Lipid spectrometry

Murphy, R.C., Mass Spectrometry of Lipids, Plenum Press, New York, 1993. 

Murphy, R. C. Mass Spectrometry of Lipids. New York Plenum Press, 1993. Shibamoto, T., Ed. Lipid Chromatographic Analysis. New York Marcel Dekker, 1993. 

All of the above lipids can be separated from one another by techniques such as column, thin layer, and gas-liquid chromatography and their strucmres established by mass spectrometry. 

A glyceryl 2-aminoethylphosphonolipid has been isolated from Tetrahymenapyriformis and (45) has been detected by g.l.c.-mass spectrometry in both the lipid and proteinaceous fractions of human brain. The zwitterionic (45) was converted into volatile (46) by acetylation and methylation. Phosphonolipids derived from A-methyl-(45) have been synthesised by acetylation of A-methyl-(45) and subsequent conversion to the phosphorochloridate for the phosphorylation step. °... 

Dueker, S.R. et al.. Long-term kinetic smdy of P-carotene, using accelerator mass spectrometry in an adult volunteer, J. Lipid Res., 41, 1790, 2000. 

Microbes of differing physiologic types, acting in consortia, appear to be more destructive than monocultures. Methods for examining consortia are based on the detection of lipid biomarkers that are characteristic for different classes of microbes. These can be analyzed by gas chromatography coupled with mass spectrometry [512]. 

OES Acronym for optical emission spectrometry. oil A liquid lipid. 

Bethell, P. H., R. P. Evershed, and L. J. Goad (1993), The investigation of lipids, in Lambert, J. B. and G. Grufoe (eds.), Organic Residues by Gas Chromatography /Mass Spectrometry Applications to Palaeodietary Studies, Archaeology at the Molecular Level, Springer, Berlin, pp. 227-255. 

Drucker, D. B. Aluyi, H. S. Boote, V. Wilson, J. M. Ling, Y. Polar lipids of strains of Prevotella, Bacteroides and Capnocytophaga analyzed by fast atom bombardment mass spectrometry. Microbios 1993, 75, 45-56. 

Schneiter, R. Brugger, B. Sandhoff, R. Zellnig, G. Leber, A. Lampl, M. Athenstaedt, K. Hrastnik, C. Eder, S. Daum, G. Paltauf, F. Wieland, F. T. Kohlwein, S. D. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J. Cell Biol. 1999,146,741-754. 

The results for bacterial whole-cell analysis described here establish the utility of MALDI-FTMS for mass spectral analysis of whole-cell bacteria and (potentially) more complex single-celled organisms. The use of MALDI-measured accurate mass values combined with mass defect plots is rapid, accurate, and simpler in sample preparation then conventional liquid chromatographic methods for bacterial lipid analysis. Intact cell MALDI-FTMS bacterial lipid characterization complements the use of proteomics profiling by mass spectrometry because it relies on accurate mass measurements of chemical species that are not subject to posttranslational modification or proteolytic degradation. 

Gharaibeh, A. A. Voorhess, K. J. Characterization of lipid fatty acids in whole-cell microorganisms using in situ supercritical fluid derivatization/extraction and gas chromatography/mass spectrometry. Anal. Chem. 1996,68,2805-2810. 

Al-Saad, K. A. Zabrouskov, V. Siems, W. F. Knowles, N. R. Hannan, R. M. Hill, H. H. Matrix assisted laser desorption/ionization time-of-flight mass spectrometry of lipids ionization and prompt fragmentation patterns. Rapid Comm. Mass Spec-trom. 2003,17, 87-96. 

Basile, F. Beverly, M. B. Voorhees, K. J. Hadfield,T. L. Pathogenic bacteria Their detection and differentiation by rapid lipid profiling with pyrolysis mass spectrometry. Trac-Trends Anal. Chem. 1998,17,95-109. 

Volume 432. Lipidomics and Bioactive Lipids Mass-Spectrometry—Based Lipid Analysis (in preparation)... 

F.O. Giilagar, A. Buchs, A. Susini, Capillary gas chromatography mass spectrometry and identification of substituted carboxylic acids in lipids extracted from a 4000 year old Nubian burial, Journal of Chromatography, 479, 61 72 (1989). 

Direct Mass Spectrometry to Characterise Wax and Lipid Materials... 

In the present chapter, we first provide some general information concerning the chemistry of waxes and lipids currently encountered in various items from our cultural heritage and we detail the main protocols based on direct mass spectrometry that have been developed so far. We then discuss the mass spectra obtained by EI-MS on a range of reference substances and materials sampled from museum and archaeological artefacts. We then focus on the recent possibilities supplied by electrospray ionisation for the elucidation of the structure of biomarkers of beeswax and animal fats. 

Investigations based on direct mass spectrometry analysis aiming at identifying waxes and other lipids in museum or archaeological items were first carried out at the end of... 

Figure 4.1 Analytical strategy in which direct mass spectrometry analyses using either elec tron ionisation or electrospray are used for detecting and identifying lipid substances in archaeological and museum samples...

---