Two-dimensional liquid chromatography 2D-LC

Two-dimensional-liquid chromatography (2D-LC) approaches are much easier to automate than 2D-electrophoresis. However 2D electrophoresis has the advantage that separation is performed at the protein and not at the peptide level and... 

Coupling of different chromatographic separation methods enables one to resolve multiple distinctive components. Two-dimensional liquid chromatography (2D-LC) has been shown to be an extremely useful tool for the investigation of the molecular heterogeneity of complex polymers [173-176], Until recently the application of 2D-LC was limited to ambient temperature and it was only in 2010 that the introduction of high-temperature 2D-LC was announced. 

The ultimate application of the LC-Transform system is to use it as a detector in two-dimensional liquid chromatography (2D-LC). Adrian et presented the deformulation of a... 

Ginzburg A, Macko T, Dolle V, Brull R. Characterization of polyolefins by comprehensive high-temperature two-dimensional liquid chromatography (HT 2D-LC). Euro Poly J 2011 47 319-29. 

The multidimensional distributions in hb polymers - that is, molar mass and DB-require the use of multidimensional separation techniques. For example, two-dimensional liquid chromatc raphy (2D-LC) [156] can provide simultaneous information on both molar mass and structural characteristics such as topol<, by combining SEC and interaction chromatography into one measurement. These investigations were conducted intensively for the separation of stars or long-chain branched polymers from linear analogs [157-160], and demonstrated the great potential of 2D-LC separations. Until now, the apphcation of 2D-LC has been... 

Holland and Jorgenson reported separating amines using anion exchange and reversed-phase columns in 1995 and since then, there have been numerous reports of combining two LC columns (2D-LC) to achieve efficient sample separation. In addition to the few references mentioned in this section, see Chapter 4 in this handbook on two-dimensional comprehensive liquid chromatography. 

In liquid chromatography, two dimensional separations in the vast majority of cases are not comprehensive. While comprehensive 2D-LC separations (LC X LC) can be accomplished and have been demonstrated (e.g., Refs. 30 and 31), the technique is not very popular. Probably one of the main reasons for this is the inability to perform very fast separations in liquid chromatography. In GC X GC, a typical second dimension separation can be completed in a few seconds. In LC, the separation time required is much longer. The problem can be overcome by stopping the flow in the first dimension column while the second dimension separation proceeds, but this causes the overall analysis times to be very long. 

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