorithms permit reliable identifications of low levels of proteins at high sensitivity [6]. Even semispecialized protein chemistry labs can readily identify proteins at the level of a few picomoles (10 pmol of a 50-kDa protein is 500 ng). Specialized groups with access to the latest advances in HPLC and mass spectrometry routinely work with subpicomolar quantities. Chemical proteomics as discussed here requires the more advanced equipment. 

The year 2002 was an extraordinary year for liquid chromatography-mass spectrometry (LC/MS) practitioners. On October 9, 2002, the Royal Swedish Academy of Sciences annonnced their decision to award the Nobel Prize in Chemistry to John B. Fenn, Koichi Tanaka, and Kurt Wiithrich for their development of analytical methods for the identification and structnral analysis of biological macromolecnles. Fenn and Tanaka shared the prize for developing electrospray and soft-laser desorption, respectively. These soft-ionization techniqnes allow macromolecules to be ionized withont fragmentation. 

Dohta, Y, Yamashita, T, Horiike, S., Nakamura, T. and Kukami, T. (2007) A system for logD screening of 96-weIl plates using a water-plug aspiration/ injection method combined with high-performance liquid chromatography-mass spectrometry. Analytical Chemistry, 79, 8312-8315. 

High-throughput liquid chromatography/Mass spectrometry method for the determination of the chromatographic hydrophobicity index. Analytical Chemistry, 73, 3716-3722. 

Lindsey M.E., M. Meyer, and E.M. Thurman (2001). Analysis of trace levels of sulfonamide and tetracycline antimicrobials in groundwater and surface water using solid-phase extraction and liquid chromatography/mass spectrometry. Analytical Chemistry 73 4640-4646. 

Wada, T., Nagasawa, E. and Hanaoka, S. (2006) Simultaneous determination of degradation products related to chemical warfare agents by high-performance liquid chromatography/mass spectrometry. Applied Organometallic Chemistry, 20(9), 573-79. 

Volume 59 Applications of Liquid Chromatography / Mass Spectrometry in Environmental Chemistry edited by D. Barcelo... 

Mellon, F. A. (1981). Gas chromatography-mass spectrometry and high performance liquid chromatography-mass spectrometry. In Mass Spectrometry (R. A. W. Johnstone, ed.), Vol. 6, pp. 196-232, A specialist periodical report of the Royal Society of Chemistry, London. 

R.M. Black and R.W. Read, Liquid chromatography/mass spectrometry in analysis of chemicals related to the chemical weapons convention, Encyclopedia of Analytical Chemistry, John Wiley Sons, Ltd, Chichester, 2000, 1007-1025. 

Pascoe R, Foley JP, Gusev Al (2001) Reduction in Matrix-Related Signal Suppression Effects in Electrospray Ionization Mass Spectrometry Using On-Line Two-Dimensional Liquid Chromatography. Analytical Chemistry 73 6014-6023... 

Robb DB, Covey TR, Bruins AP (2000) Atmospheric Pressure Photoionization An Ionization Method for Liquid Chromatography-Mass Spectrometry. Analytical Chemistry 72 3653-3659... 

A. L. Yergey, C.G. Edmonds, LA S. Lewis, M.L. Vestal, Liquid Chromatography-Mass Spectrometry, Techniques and Applications, 1989, Plenum Press, New York, NY. M.A. Brown (Ed.), Liquid Chromatography-Mass Spectrometry, Applications in Agricultural, Pharmaceutical, and Environmental Chemistry, 1990, ACS Symposium Series, Vol 420, Washington, DC. 

Liquid chromatography/mass spectrometry applications in agncultural Pharmaceutical, and environmental chemistry Mark A. Brown, editor. 

As FIA-MS and liquid chromatography mass spectrometry (LC-MS) become more pervasive in the analysis of compound libraries, open-access instrumentation is increasingly used in HTOS laboratories as well as in support of general medicinal chemistry. These open-access systems are most often used for reaction monitoring and optimization, and in some cases, for library quality control and synthesis product purification. 

This remains a non-trivial issue and represents one of the practical aspects in which sol-id-phase chemistry frequently poses a greater challenge than the corresponding solution-phase chemistry. High-throughput NMR spectroscopy and liquid chromatography-mass spectrometry (LC-MS) are now common place among the armamentarium of the synthetic chemist. 

Figure 6.31. Positive-ion MALDI-TOF reflectron mode mass spectrum of grape seed extract (matrix 2,5-dihydroxybenzoic acid). (Reprinted from Journal of Agricultural and Food Chemistry, 48, Yang and Chien, Characterization of grape procyanidins using high-performance liquid chromatography/mass spectrometry and matrix-assisted laser desorption time-of-flight mass spectrometry, p. 3993, Copyright 2000, with permission from American Chemical Society.)...

See also Capillary Electrochromatography. Capillary Electrophoresis Pharmaceutical Applications Low-Molecular-Weight Ions Food Chemistry Applications Clinical Applications. Extraction Solid-Phase Extraction Solid-Phase Microextraction. Gas Chromatography Mass Spectrometry. Liquid Chromatography Overview Liquid Chromatography-Mass Spectrometry. 

Figure 4 Atmospheric pressure chemical ionization source with corona discharge needle and heated nebulizer interface for combined liquid chromatography-mass spectrometry. (Reprinted with permission from Trends in Analytical Chemistry 13 (1994), 81 Elsevier.)...

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