High-performance liquid chromatography overview

The use of high performance liquid chromatography (HPLC) for the study of paralytic shellfish poisoning (PSP) has facilitated a greater understanding of the biochemistry and chemistry of the toxins involved. HPLC enables the determination of the type and quantity of the PSP toxins present in biological samples. An overview of the HPLC method is presented that outlines the conditions for both separation and detection of the PSP toxins. Examples of the use of the HPLC method in toxin research are reviewed, including its use in the determination of the enzymatic conversion of the toxins and studies on the movement of the toxins up the marine food chain. 

Bogusz, M., Franke, J. P., de Zeeuw, R. A., and Erkens, M., An overview of the standardization of chromatographic methods for screening analysis in toxicology by means of retention indices and secondary standards. Part II. High performance liquid chromatography, Fresenius ]. Anal. Chem., 347, 73, 1993. 

Khaledi, M. G., Micelles as separation media in high-performance liquid chromatography and high-performance capillary electrophoresis overview and perspective, /. Chromatogr. A, 780, 3, 1997. 

Liquid cathode lithium cells, 3 464-466 Liquid chromatography, 4 618—620 6 375, 440-468 14 233. See also High performance liquid chromatography Ion chromatography applications, 4 625-626 6 457-465 brief overview, 6 384-388 column switching, 6 446-447 columns, 4 623 derivatization, 6 447—448 detectors, 4 622-623 6 386-387, 448 52... 

This review provides an overview of the literature published to date on macrocyclic antibiotics exploited for enantioselective separations in high-performance liquid chromatography (HPLC). It was not intended as a comprehensive issue on the applications of such antibiotics in sub- and supercritical fluid chromatography (SFC), thin layer chromatography (TLC), capillary electrophoresis (CE), and capillary electrochromatography (CEC). A number of structural properties of the most important macrocyclic antibiotics applied in HPLC enantioseparations are listed in Table 2.1. 

An overview and discussion is given of literature methods published after 1989 devoted to the ion-interaction chromatographic determination of inorganic anions. Seventy references are quoted. Ion-interaction chromatography makes use of commercial reversed-phase stationary phase and conventional high-performance liquid chromatography instrumentation. The basis of the technique, the modification of the stationary phase surface, the choice of the ion-interaction reagent as well as the dependence of retention on the different variables involved are discussed. Examples of application in the fields of environmental, clinical and food chemistry are presented. The experimental conditions of stationary phase, of mobile phase composition as well as detection mode, detection limit and application are also summarized in tables. 1997 Elsevier Science B.V. 

L. S. Ettre, Evolution of liquid chromatography a historical overview. In High-Performance Liquid Chromatography (C. Horvath, ed.), Academic Press, New York, 1980, p. 54. 

The recent developments in the determination of acetylcholine and choline, the advantages and the disadvantages of a variety of analytical methods used in the analysis, were reviewed and discussed by Maruyama et al. [7]. Hanin published an overview for the methods used for the analysis and measurement of acetylcholine [8], Shimada et al. presented a review, including the applications of some reactors in the analysis of choline and acetylcholine. The immobilized enzyme reactors were used for detection systems in high performance liquid chromatography [9],... 

Based on the quantity of data and the complexity of the process, an enterprise operational informatics system must be robust and scalable. It should also integrate with analytical instrumentation such as high performance liquid chromatography (HPLC) MS to verify compound identity. A recent review of HTS informatics provides a compact overview of the key informatics elements of HTS operations and data handling (Ling, 2008). 

The characteristics of high performance liquid chromatography (HPLC) make it the method of choice for the separation of these compounds. Many improvements have been made in this field however, it was in the area of devices for detection and identification coupled to HPLC that more advances were made in recent years. These methods avoid the tedious and time-consuming isolation of compounds for the subsequent individual identification of each compound. In this paper, an overview of the analysis of phenolics by HPLC is provided. 

High-performance liquid chromatography (HPLC) is a versatile analytical technique using sophisticated equipment refined over several decades. An in-depth understanding of the working principles and trends is useful for more effective application of the technique. This chapter provides the reader with a concise overview of HPLC instrumentation, operating principles, recent advances, and modern trends. The focus is on the analytical scale HPLC systems and modules (pump, injector, and detectors). System dwell volume... 

LS Ettre. Evolution of Liquid Chromatography A Historical Overview. In Cs. Horvath, ed. High Performance Liquid Chromatography Advances and Perspectives. Vol. 1. New York Academic Press, 1980. 

Overview of high performance liquid chromatography instrumentation... 

As it already has been described in the overview, SDC is based on two well-polished surfaces, slid one at the top of the other generating a fluid flow thanks to the viscous drag. For chromatographic purposes, like in reverse-phase high-performance liquid chromatography (RP-HPLC), the sample solution (present in the mobile phase) needs to flow over a separation surface (the stationary phase) that can adsorb or absorb the sample... 

Creatinine and purine derivatives are frequently analyzed in human and veterinary clinical chemistry laboratories. We present here an overview of high-performance liquid chromatography (HPLC) and other current analytical methods, including photometric and enzymatic determinations, capillary electrophoresis (CE), and gas chromatography-mass spectrometry (GC-MS) for the measurement of these compounds. 

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