Liquid chromatography/mass spectrometry throughput

Camurri, G., Zaramella, A. High-throughput liquid chromatography/mass spectrometry method for the determination of the chromatographic hydrophobicity index. Anal. Chem. 2001,... 

The use of direct UV spectrophotometry to measure sample concentrations in pharmaceutical research is uncommon, presumably due to the prevalence and attractiveness of high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) methods. Consequently, most researchers are unfamiliar with the value of UV detection, mainly that it is generally much faster than other methods - a very important asset in high-throughput screening. 

Xu, R. et al. 2002. Application of parallel liquid chromatography/mass spectrometry for high throughput microsomal stability screening of compound libraries. J. Am. Soc. Mass Spectrom. 13 155. 

High-Throughput Quantitative Pharmaceutical Analysis in Drug Metabolism and Pharmacokinetics Using Liquid Chromatography-Mass Spectrometry... 

Muckenschnabel, R. Falchetto, L. M. Mayr, I. Filipuzzi SpeedScreen label-free liquid chromatography-mass spectrometry-based high-throughput screening for the discovery of orphan protein ligands. Anal. Biochem. 2004, 324, 241-249. 

Pulsed ultrafiltration MS (PUF-MS) represents an inline high throughput affinity screening method with a variety of potential uses in the discovery and development of pharmaceuticals [22]. The in-line combination of solution-phase equilibration, ultrafiltration, and electrospray liquid chromatography mass spectrometry (LC-ESI-MS) facilitates the identification of high affinity target-specific... 

Niessen, W.M.A. Progress in liquid chromatography-mass spectrometry instrumentation and its impact on high-throughput screening. 

Kyranos, J. N. Cai, H. Wei, D. Goetzinger, W. K. High-throughput high-performance liquid chromatography/mass spectrometry for modem dmg discovery. Curr Opin Biotechnol 2001, 12, 105-111. 

J. W. Hirschelman, W. H. et ah Increasing the throughput and productivity of Caco-2 cell permeability assays using liquid chromatography-mass spectrometry application to resveratrol absorption and metabolism. Comb Chem High Throughput Screen 2003, 6, 757-767. 

Black, R. Henion, J. High-throughput selected reaction monitoring liquid chromatography-mass spectrometry determination of methylphenidate and its major metabolite, ritalinic acid, in rat plasma employing monolithic columns. J Chromatogr B Anal Technol Biomed Life Sci 2003, 783, 73-83. 

Important progress in terms of higher throughput in ADME/PK work was realized recently by wider use of liquid chromatography/mass spectrometry (LC/MS), which has now become a standard analytical tool [26]. Flow NMR spectroscopy has become a routine method to resolve and identify mixtures of compounds and has found applications in drug metabolism and toxicology studies [27]. 

Wilson, D. M., Wang, X., Walsh, E., and Rourick, R. A. (2001). High throughput log D determination using liquid chromatography-mass spectrometry. Comb. Chem. High Throughput Screen. 4 511-519. 

Simpson, H. Berthemy, A. Buhrman, D. Burton, R. Newton, J. Kealy, M. Wells, D. Wu, D. 1998. High throughput liquid chromatography/mass spectrometry bioanalysis using 96-well disk solid phase extraction plate for the sample preparation. Rapid Commun. Mass Spectrom., 12,75-82. 

H. Zhang, E. C. Yi, X.-J. Li, P. Mallick, K. S. Kelly-Spratt, C. D. Masselon, D. G. Camp, R. D. Smith, C. J. Kemp, and R. Aebersold, High throughput quantitative analysis of serum proteins using glycopeptide capture and liquid chromatography mass spectrometry, Mol. Cell. Proteomics, 4 (2005) 144—155. 

J. Isbell, R. Xu, Z. Cai, and D. B. Kassel, Realities of high-throughput liquid chromatography/mass spectrometry purification of large combinatorial libraries A report on overall sample throughput using parallel purification, 4 (2002), 600-611. 

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