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Ligases

Ligases (syniheiases). Enzymes catalysing the joining together of two molecules coupled with the hydrolysis of a pyrophosphate bond in ADP or a similar triphosphate. They include some carboxylases and many enzymes known as synthetases. [Pg.159]

The synthetic and plasmid DNAs are mixed and join their sticky ends spontaneously. They are covalently bound together by DNA ligases, when the resulting hybrid plasmid is inserted into bacterial cells. Dilute calcium chloride solutions render the bacterial membranes permeable and allow the passage of ONA into the cells. [Pg.243]

DNA fibers DNA fingerprinting DNA gyrase DNA ligase DNA ligases DNA polymerase... [Pg.340]

Synthesize second strand with T7 DNA polymerase and T4 ligase... [Pg.199]

Seal gaps in chimeric plasmid widi DNA ligase... [Pg.398]

FIGURE 13.4 Blunt-end ligation using phage T4 DNA ligase, which catalyzes the ATP-dependent ligation of DNA molecules. AMP and PP are by-products. [Pg.399]

Enzymes are classified into six categories depending on the kind of reaction they catalyze, as shown in Table 26.2. Oxidoreductases catalyze oxidations and reductions hansferases catalyze the transfer of a group from one substrate to another hydrolases catalyze hydrolysis reactions of esters, amides, and related substrates lyases catalyze the elimination or addition of a small molecule such as H2O from or to a substrate isomerases catalyze isomerizalions and ligases catalyze the bonding together of two molecules, often coupled with the hydrolysis... [Pg.1041]

Lidocaine, molecular model of, 101 Ligase, 1041-1042 Light, plane-polarized, 294 speed of, 420... [Pg.1303]

Scheme 10.32 Examples of reactions catalyzed by different classes of dehalogenases. HD haloalcohol dehalogenase EH epoxide hydrolase CL p-chlorobenzoyl-CoA ligase CBD p-chlorobenzoyl-CoA dehalogenase. Scheme 10.32 Examples of reactions catalyzed by different classes of dehalogenases. HD haloalcohol dehalogenase EH epoxide hydrolase CL p-chlorobenzoyl-CoA ligase CBD p-chlorobenzoyl-CoA dehalogenase.
Acyl-CoA synthetases are enzymes (i.e., ligases) that convert fatty acid molecules into acyl-Coenzyme A molecules for their subsequent oxidation. [Pg.14]

Adaptor Proteins. Figure 1 Adaptor protein domains. A scheme of the domain structures of some well-characterized adaptor proteins is shown. Descriptions of domain characteristics are in main text except C2, binds to phospholipids GTPase activating protein (GAP) domain, inactivates small GTPases such as Ras Hect domain, enzymatic domain of ubiquitin ligases and GUK domain, guanylate kinase domain. For clarity, not all domains contained within these proteins are shown. [Pg.15]

WW domains (named after the one letter abbreviation for the amino acid tryptophan) are small regions of around 30 residues, which, like SH3 domains, bind to polyproline sequences. These sequences often contain the consensus sequence PPXY or PPLP. Examples of proteins that contain WW domains include Nedd4 E3 ubiquitin ligase (Fig. 1) and IQGAP1. [Pg.18]


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4-Coumaroyl:CoA-ligase

4-coumarate: CoA ligase

5-lactone ligase

ATP-dependent synthetases and ligases

Acetate-CoA ligase

Acid-Thiol Ligases and CoA-Transferases

Acid-ammonia ligases

Acid-thiol ligases

Acid: CoA ligase

Acyl-CoA ligases

Alanine ligase

Amino acid tRNA ligases

Aminoacyl-tRNA ligases

Ammonia ligase

Apparent equilibrium constants of ligase reactions

Biotin ligase

Biotin protein ligase

C-N ligase

COP9 ubiquitin ligases

Cinnamate : CoA ligase

CoA ligases

Combined ligase chain reaction

Cullin ring ligase

Cyclization ligase catalyzed

Cyclo-ligases

DNA ligase

DNA ligase mechanism

DNA ligases

DNA, hypomethylation ligase

Deoxyribonucleic acid ligase

Deoxyribonucleic acid ligase role in vivo

Deoxyribonucleic acid ligases

E3 Ligase

E3 ubiquitin ligase

E4 ligase

Escherichia coli DNA ligase

Fatty acid-CoA ligase

Fatty acid: coenzyme A ligases

Fatty acyl-CoA ligase

Glutamate ammonia ligase

Glutamate-cysteine ligase

Glutamate-cysteine ligase modifier

Glutamine ammonia ligase

Glutamylcysteine ligase

Glutathione glutamate cysteine ligase

L-Aspartate ligase

LI ligase ribozyme

Ligase

Ligase

Ligase 4-coumarate

Ligase aminoacyl

Ligase bacterial

Ligase chain reaction

Ligase cytosolic

Ligase definition

Ligase detection reaction

Ligase enzyme

Ligase eukaryotic

Ligase mRNA

Ligase messenger

Ligase reactions

Ligase ribozymes

Ligases chain reaction

Ligases coenzymes

Ligases enzymes

Ligases nomenclature

Ligases, defined

Lipoic acid ligase

Long-chain fatty-acid-CoA ligase

Mammalian DNA ligases

P-coumarate:CoA ligase

Peptide ligase

Peptide ligases

Phosphoprotein-ubiquitin ligase

Phosphoprotein-ubiquitin ligase complexes

Polynucleotide ligase

RNA ligase

RNA ligases

Ribonucleases ligase

SUMO E3 ligase

Salicylate-AMP ligase

Succinate-CoA ligase

Succinate-CoA ligases

T4 DNA ligase

T4 RNA ligase

T4 ligase

The SCF Ubiquitin E3 Ligase

The Structural Biology of Ubiquitin-Protein Ligases

Tubulin-tyrosine ligase

Ub ligase

Ub-protein ligases

Ubiquitin ligase

Ubiquitin ligase XIAP

Ubiquitin ligases

Ubiquitin protein ligase

Ubiquitin protein ligases

Ubiquitin-conjugating ligases

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