Lipid oxidation measurement

The oil stability index (OSI) method provides results in a matter of hours instead of months (required for studies done at ambient temperatures). These OSI results are useful as comparative measures of oxidative stability, i.e., to determine the effect of a treatment or antioxidant compared to a control sample. Meaningful predictions of the actual shelf lives of specific commodities require that such shelf life studies be performed at ambient conditions. If only accelerated tests are to be performed, two or more tests based on different principles of lipid oxidation measurement should be conducted the effect of accelerated storage temperature should also be investigated. 

Jenkins, D.J.A., Kendall, C.W.C., and Vidgen, E. 1999. Health aspects of partially defatted flaxseed, including effects on serum lipids, oxidative measures, and ex vivo androgen and progestin activity A controlled crossover trial. Am. J. Clin. Nutr. 69, 395-402. 

Flavor Components of Fats and Oils, Lipid Oxidation Measurement Methods, Marine Mammal Oils, Modification of Fats and Oils via Chemical and Enzymatic Methods, Novel Separation Techniques for Isolation and Purification of Fatty Acids and Oil By-Products, Quality Assurance of Fats and Oils, Tree Nut Oils. 

Ying Zhong Memorial University of Newfoundland, St. John s, Newfoundland, Canada, Antioxidants Regulatory Status , Citrus Oils and Essences , Lipid Oxidation Measurement Methods , Marine Mammal Oils. 

Table III. Induction Time of Lipid Oxidation Measured by Oxidative...

Smiddy M., Fitzgerald M., Kerry J.P., Papkovsky D.B., O Sullivan C.K., Buckley D.J., Guilbault G.G., Use of oxygen sensors to non-destructively measure the oxygen content in modified atmosphere and vacuum packed beef impact of oxygen content on lipid oxidation, Meat Science 2002 61 285-290. 

Decomposition of the primary products of lipid oxidation generates a complex mixture including saturated and unsaturated aldehydes such as hexanal. Hexanal is the most commonly measured end product of lipid oxidation, and both sensory and physicochemical methods are used for its determination. Where other antioxidant activity tests may be nonspecific, physicochemical measurement of hexanal offers the advantage of analyzing a single, well-defined end product. 

A simple method for assessing lipid oxidation is measuring the headspace concentration of hexanal by capillary GLC. Also, the total volatiles appearing in the chromatogram up to hexanal can be taken as oxidation index. The method was applied to determine the amounts of lipid peroxides present in rat liver cells. Enhancement of the hexanal concentration can be achieved on adding ascorbic acid (22), that reduces Fe(ni) present in the matrix to Fe(II), which catalyzes decomposition of hydroperoxides to aldehydes. Significant correlations are found between hexanal concentrations and various oxidation indices, such as TBARS (Section IV.D.2)" . ... 

Interest has been shown in using derivatives of ascorbic acid as antioxidants. One such compound is ascorbyl palmitate. In the structure of ascorbyl palmitate, the 2- and 3-positions are occupied by hydroxyl groups the 6-position contains the substituted fatty acid. Other derivatives, synthesized by Seib and associates were ascorbate-2 phosphate and ascorbate 2-triphosphate (27). Both of these compounds were reported to inhibit lipid oxidation in ground meat as measured by chemical means (2). Ascorbate-2-phosphate was also found to inhibit MFD in beef as measured by sensory means (2). The ascorbate-2-triphosphate was not tested as an inhibitor of MFD in this study, but... 

Haugen, E., Undeland, I. (2003) Lipid oxidation in herring fillets (Clupea harengus) during ice storage measured by a commercial hybrid gas sensor array system. J. Agric. Food Chem. 51 752-759. 

D2.4 Spectrophotometric Measurement of Secondary Lipid Oxidation Products... 

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