Ligase mRNA

Kuhn, D.N., Chappell, J., Boudet, A. Hahlbrock, K. (1984). Induction of phenylalanine ammonia-lyase and 4-coumarate CoA ligase mRNAs in cultured plant cells by UV light or fungal elicitor. Proceedings of the National Academy of Sciences, USA, 81,1102-6. 

Both molecules are folded in such a way that the 3 end and the 5 end are close together. As in DNA, most of the bases are located in the inside of the structures, while the much more polar backbone is turned outwards. An exception to this is seen in the three bases of the anticodon of the tRNA (pink), which have to interact with mRNA and therefore lie on the surface of the molecule. The bases of the conserved CCA triplet at the 3 end (red) also jut outward. During amino acid activation (see p.248), they are recognized and bound by the ligases. 

Sidrauski, C., Cox, J.S., and Walter, P., 1996, tRNA Ligase is required for regulated mRNA splicing in the unfolded protein response. Cell 87 405—113. 

Restriction endonucleases and DNA ligase Cutting, rejoining DNA Reverse transcriptase Conversion of mRNA into cDNA Chemical DNA synthesis Oligonucleotides Polymerase chain reaction (PCR)... 

RNA ligase An enzyme that catalyses exon rejoining in the splicing of certain mRNAs. 

Fig. 5.5. Outline of a PCR-based procedure for measuring the length of the poIy(A) tail. Total RNA is hybridised with a saturating amount of phosphorylated olig-o(dT)i2-is at 42°C in the presence of T4 DNA ligase resulting in a complementary poly(dT) tract except at the 3 -terminus of the mRNA. The temperature is then...

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