Measurements for Oxidative Stress and Lipid Peroxidation

These measurements include the F2 isoprostanes—the oxidation products of arachi-donic acid—and methods require mass spectrometry or immunoassay. Other measures of lipid peroxidation include hydroperoxides by luminometry or colorimetric assays, or a third colorimetric measurement of aldehyde products (TEAR) with thiobarbituric acid where malondialdehyde is measured malondialdehyde is an end-product derived from the breakdown of polyunsaturated fatty acids and esters. 

Other measures for monitoring oxidative stress include superoxide dismutase, glutathione peroxidase, catalase, glutathione, glutathione disulphide, and glutathione reductase. The oxidation of LDL has been implicated in the development of cardiovascular diseases and diabetes. Oxidized LDL can be measured using 

Alexander, C., and C. E. Day. 1973. Distribution of serum lipoproteins of selected vertebrates. 

Aubert, R., D. Perdereau, M. Roubiscoul, J. Herzog, and D. Lemmonier. 1988. Genetic variations in serum lipid levels of inbred mice and response to hypercholesterolemic diet. Lipids 23 48-54. 

Scholey, L. Delacruz, M. Moret, and S. Nichol. 1991. Renal and urinary lipid changes associated with an acutely induced renal papillary necrosis in rats. Food Chemistry and Toxicology 29 211-219. 

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